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Overview of Fluorophores

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Immunocytochemical Methods and Protocols

Part of the book series: Methods in Molecular Bilogy ((MIMB,volume 34))

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Abstract

Since its inception in the 1940s, the technique of immunofluorescence has provided a sensitive, high-resolution method for analyzing the binding of antibody to antigen. Fluorescent molecules, termed fluorophores or fluorochromes, can be conjugated directly to antibodies by covalent linkage, or coupled indirectly to antibodies via conjugation to proteins A and G or through an avidin-biotin bridge (see Chapter 6,Chapter 7,Chapter 21, and Chapter 22). The basic features of immunofluorescence are straightforward, but a working knowledge of the commonly used fluorophores is of value in obtaining maximum performance from immunofluorescence microscopy and flow cytometry.

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Authors and Affiliations

  1. Department of Biology, The Catholic University of America, Washington, DC

    J. Michael Mullins

Authors
  1. J. Michael Mullins
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Editor information

Editors and Affiliations

  1. The Catholic University of America, Washington, DC

    Lorette C. Javois

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© 1994 Humana Press Inc.

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Michael Mullins, J. (1994). Overview of Fluorophores. In: Javois, L.C. (eds) Immunocytochemical Methods and Protocols. Methods in Molecular Bilogy, vol 34. Humana Press. https://doi.org/10.1385/0-89603285-X:107

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  • DOI: https://doi.org/10.1385/0-89603285-X:107

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-285-9

  • Online ISBN: 978-1-59259-521-1

  • eBook Packages: Springer Protocols

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