Overview of Flow Cytometry and Fluorescent Probes for Cytometry

  • Robert E. Cunningham
Protocol
Part of the Methods in Molecular Bilogy book series (MIMB, volume 34)

Abstract

It was shown by Creech and Jones (1) in 1940 that proteins, including antibodies, could be labeled with a fluorescent dye (phenylisocyanate) without biological or immunological effects to the intended target. In theory, fluorescent reporters (tracers, probes, antibodies, stains, and so on) can be used to detect or measure any cell constituent, provided that the tag reacts specifically and stoichiometrically with the cellular constituent in question (2). Today, the repertoire of fluorescent probes is expanding almost daily (see also  Chapter 14). One area that has benefited from the ever increasing number of fluorescent probes is flow cytometry.

Keywords

Bromide Iodide Ethidium Sorting DAPI 

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Copyright information

© Humana Press Inc. 1994

Authors and Affiliations

  • Robert E. Cunningham
    • 1
  1. 1.Department of Cellular PathologyArmed Forces Institute of PathologyWashington

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