Abstract
Immunoassays are techniques for measuring the concentration or activity of a substance using immunological reactions. Several types of immunoassay are commonly used, including precipitation assays using antibody/antigen complexes, agglutination assays using coated erythrocytes or other particles, and radio-(RIA) and enzyme immunoassays. Multiple factors determine the best assay for a particular use, including sample concentration, technical difficulty, required precision, and availability of specialized equipment. The most sensitive assays are the radio- and enzyme immunoassays, however safety and environmental issues regarding the use of radioisotopes have limited the application of RIAs, and thus enzyme-linked immunosorbent assays (ELISA) tend to be the method of choice when sample concentration is a critical issue. The potential for robotic automation and the availability of inexpensive microplate dilutors, pipetors, and readers has allowed for the generation of highly reproducible results for large numbers of samples in an short time.
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References
Rubenstein, K. E., Schneider, R. S., and Ullman, E. F. (1972) Homogeneous enzyme-immunoassay. A new immunochemical technique. Biochem. Biophys. Res. Commun. 47, 846–858.
Ullman, E. F. and Maggio, E. T. (1980) Principles of homogeneous enzyme-immunoassay, in Enzyme-immunoassay (Maggio, E. T., ed.), CRC, Boca Raton, FL, pp. 106–131.
Crowther, J. R. (1995) ELISA: Theory and Practice. Humana, Totowa, NJ.
Pollock, P. L., Germolec, D. R., Comment, C. E., Rosenthal, G. J., and Luster, M. I. (1994) Human lymphocyte engrafted SCID mice as a model for immunotoxicity assessment: studies with cyclosporine A and TCDD. Fundam. Appl. Toxicol. 22, 130–138.
Kemeny, D. M. (1990) A Practical Guide to ELISA. Pergamon, Oxford.
Carpenter, A. B. (1992). Enzyme-linked immunoassays, in Manual of Clinical Laboratory Immunology, 4th ed. (Rose, N. R., de Macario, E. C, Fahey, J. L., Friedman, H., and Penn, G. M., eds.), American Society for Microbiology, Washington, DC, pp. 2–9.
Macy, E., Kemeny, M., and Saxon, A. (1988) Enhanced ELISA: how to measure less than 10 picograms of a specific protein (immunoglobulin) in less than 8 hours. FASEB J. 2, 3003–3009.
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© 1999 Humana Press Inc., Totowa, NJ
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Germolec, D.R. (1999). The Use of Enzyme-Linked Immunosorbent Assays to Quantitate Proteins in Biological Functions. In: Harry, J., Tilson, H.A. (eds) Neurodegeneration Methods and Protocols. Methods in Molecular Medicine™, vol 22. Humana Press. https://doi.org/10.1385/0-89603-612-X:191
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DOI: https://doi.org/10.1385/0-89603-612-X:191
Publisher Name: Humana Press
Print ISBN: 978-0-89603-612-3
Online ISBN: 978-1-59259-604-1
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