Methodological Issues in Polymerase Chain Reaction for RNA
In order to understand the functionality of genes and the importance of their expression, it is important to be able to understand proteins and, therefore, amino acids. Many techniques allow one to look at proteins at the amino acid level; however, very few allow researchers to understand them at the nucleic acid level. It is obvious that in order to study proteins and amino acids at the nucleic acid level, one would need to look at RNA. Unfortunately, because of the nature of RNA (its single-stranded nature) it is a very labile substrate and difficult to study, especially if the RNA of interest is present in cells at a low abundance.
KeywordsPolymerase Chain Reaction Polymerase Chain Reaction Amplification Random Hexamers Single Tube Avian Myeloblastosis Virus
- 1.Maniatis, T. (1982) Molecular Cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.Google Scholar
- 4.Powell, J. C. (1989) Measurement of mRNA by quantitative PCR with a non-radioactive label. J. Lipid Res. 33, 609–614.Google Scholar
- 5.Myers, T. (1992) Enzymatic properties of a DNA polymerase from thermus thermophilus on RNA and DNA templates. J. Cell. Biochem. 16B, 29.Google Scholar
- 8.Nuovo, G. J. (1994) PCR In Situ Hybridization: Protocol and Applications. Raven, New York.Google Scholar