Astrocyte and Neuron Coculturing Method

  • Michael Aschner
  • Barbara A. Bennett
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 22)


It is customary to credit Rudolf Virchow (1) with the discovery of neuroglia (see ref. 2). As a practicing pathologist who was familiar with inflammatory processes in the brain, Virchow opposed his contemporaries’ assertion that the brain was void of connective tissue. He hypothesized that underneath the single-cell layer of the ependyma, the ventricles were lined with connective tissue cells that were capable of mounting inflammatory responses, and referred to these cells as “Nervenkitt” or “nerve putty.” Although erroneous, this coined term has persisted as the preferred generic term, or in its shortened form “glia,” for a class of nonexcitable brain cells. Classification and histological characterization of the true nature of the various neuroglial types followed the development of impregnation techniques by Golgi and Ramon y Cajal in the late 1800s. By the 1920s, the major forms of glial cells had been recognized and identified. Their basic structures and relationships with other critical parts of the nervous system were also beginning to emerge.


Glial Fibrillary Acidic Protein Primary Astrocyte Culture Conical Centrifuge Tube Cellular Yield Curve Forceps 
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  1. 1.
    Virchow, R. (1846) Ueber das granulierte Ansehsn der Wandungen der Gehirnventrikel. Allg. Z. Psychiatrie 3, 242–250.Google Scholar
  2. 2.
    Somjen, G. G. (1988) Nervenkitt: notes on the history of the concept of neuroglia. Glia 1, 2–9.PubMedCrossRefGoogle Scholar
  3. 3.
    Lugaro, E. (1907) Sulle Funzioni Delia Nevroglia. Riv. D. Pat. Nerv. Merit. 12, 222–233.Google Scholar
  4. 4.
    Kimelberg, H. K. and Aschner, M. (1994) Astrocytes and their functions, past and present, in National Institute on Alcohol Abuse and Alcoholism Research Monograph 27, Alcohol and Glial Cells. NIH Publication No. 94-3742, Bethesda, MD, pp. 1–40.Google Scholar
  5. 5.
    Kuffler, S. W., Nicholls, G., and Orkand, K. (1966) Physiological properties of glial cells in the CNS system of amphibia. J. Neurophysiol. 29, 768–787.PubMedGoogle Scholar
  6. 6.
    Booher, J. and Sensenbrenner, M. (1972) Growth and cultivation of dissociated neurons in glial cells from embryonic chick, rat and human brain in flask cultures. Neurobiology 2, 97–105.PubMedGoogle Scholar
  7. 7.
    Eng, L. F., Vanderhaeghen, J. J., Bignami, A., and Gerstl, B. (1971) An acidic protein isolated from fibrous astrocytes. Brain Res. 28, 351–354.PubMedCrossRefGoogle Scholar
  8. 8.
    Bignami, A. and Dahl, D. (1976) The astroglial response to stabbing: immunofluorescence studies with antibodies to astrocyte-specific protein (GFA) in mammalian and submammalian vertebrates. Neuropathol. Appl. Neurobiol. 2, 99–110.CrossRefGoogle Scholar
  9. 9.
    McCarthy, K. D. and De Vellis, J. (1980) Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue. J. Cell Biol. 85, 890–902.PubMedCrossRefGoogle Scholar
  10. 10.
    Frangakis, M. V. and Kimelberg, H. K. (1984) Dissociation of neonatal rat brain by dispase for preparation of primary astrocyte cultures. Neurochem. Res. 9, 1689–1698.PubMedCrossRefGoogle Scholar
  11. 11.
    Kimelberg, H. K. and Norenberg, M. D. (1989) Astrocytes. Sci. Amer. 260, 66–76.CrossRefGoogle Scholar
  12. 12.
    Abbott, N. J., ed. (1991) Glial-Neuronal Interactions. Annals of the New York Academy of Science, New York, pp. 663.Google Scholar
  13. 13.
    Murphy, S., ed. (1993) Astrocytes: Pharmacology and Function. Academic, New York.Google Scholar
  14. 14.
    Aschner, M. and Kimelberg, H. K., eds. (1996) The Role of Glia in Neurotoxicity. CRC, Boca Raton, FL.Google Scholar
  15. 15.
    Rosenberg, P. A. and Aizenman, E. (1989) Hundred-fold increase in neuronal vulnerability to glutamate toxicity in astrocyte-poor cultures of rat cerebral cortex. Neurosci. Lett. 103, 162–168.PubMedCrossRefGoogle Scholar
  16. 16.
    Vallee, R. B., Bloom, G. S., and Luca, F. C. (1986) Differential structure and distribution of the high molecular weight brain microtubule-associated proteins, MAP-1 and MAP-2. Ann. NY Acad. Sci. 466, 134–144.PubMedCrossRefGoogle Scholar
  17. 17.
    Kimelberg, H. K., Narumi, S., and Bourke, R. S. (1978) Enzymatic and morpho-logical properties of primary rat brain astrocyte cultures, and enzyme development in vivo. Brain Res. 153, 55–77.PubMedCrossRefGoogle Scholar
  18. 18.
    Manthorpe, M., Adler, R., and Varon, S. (1979) Development, reactivity and GFA immunofluorescence of astroglia-containing monolayer cultures from rat cere-brum. J. Neurocytol. 8, 605–621.PubMedCrossRefGoogle Scholar
  19. 19.
    Levison, S. W. and Goldman, J. E. (1993) Astrocyte origins, in Astrocytes: Pharmacology and Function (Murphy, S., ed.), Academic, New York, pp. 1–22.Google Scholar
  20. 20.
    Hertz, L., Juurlink, B. H. J., Szuchet, S., and Walz, W. (1985) Cell and tissue culture, in Neuromethods 1: General Neurochemical Techniques (Boulton, A. A. and Baker, G. B., eds.), Humana, Clifton, NJ, pp. 117–167.Google Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 1999

Authors and Affiliations

  • Michael Aschner
    • 1
  • Barbara A. Bennett
    • 1
  1. 1.Department of Physiology and PharmacologyBowman Gray School of MedicineWinston-Salem

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