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Construction of Mouse Adenovirus Type 1 Mutants

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Adenovirus Methods and Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 21))

Abstract

Construction of mouse adenovirus type 1 (MAV-1) mutants is facilitating studies of adenoviral pathogenesis in the natural host. We have isolated the first site-directed viral mutants of MAV-1, and we are comparing effects of wild-type (wt) and these mutant viruses in infections of mice (Smith et al., submitted; Ying et al., submitted; refs. 1 and 2). The mutants have lesions in viral genes early region 1 (E1) or 3 (E3), and have altered lethality in adult mice.

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Author information

Authors and Affiliations

  1. Department of Genetics, University of Georgia, Athens, GA

    Angela N. Cauthen & Katherine R. Spindler

Authors
  1. Angela N. Cauthen
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  2. Katherine R. Spindler
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Editor information

Editors and Affiliations

  1. St. Louis University Health Science Center, St. Louis, MO

    William S. M. Wold

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© 1999 Humana Press Inc., Totowa, NJ

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Cauthen, A.N., Spindler, K.R. (1999). Construction of Mouse Adenovirus Type 1 Mutants. In: Wold, W.S.M. (eds) Adenovirus Methods and Protocols. Methods in Molecular Medicine™, vol 21. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-551-4:85

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  • DOI: https://doi.org/10.1385/0-89603-551-4:85

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-551-5

  • Online ISBN: 978-1-59259-603-4

  • eBook Packages: Springer Protocols

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