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Large-Scale Purification and Crystallization of Adenovirus Hexon

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Adenovirus Methods and Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 21))

Abstract

Adenovirus, shortly after its discovery in 1953 (1), was one of the first biological entities to be imaged using the electron microscope (EM) (2). Since then, It has been under continuous scrutiny by structural biologists (reviewed inref. 3). The characteristic shape of the adenovirus virion, with its projecting fibers, made it a fascinating object for early EM studies, which focused on how its > 1O structural proteins are organized to form the virion. The size of the virion, with a diameter of 914å at the fivefold vertices (4) and a mass of over 150 × 1O6 Dalton, presents a formidable challenge for X-ray crystallographic studies.

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Author information

Authors and Affiliations

  1. Department of Biochemistry/Sciences II, University of Geneva, Switzerland

    John J. Rux & Donatelia Pascolini

  2. The Wistar Institute, Philadelphia, PA

    Roger M. Burnett

Authors
  1. John J. Rux
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  2. Donatelia Pascolini
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  3. Roger M. Burnett
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Editor information

Editors and Affiliations

  1. St. Louis University Health Science Center, St. Louis, MO

    William S. M. Wold

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© 1999 Humana Press Inc., Totowa, NJ

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Rux, J.J., Pascolini, D., Burnett, R.M. (1999). Large-Scale Purification and Crystallization of Adenovirus Hexon. In: Wold, W.S.M. (eds) Adenovirus Methods and Protocols. Methods in Molecular Medicine™, vol 21. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-551-4:259

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  • DOI: https://doi.org/10.1385/0-89603-551-4:259

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-551-5

  • Online ISBN: 978-1-59259-603-4

  • eBook Packages: Springer Protocols

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