Cell Survival and Apoptosis Assays for E1B 19K and Bcl-2 Family Members

  • T. Subramanian
  • G. Chinnadurai
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 21)


Programmed cell death, or apoptosis, is an important event in the normal development and homeostasis of multicellular organisms (1). Apoptosis is regulated by a number of genes that promote or suppress cell death. The bcl-2 family of genes constitute important regulators of apoptosis. Several members of the bcl-2 family such as bcl-2 (2) and bcl-x L (3) suppress apoptosis, whereas certain other members such as bax (4), bak (5), and bik (6) promote apoptosis Certain animal viruses such as adenovirus, Epstein-Barr virus, herpesvirus simairi, and African swine fever virus also encode structural and functional homologs of Bcl-2. The methods currently available to determine the anti- or pro-apoptotic activities of various cellular and viral Bcl-2 homologs are cumbersome. Here, we describe highly reproducible, simple methods that we have developed to assay the activities of adenovirus E1B 19 kDa, Bcl-2, and various pro-apoptotic interacting partners.


African Swine Fever Virus Antiapoptotic Activity Proapoptotic Gene Suppress Cell Death Early Passage Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Humana Press Inc., Totowa, NJ 1999

Authors and Affiliations

  • T. Subramanian
    • 1
  • G. Chinnadurai
    • 1
  1. 1.Institute for Molecular VirologySt. Louis University School of MedicineSt. Louis

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