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Animal Cell Biotechnology pp 201–209Cite as

Harvesting GPI-Anchored Proteins From CHO Cells

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Part of the book series: Methods in Biotechnology™ ((MIBT,volume 8))

Abstract

Glycosyl-phosphatidylinositol (GPI)-anchored proteins bound to the outer surface of cell membranes (1,2) can be selectively released in a soluble form by the action of a highly specific bacterial enzyme, phosphatidylinositol-phospholipase C (PI-PLC) (3,4). This chapter describes a controlled release process (5–7) that increases both the concentration and purity of harvested GPI-anchored proteins by separating the harvesting of the protein from cell growth and protein expression. This approach has obtained product concentrations of up to 500 μg/mL at purities of 35% total protein (5,7).

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Author information

Authors and Affiliations

  1. Biotechnology Laboratory, University of British Columbia, Vancouver, BC, Canada

    Malcolm L. Kennard

  2. University of British Columbia, Vancouver, BC, Canada

    James M. Piret

Authors
  1. Malcolm L. Kennard
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  2. James M. Piret
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Editor information

Editors and Affiliations

  1. Bioprocess Research and Development, Eli Lilly & Co., Indianapolis, IN

    Nigel Jenkins

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© 1999 Humana Press Inc., Totowa, NJ

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Kennard, M.L., Piret, J.M. (1999). Harvesting GPI-Anchored Proteins From CHO Cells. In: Jenkins, N. (eds) Animal Cell Biotechnology. Methods in Biotechnology™, vol 8. Humana Press. https://doi.org/10.1385/0-89603-547-6:201

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  • DOI: https://doi.org/10.1385/0-89603-547-6:201

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-547-8

  • Online ISBN: 978-1-59259-486-3

  • eBook Packages: Springer Protocols

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