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Adaptation of Mammalian Cells to Growth in Serum-Free Media

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Animal Cell Biotechnology

Part of the book series: Methods in Biotechnology™ ((MIBT,volume 8))

Abstract

The intent of this chapter is to discuss mammalian cell adaptation procedures useful in the development of cell phenotypes compatible with extended cultivation in suspension culture, without serum, and at high cell densities. Such phenotypes are useful for the production of recombinant proteins and monoclonal antibodies for therapeutic or diagnostic applications. Emphasis will be placed on Chinese hamster ovary (CHO) cells due to their widespread use throughout the biotechnology industry. However, the general principles of cell adaptation can be applied to other animal cells, including baby hamster kidney (BHK) and hybridoma cells.

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Author information

Authors and Affiliations

  1. Genetics Institute, Andover, UK

    Martin S. Sinacore, Denis Drapeau & S. R. Adamson

Authors
  1. Martin S. Sinacore
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  2. Denis Drapeau
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  3. S. R. Adamson
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Editor information

Editors and Affiliations

  1. Bioprocess Research and Development, Eli Lilly & Co., Indianapolis, IN

    Nigel Jenkins

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© 1999 Humana Press Inc., Totowa, NJ

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Sinacore, M.S., Drapeau, D., Adamson, S.R. (1999). Adaptation of Mammalian Cells to Growth in Serum-Free Media. In: Jenkins, N. (eds) Animal Cell Biotechnology. Methods in Biotechnology™, vol 8. Humana Press. https://doi.org/10.1385/0-89603-547-6:11

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  • DOI: https://doi.org/10.1385/0-89603-547-6:11

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-547-8

  • Online ISBN: 978-1-59259-486-3

  • eBook Packages: Springer Protocols

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