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Molecular Diagnosis of HIV-1 by PCR

  • Susan A. Fiscus
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 20)

Abstract

Most individuals can be diagnosed as being infected with HIV-1 with an enzyme immunoassay (EIA), which detects antibodies to the virus. Repeatedly positive results must be confirmed, usually by Western blot. These assays have been improved since their introduction in 1985. When used together, the EIA and Western blot are now extremely reliable, with sensitivities of 99.5% and specificities of 99.8% for the diagnosis of HIV-1 (1). These tests are fairly rapid, reproducible across laboratories, well quality-controlled, relatively inexpensive, and form the basis of the protection of the blood supply.

Keywords

Roche Molecular System NASBA Assay Infected Peripheral Blood Mononuclear Cell Copy Number Input Hybridization Protection Assay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 1999

Authors and Affiliations

  • Susan A. Fiscus
    • 1
  1. 1.Department of MedicineUniversity of North Carolina at Chapel HillChapel Hill

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