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Autoradiography of Peptide Receptors

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Receptor Binding Techniques

Part of the book series: Methods in Molecular Biology ((MIMB,volume 106))

Abstract

An important step in understanding receptor function is determining the tissues and cells in which they are expressed. Classical physiological, biochemical, and membrane binding studies, however, usually provide inadequate spatial resolution to determine the anatomical and cellular localisation of specific receptors, particularly where there is a multiplicity of receptor subtypes. Therefore, alternative methods have been developed to provide the required anatomical and cellular resolution, the most widely used of which is in vitro receptor autoradiography. The methods are relatively simple and versatile and are applicable to the analysis of secondary messenger systems, ion channels, and enzymes (see Chapter 11) as well as receptors and may be used in combination with complementary techniques such as immunohistochemistry and in situ hybridization (1). The widespread use of receptor autoradiography reflects the utility of the technique and the advantages it offers over membrane binding studies, in particular the capacity both to localize and quantify ligand binding within heterogeneous tissues without compromising either ligand binding or histological preservation. It is possible to detect and analyze regional differences in the distribution of receptor subtypes within a single tissue sample and thereby derive information that would be difficult, if not impossible, to obtain with other methods.

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Author information

Authors and Affiliations

  1. Department of Histochemistry, Imperial College of Science and Technology, Hamrnersmith Hospital, London, UK

    John Wharton

  2. Rheumatology Unit, University of Nottingham, City Hospital, Nottingham, UK

    David A. Walsh

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  1. John Wharton
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  2. David A. Walsh
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Editors and Affiliations

  1. The Medical School, University of Birmingham, Birmingham, UK

    Mary Keen

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© 1999 Humana Press Inc., Totowa, NJ

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Wharton, J., Walsh, D.A. (1999). Autoradiography of Peptide Receptors. In: Keen, M. (eds) Receptor Binding Techniques. Methods in Molecular Biology, vol 106. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-530-1:99

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  • DOI: https://doi.org/10.1385/0-89603-530-1:99

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-530-0

  • Online ISBN: 978-1-59259-579-2

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