Abstract
The aim of this chapter is to give a general overview of techniques employed in the process of ligand-receptor binding. The theoretical aspects of the ligand-receptor interactions and mathematical aspects of binding are not detailed here. These are considered in Chapter 9, and there are also very readable chapters in refs. 1 and 2. The techniques referred to use radioligands in the study of the central nervous system, but many are applicable to work outside this field, for example, GABA binding in the periphery (3) as well as nonradioligands such as fluorescent and enzyme-linked antibodies.
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References
Hulme, E. C. (ed.) (1992) Receptor-Ligand Interactions, Oxford University Press, New York.
Wharton, J. and Polak, J. M. (eds.) (1993) Receptor Autoradiography Principles and Practice, Oxford University Press, New York.
Erdo, S. L. and Bowery, N. G. (eds.) (1986) GABAergic Mechanisms in the Mammalian Periphery, Raven, New York.
Keen, M. and MacDermot, J. (1993) Analysis of receptors by radioligand binding, in Receptor Autoradiography, Principles and Practice (Wharton, J. and Polak, J. M., eds.), Oxford University Press, New York, pp. 23–55.
Bowery, N. G., Hudson, A. L., and Price, G. W. (1987) GABAA and GABAB receptor site distribution in the rat central nervous system. Neuroscience 20, 365–383.
Baker, J. R. J. (1989) Autoradiography: A Comprehensive Overview, Oxford University Press, New York.
Hudson, A. L. (1993) Autoradiographic techniques, in Receptor Autoradiography, Principles and Practice (Wharton, J. and Polak, J. M., eds.), Oxford University Press, New York, pp. 57–77.
Seeger, T. F. and Pert, A. (1985) Arcuate nucleus stimulation: induction of naloxone reversible analgesia with concomitant localization of opiate release using in vivo autoradiography. Soc. Neurosci. Abs. 15, 1068.
Hulme, E. C. and Buckley, N. J. (1992) Receptor preparations for binding studies, in Receptor-Ligand Interactions (Hulme, E. C., ed.), Oxford University Press, New York, pp. 177–212.
Turner, A. J. and Bachelard, H. S. (eds.) (1987) Neurochemistry: A Practical Approach, IRL, Washington and Oxford, U.K.
Wharton, J., Walsh, D. A., Rutherford, A. D., Knock, G. A., and Polak, J. M. (1993) In vitro autoradiographic localization and characterization of binding sites, in Receptor Autoradiography, Principles and Practice (Wharton, J. and Polak, J. M., eds.), Oxford University Press, New York, pp. 79–102.
McFarthing, K. (1992) Selection and synthesis of receptor-specific radioligands, in Receptor-Ligand Interactions (Hulme, E. C., ed.), Oxford University Press, New York, pp. 1–18.
Bennett, J. P. Jr. and Yamamura, H. I. (1985) Neurotransmitter, hormone, or drug receptor binding methods, in Neurotransmitter Receptor Binding (Yamamura, H. I., Enna, S. J. and Kuhar, M. J., eds.), Raven, New Jersey, pp. 61–89.
Hulme, E. C. and Birdsall, N. J. M. (1992) Strategy and tactics in receptor-binding studies, in Receptor-Ligand Interactions (Hulme, E. C., ed.), Oxford University Press, New York, pp. 63–176.
Slater, P. and Cross, A. J. (1991) Receptors for opiod peptides in brain, in Neuropeptide Technology: Gene Expression and Neuropeptide Receptors. Methods in Neurosciences, vol. 5 (Conn, P. M., ed.), Academic, San Diego, CA, pp. 459–478.
Lowry, O. H. (1951) Protein measurement with Folin’s phenol reagent. J. Biol. Chem. 193, 265–273.
Bradford, M. M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilising the principle of protein-dye binding. Anal. Biochem. 72, 248–254.
Wang, J.-X., Yamamura, H. I., Wang, W., and Roeske, W. R. (1992) The use of the filtration technique in in vitro radioligand binding assays for membrane-bound and solubilized receptors, in Receptor-Ligand Interactions (Hulme, E. C., ed.), Oxford University Press, New York, pp. 213–234.
Hulme, E. C. (1992) Gel-filtration assays for solubilized receptors, in Receptor-Ligand Interactions (Hulme, E. C. ed.), Oxford University Press, New York, pp. 255–263.
Baskin, D. G. (1993) Quantitative analysis of radioligand binding autoradiographs, in Receptor Autoradiography, Principles and Practice (Wharton, J. and Polak, J. M., eds.), Oxford University Press, New York, pp. 107–133.
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© 1999 Humana Press Inc., Totowa, NJ
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Qume, M. (1999). Overview of Ligand-Receptor Binding Techniques. In: Keen, M. (eds) Receptor Binding Techniques. Methods in Molecular Biology, vol 106. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-530-1:3
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DOI: https://doi.org/10.1385/0-89603-530-1:3
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