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Medical Significance of Mycoplasmas

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Mycoplasma Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 104))

Abstract

Mycoplasmas were first isolated from humans in 1937 (1), although it was not until 1951 (2) that selective media were used to identify mycoplasmas in the oropharynx. In 1962, an etiological link was established between what we now know to be Mycoplasma pneumoniae and cases of primary atypical pneumonia (3). Other mycoplasmas have been isolated from predominantly respiratory and genito-urinary sites. These are outlined in Table 1.

Table 1 Mycoplasmas Isolated from Humans
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References

  1. Diens, L. and Edsall, G. (1937) Observations on the L-organism of Klienberger. Proc. Soc. Exp. Biol. Med. 36, 740–744.

    Google Scholar 

  2. Smith, P. F. and Morton, H. E. (1951) Isolation of pleuropneumonia-like organisms from the throat of humans. Science 113, 623,624.

    Google Scholar 

  3. Chanock, R. M., Hayflick, L., and Barile, M. F. (1962) Growth on artificial medium of an agent associated with atypical pneumonia and its identification as a PPLO. Proc. Nat. Acad. Sci. 48, 41–49.

    Article  PubMed  CAS  Google Scholar 

  4. Schaeverbeke, T., Gilroy, C. B., Bebear C., Dehais, J., and Taylor-Robinson, D. (1996) Detection of Mycoplasma fermentans by a PCR assay in synovial fluids from patients with rheumatoid arthritis and other rheumatic disorders. IOM Letters 4, 363.

    Google Scholar 

  5. Reimann, H. A., (1938) An acute infection of the respiratory tract with atypical pneumonia. JAMA 111, 2377–2384.

    Google Scholar 

  6. Eaton, M. D., Meiklejohn, G., and van Herick, W., (1944) Studies on the aetiology of primary atypical pneumonia. A filterable agent transmissible to cotton rats, hamsters and chick embryos. J. Exp. Med. 79, 649–668.

    Article  PubMed  CAS  Google Scholar 

  7. Marmion, B. P. (1990) Eaton agent-science and scientific acceptance: a historical commentary. Rev. Infect. Dis. 12, 338–353.

    PubMed  CAS  Google Scholar 

  8. Marmion, B. P., and Goodburn G. M., (1961) Effect of an inorganic gold salt on Eaton’s primary atypical pneumonia agent and other observations. Nature 189, 247–248.

    Article  PubMed  CAS  Google Scholar 

  9. Chanock, R. M., Dienes, L., Eaton, M. D., Edward, D. G., Freundt, E. A., Hayflick, I., Hers, J. F. P., Jensen, K. E., Liu C., Marmion B. P., Morton H. E., Mufson M. A., Smith P. F., Somerson, N. L., and Taylor-Robinson D. (1963) M. pneumoniae: proposed nomenclature for atypical pneumonia organism (Eaton agent). Science 140, 662.

    Article  PubMed  CAS  Google Scholar 

  10. Staugas, R. and Martin, A. J. (1985) Secondary bacterial infections in children with proved M. pneumoniae. Thorax 40, 546–548.

    Article  PubMed  CAS  Google Scholar 

  11. Hanukoglu, A., Hebroni, S. and Fried, D. (1986) Pulmonary involvement in M. pneumoniae infection in families. Infection 14, 1–6.

    Article  PubMed  CAS  Google Scholar 

  12. Su, C. J., Chavoya, A., Dallo, S. F., and Baseman, J. B. (1990) Sequence divergency of the cytadhesin P1 gene on Mycoplasma pneumoniae. Infect. Immun. 58, 2669–2674.

    PubMed  CAS  Google Scholar 

  13. Goulet, M., Dular, R., Tully, J. G., Billowes, G., and Kasatiya, S. (1995) Isolation of M. pneumoniae from the human urogenital tract. J. Clin. Micro. 33, 2823–2825.

    CAS  Google Scholar 

  14. Niitu, Y., Hasegawa, S., and Kubota, H. (1974) In vitro development of resistance to erythromycin, other macrolide antibiotics and lincomycin in M. pneumoniae. Antimicrob. Agents Chemother. 5, 513–518.

    CAS  Google Scholar 

  15. Tully, J. G., Taylor-Robinson, D., Cole, R. M., and Rose, D. L. (1981) A newly discovered mycoplasma in the human urogenital tract. Lancet 1,1228–1291.

    Google Scholar 

  16. Baseman, J. B., Dallo, S. F., Tully, J. G., and Rose, D. L., (1988) Isolation and characterisation of Mycoplasma genitalium strains from the respiratory tract. J. Clin. Microbiol. 26, 2266–2269.

    PubMed  CAS  Google Scholar 

  17. Palmer, H. M., Gilroy, C. B., Claydon, E. J., and Taylor-Robinson, D. (1991) Detection of Mycoplasma genitalium in the genitourinary tract of women by the polymerase chain reaction. Int. J. STD AIDS 2, 261–263.

    PubMed  CAS  Google Scholar 

  18. Jensen, J. S., Orsum, R., Dohn, B., Uldum, S., Wormm A. M., and Lind, K. (1993) Mycoplasma genitalium: a cause of male urethritis? Genitourin. Med. 69, 265–269.

    PubMed  CAS  Google Scholar 

  19. Berbeyrac, B., Bernet-Poggi, C., Febrer, F., Renaudin, H., Dupon, M., and Bebear, C. (1993) Detection of M. pneumoniae and M. genitalium in clinical samples by polymerase chain reaction. Clin. Infect. Dis. 17 (Suppl. 1), S83–89.

    Google Scholar 

  20. Taylor-Robinson, D., Gilroy, C. B., Horowitz, S., and Horowitz, J. (1994) Mycoplasma genitalium in the joints of two patients with arthritis. Eur. J. Clin. Micro. Inf. Dis. 13, 1066–1069.

    Article  CAS  Google Scholar 

  21. Renaudin, H., Tully, J. G., and Bebear, C. (1992) In vitro susceptibilitis of Mycoplasma genitalium to antibiotics. Antimicrob. Agents Chemother. 36, 870–872.

    PubMed  CAS  Google Scholar 

  22. Shepard, M. C., (1954) The recovery of pleuropneumonia-like organisms from Negro men with and without non-gonococcal urethritis. Am. J. Syph. Gonorrhoea Vener. Dis. 38, 113–124.

    CAS  Google Scholar 

  23. Shepard, M. C., Lunceford, C. D., Ford, D. K., Purcell, R. H., Taylor-Robinson D., Razin, S., and Black, F. T. (1974) Ureaplasma urealyticum gen. nov., sp. Nov: proposed nomenclature for the human T (T-strain) mycoplasma. Int. J. Syst. Bacteriol. 24, 160–171.

    Article  Google Scholar 

  24. Taylor-Robinson, D. and McCormack, W. M. (1980) The genital mycoplasmas. N. Engl. J. Med. 302, 1003–1008.

    Article  PubMed  CAS  Google Scholar 

  25. Cassell, G. H., Waites, K. B., Watson, H. I., Crouse, D. T., and Harasawa, R. (1993) Ureaplasma urealyticum intrauterine infection: role in prematurity and disease in newborns. Clin. Micro. Rev. 6, 69–87.

    PubMed  CAS  Google Scholar 

  26. Taylor-Robinson, D., Furr, P., and Webster, A. D. B. (1986) Ureaplasma urealyticum in the immunocompromised host. Paediatr. Infect. Dis. 5 (Suppl.), 236–238.

    Article  Google Scholar 

  27. Gray, D. J., Robinson, H. B., Malone, J., and Thompson, R. B. (1991) Adverse outcome in pregnancy following amniotic fluid isolation of Ureaplasma urealyticum. Prenat. Diagn. 12, 111–117.

    Article  Google Scholar 

  28. Cassell, G. H., Waites, K. B., Watson, H. L., Crouse, D. T., and Harawasa R. (1993) Ureaplasma urealyticum intrauerine infection: role in prematurity and disease in new-borns. Clin. Microbiol Rev. 6, 69–87.

    PubMed  CAS  Google Scholar 

  29. Simson, J. B., Hale, J., Bowie, W. R., and Holmes, K. K. (1981) Tetracycline resistant Ureaplasma urealyticum: a cause of persistent nongonococcal urethritis. Ann. Intern. Med. 94, 192–194.

    Google Scholar 

  30. Mardh, P.-A. and Westrom, L. (1970) Tubal and cervical cultures in acute salpin-gitis with special reference to Mycoplasma hominis and T-strain mycoplasmas. Br. J. Vener. Dis. 46, 179–186.

    PubMed  CAS  Google Scholar 

  31. Taylor-Robinson, D., and Munday, P. E. (1988) Mycoplasmal infections of the female genital tract and its complications, in Genital Tract Infection in Women (Hare, M. J., ed.) Churchill Livingstone, Edinburgh, pp. 228–247.

    Google Scholar 

  32. Thomsen, A. C., and Lindskov, H. O. (1979) Diagnosis of Mycoplasma hominis pyelonephritis by demonstration of antibodies in urine. J. Clin. Microbiol. 9, 681–687.

    PubMed  CAS  Google Scholar 

  33. Sielaff, T. D., Everett, J. E., Shumway, S. J., Wahoff, D. C., Bolman, R. M. and Dunn, D. L. (1996) Mycoplasma hominis infections occurring in cardiovascular surgical patients. Ann. Thorac. Surg. 61, 99–103.

    Article  PubMed  CAS  Google Scholar 

  34. Luttrell, L. M., Kanj, S. S., Corey, G. R., Lins, R. E., Spinner, R. J., Mallon, W. J., and Sexton, D. J. (1994) Mycoplasma hominis septic arthritis: two case reports and review. Clin. Inf. Dis 19, 1067–1070.

    CAS  Google Scholar 

  35. McCormack, W. M., Lee, Y-H., and Zinner, S. H. (1973) Sexual activity and vaginal colonisation with genital mycoplasmas. Ann. Intern. Med. 78, 696–698.

    PubMed  CAS  Google Scholar 

  36. Cummings, M. C. and McCormack, W. M. (1990) Increase in resistance of Mycoplasma hominis to tetracyclines. Antimicrob. Agents Chemother. 34, 2297–2299.

    PubMed  CAS  Google Scholar 

  37. Teel, L. D., Fineli, M. R., and Johnson, S. C. (1994) Isolation of mycoplasma species from bronchoalveolar lavages of patients positive and negative for human immunodeficiency virus. J. Clin. Micro. 32, 1387–1389.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Virology Department, Royal Brompton National Heart and Lung Hospital, London, UK

    Paul Taylor

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  1. Paul Taylor
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Editors and Affiliations

  1. King’s College, London, England

    Roger Miles

  2. Central Veterinary Laboratory, Surrey, England

    Robin Nicholas

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© 1998 Humana Press Inc., Totowa, NJ

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Taylor, P. (1998). Medical Significance of Mycoplasmas. In: Miles, R., Nicholas, R. (eds) Mycoplasma Protocols. Methods in Molecular Biology™, vol 104. Humana Press. https://doi.org/10.1385/0-89603-525-5:7

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  • DOI: https://doi.org/10.1385/0-89603-525-5:7

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-525-6

  • Online ISBN: 978-1-59259-269-2

  • eBook Packages: Springer Protocols

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