Abstract
The polymerase chain reaction (PCR), DNA hybridization, and sequence analysis have been valuable in the study of the phylogenic relationships between members of the Mycoplasma mycoides “cluster” (1). They have confirmed the very close relationships between these organisms suggested by others (2,3), and in the case of M. mycoides subsp. mycoides SC (MmmSC), provided a rapid diagnostic test for the detection and identification of this organism based on DNA amplification by PCR and restriction fragment length polymorphism (RFLP) between MmmSC and (MmmLC) (4).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Taylor, T. K., Bashiruddin, J. B., and Gould, A. R. (1992) Relationships between the members of the Mycoplasma mycoides cluster as shown by DNA probes and sequence analysis. Int. J. System. Bacteriol. 42, 593–601.
Askaa, G., ErnØ, H., and Ojo, M. O. (1978) Bovine mycoplasmas: classification of groups related to Mycoplasma mycoides. Acta. Vet. Sand. 19, 166–178.
Costas, M., Leach, R. H., and Mitchelmore, D. L. (1987) Numerical analysis of PAGE protein patterns and the taxonomic relationships within the “Mycoplasma mycoides cluster”. J. Gen. Microbiol. 133, 3319–3329.
Bashiruddin, J. B., Taylor, T. K., and Gould, A. R. (1994) A PCR based test for the specific identification of Mycoplasma mycoides subspecies mycoides SC. J. Vet. Diagn. Invest. 6, 428–434.
Egwu, M. O., Nicholas, R. A. J., Ameh, J. A., and Bashiruddin, J. B. (1996) Contagious bovine pleuropneumonia: an update. Vet. Bull. 66, 875–888.
Nicholas, R. A. J. and Bashiruddin, J. B. (1996) Mycoplasma mycoides subspecies mycoides (small colony variant): the agent of contagious bovine pleuropneumonia and a member of the “Mycoplasma mycoides cluster”. J. Comp. Pathol. 113, 1–27.
Bashiruddin, J. B., Nicholas, R. A. J., Santini, F. G., Woodward, M. J., and Taylor, T. K. (1994). Use of Polymerase chain reaction to detect mycoplasma DNA in cattle with contagious bovine pleuropneumonia. Vet. Rec. 134, 240–241.
Brandão, E. (1995) Isolation and identification of Mycoplasma mycoides subsp. mycoides SC strains in sheep and goats. Vet. Rec. 136, 98–99.
Brandão, E., Botelho, A., Bashiruddin, J. B., and Nicholas, R. A. J. (1994) Use of polymerase chain reaction for identification of Mycoplasma mycoides subsp. mycoides SC isolated from sheep and goats. IOM Letters 3, 24–25.
Dedieu, L., Mady, V., and Lefevre, P. C. (1994) Development of a selective polymerase chain reaction assay for the detection of Mycoplasma mycoides subsp. mycoides SC (contagious bovine pleuropneumonia agent). Vet. Microbiol. 42, 327–339.
De Santis, P. and Bashiruddin, J. B. (1996) Application of Mycoplasma mycoides subsp. mycoides PCR to Italian field samples, in Mycoplasmas of Ruminants: Pathogenicity, Diagnostics, Epidemiology and Molecular Genetics (Frey, J. and Sarris, K., eds.), EUR 16394 EC, Brussels, pp. 141–143.
Kaura, H. T. and Hübschle, O. J. B. (1996) Use of PCR to detect Mycoplasma mycoides subspecies mycoides from nasal filter strips. Vet. Rec. 138, 444–445.
Kemp, D. J., Smith, D. B., Foote, S. J., Samaras, N., and Peterson, M. G. (1989) Colorimetric detection of specific DNA segments amplified by polymerase chain reactions. Proc. Natl. Acad. Sci. USA 86, 2423–2427.
Katz, E. D. (1993) Quantitation and purification of polymerase chain reaction products by high-performance liquid chromatography, in PCR Protocols: Current Methods and Applications, vol. 15 (White, B., ed.), Humana Press, Totowa, NJ, pp. 63–74.
Lazar, J. G. (1996) Immunological detection of PCR products, in PCR Primer: A Laboratory Manual (Dieffenbach, C. W. and Dveksler, G. S., eds.), pp. 177–192.
Dieffenbach, C. W., Dragon, E. A., and Dveksler, G. S. (1996) Setting up a PCR Laboratory, in PCR Primer: A Laboratory Manual (Dieffenbach, C. W. and Dveksler, G. S., eds.), Cold Spring Harbor Laboratory Press, NY, pp. 7–16.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Bashiruddin, J.B. (1998). PCR and RFLP Methods for the Specific Detection and Identification of Mycoplasma mycoides subsp. mycoides SC. In: Miles, R., Nicholas, R. (eds) Mycoplasma Protocols. Methods in Molecular Biology™, vol 104. Humana Press. https://doi.org/10.1385/0-89603-525-5:167
Download citation
DOI: https://doi.org/10.1385/0-89603-525-5:167
Publisher Name: Humana Press
Print ISBN: 978-0-89603-525-6
Online ISBN: 978-1-59259-269-2
eBook Packages: Springer Protocols