Abstract
The diagnosis and monitoring of hepatitis C virus (HCV) infection have been aided by the development of HCV RNA quantification assays A direct measure of viral load, HCV RNA quantification has the advantage of providing information on viral kinetics and provides unique insight into the disease process. Branched DNA (bDNA) signal amplification technology provides a novel approach for the direct quantification of HCV RNA in patient specimens. The bDNA assay measures HCV RNA at physiological levels by boosting the reporter signal, rather than by replicating target sequences as the means of detection, and thus avoids the errors inherent in the extraction and amplification of target sequences. Inherently quantitative and nonradioactive, the bDNA assay is amenable to routine use in a clinical research setting, and has been used by several groups to explore the natural history, pathogenesis, and treatment of HCV infection
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Ā© 1998 Humana Press Inc., Totowa, NJ
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Wilber, J.C., Urdea, M.S. (1998). Quantification of HCV RNA in Clinical Specimens by Branched DNA (bDNA) Technology. In: Lau, J.YN. (eds) Hepatitis C Protocols. Methods in Molecular Medicineā¢, vol 19. Humana Press. https://doi.org/10.1385/0-89603-521-2:71
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DOI: https://doi.org/10.1385/0-89603-521-2:71
Publisher Name: Humana Press
Print ISBN: 978-0-89603-521-8
Online ISBN: 978-1-59259-260-9
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