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Replication and Detection of Hepatitis C Virus in Liver-Derived Cell Lines

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Book cover Hepatitis C Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 19))

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Abstract

A model of HCV replication has several potential important applications (Table 1). In addition to humans, the natural host for HCV, chimpanzees are the only other animals that have been shown to be permissive to HCV infection. However, the primate model for HCV infection presents several problems, namely, limited availability, cost, and different host response to HCV compared to humans.

Table 1 Potential Applications of a Model of HCV Replication
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References

  1. Iacovacci, S., Sargiacomo, M., Parolini, I., Ponzetto, A, Peschle, C, and Carloni, G (1993) Replication and multiplication of hepatitis C virus in human fetal liver cells. Res Virol 144, 275–279.

    Article  PubMed  CAS  Google Scholar 

  2. Lanford, R. E, Sureau, C, Jacob, J. R, White, R., and Fuerst, T. R. (1994) Demonstration of in vitro infection of chimpanzee hepatocytes with hepatitis C virus using strand-specific RT/PCR Virology 202, 606–614.

    Article  PubMed  CAS  Google Scholar 

  3. Ito, T., Mukaigawa, J, Zuo, J., Hirabayashi, Y., Mitamura, K, and Yasui, K (1996) Cultivation of hepatitis C virus in primary hepatocyte culture from patients with chronic hepatitis C virus results in release of high titer infectious virus. J Gen Virol 11, 1043–1054

    Article  Google Scholar 

  4. Kato, N, Ikeda, M, Mizutani, T, Sugiyama, K, Noguchi, M., Hirohashi, S., et al (1996) Replication of hu atitis C virus in cultured non-neoplastic human hepatocytes Jpn. J Cancer Res. 87, 787–792.

    PubMed  CAS  Google Scholar 

  5. Shimizu, Y K, Iwamoto, A., Hijikata, M, Purcell, R H, and Yoshikura, H. (1992) Evidence for in vitro replication of hepatitis C virus genome in a human T-cell line. Proc Natl. Acad. Sci. USA 89, 5477–5481

    Article  PubMed  CAS  Google Scholar 

  6. Shimizu, Y K. and Yoshikura, H. (1994) Multicycle of infection of hepatitis C virus in cell culture and inhibition by alpha and beta interferons. J Virol 68, 8406–8408

    PubMed  CAS  Google Scholar 

  7. Mizutani, T, Kato, N, Saito, S, Ikeda, M, Sugiyama, K., and Shimotohno, K. (1996) Characterization of hepatitis C virus replication in cloned cells obtained from a human T-cell leukemia virus type 1-infected cell line, MT-2 J. Virol 70, 7219–7223.

    PubMed  CAS  Google Scholar 

  8. Gonzälez-Peralta, R. P, Liu, W, and Lau, J Y N (1996) Identification of liver-derived cell lines that support low-level hepatitis C virus replication [Abstract] Hepatology 24, 217A

    Article  Google Scholar 

  9. McGuinness, P H, Bishop, G A, McCaughan, G W, Trowbridge, R, and Gowans, E. J. (1994) False detection of negative-strand hepatitis C virus RNA. Lancet 343, 551,552.

    Article  PubMed  CAS  Google Scholar 

  10. Lanford, R E., Chavez, D, Chisari, F V., and Sureau, C. (1996) Lack of detection of negative-strand hepatitis C virus RNA in peripheral blood mononuclear cells and other extrahepatic tissues by the highly strand-specific rTth reverse transcriptase PCR. J Virol. 69, 8079–8083.

    Google Scholar 

  11. Davis, G L, Lau, JYN, Urdea, M S, Neuwald, P, Wilber, J. C, Lindsay, K, et al (1994) Quantitative detection of hepatitis C virus RNA by solid phase branched DNA amplification method, definition and optimal conditions for specimen collection and clinical application in interferon-treated patients. Hepatology 19, 1337–1341.

    Article  PubMed  CAS  Google Scholar 

  12. Lau, J. Y N, Mizokami, M., Kolberg, J. A, Davis, G. L., Prescott, L. E., Ohno, T., et al. (1995) Application of six hepatitis C virus genotyping systems to sera from chronic hepatitis C patients in the United States J Infect. Dis. 171, 281–289

    PubMed  CAS  Google Scholar 

  13. Yanagi, M., Bukh, J., Emerson, S. U., and Purcell, R. H. (1996) Contamination of commercially available fetal bovine sera with bovine diarrhea virus: implications for the study of hepatitis C virus in cell cultures. J Infect Dis, in press

    Google Scholar 

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Author information

Authors and Affiliations

  1. Division of Gastroenterology and Hepatology, Department of Pediatrics, University of Florida, Gainesville, FL

    Regino P. Gonzáalez-Peralta

  2. Section of Hepatobiliary Diseases, Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, University of Florida, Gainesville, FL

    Regino P. Gonzáalez-Peralta

Authors
  1. Regino P. Gonzáalez-Peralta
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Editor information

Editors and Affiliations

  1. ICN Pharmaceuticals, Costa Mesa, CA

    Johnson Yiu-Nam Lau MD

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© 1998 Humana Press Inc., Totowa, NJ

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Gonzáalez-Peralta, R.P. (1998). Replication and Detection of Hepatitis C Virus in Liver-Derived Cell Lines. In: Lau, J.YN. (eds) Hepatitis C Protocols. Methods in Molecular Medicine™, vol 19. Humana Press. https://doi.org/10.1385/0-89603-521-2:489

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  • DOI: https://doi.org/10.1385/0-89603-521-2:489

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-521-8

  • Online ISBN: 978-1-59259-260-9

  • eBook Packages: Springer Protocols

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