Abstract
A model of HCV replication has several potential important applications (Table 1). In addition to humans, the natural host for HCV, chimpanzees are the only other animals that have been shown to be permissive to HCV infection. However, the primate model for HCV infection presents several problems, namely, limited availability, cost, and different host response to HCV compared to humans.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Iacovacci, S., Sargiacomo, M., Parolini, I., Ponzetto, A, Peschle, C, and Carloni, G (1993) Replication and multiplication of hepatitis C virus in human fetal liver cells. Res Virol 144, 275–279.
Lanford, R. E, Sureau, C, Jacob, J. R, White, R., and Fuerst, T. R. (1994) Demonstration of in vitro infection of chimpanzee hepatocytes with hepatitis C virus using strand-specific RT/PCR Virology 202, 606–614.
Ito, T., Mukaigawa, J, Zuo, J., Hirabayashi, Y., Mitamura, K, and Yasui, K (1996) Cultivation of hepatitis C virus in primary hepatocyte culture from patients with chronic hepatitis C virus results in release of high titer infectious virus. J Gen Virol 11, 1043–1054
Kato, N, Ikeda, M, Mizutani, T, Sugiyama, K, Noguchi, M., Hirohashi, S., et al (1996) Replication of hu atitis C virus in cultured non-neoplastic human hepatocytes Jpn. J Cancer Res. 87, 787–792.
Shimizu, Y K, Iwamoto, A., Hijikata, M, Purcell, R H, and Yoshikura, H. (1992) Evidence for in vitro replication of hepatitis C virus genome in a human T-cell line. Proc Natl. Acad. Sci. USA 89, 5477–5481
Shimizu, Y K. and Yoshikura, H. (1994) Multicycle of infection of hepatitis C virus in cell culture and inhibition by alpha and beta interferons. J Virol 68, 8406–8408
Mizutani, T, Kato, N, Saito, S, Ikeda, M, Sugiyama, K., and Shimotohno, K. (1996) Characterization of hepatitis C virus replication in cloned cells obtained from a human T-cell leukemia virus type 1-infected cell line, MT-2 J. Virol 70, 7219–7223.
Gonzälez-Peralta, R. P, Liu, W, and Lau, J Y N (1996) Identification of liver-derived cell lines that support low-level hepatitis C virus replication [Abstract] Hepatology 24, 217A
McGuinness, P H, Bishop, G A, McCaughan, G W, Trowbridge, R, and Gowans, E. J. (1994) False detection of negative-strand hepatitis C virus RNA. Lancet 343, 551,552.
Lanford, R E., Chavez, D, Chisari, F V., and Sureau, C. (1996) Lack of detection of negative-strand hepatitis C virus RNA in peripheral blood mononuclear cells and other extrahepatic tissues by the highly strand-specific rTth reverse transcriptase PCR. J Virol. 69, 8079–8083.
Davis, G L, Lau, JYN, Urdea, M S, Neuwald, P, Wilber, J. C, Lindsay, K, et al (1994) Quantitative detection of hepatitis C virus RNA by solid phase branched DNA amplification method, definition and optimal conditions for specimen collection and clinical application in interferon-treated patients. Hepatology 19, 1337–1341.
Lau, J. Y N, Mizokami, M., Kolberg, J. A, Davis, G. L., Prescott, L. E., Ohno, T., et al. (1995) Application of six hepatitis C virus genotyping systems to sera from chronic hepatitis C patients in the United States J Infect. Dis. 171, 281–289
Yanagi, M., Bukh, J., Emerson, S. U., and Purcell, R. H. (1996) Contamination of commercially available fetal bovine sera with bovine diarrhea virus: implications for the study of hepatitis C virus in cell cultures. J Infect Dis, in press
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Gonzáalez-Peralta, R.P. (1998). Replication and Detection of Hepatitis C Virus in Liver-Derived Cell Lines. In: Lau, J.YN. (eds) Hepatitis C Protocols. Methods in Molecular Medicine™, vol 19. Humana Press. https://doi.org/10.1385/0-89603-521-2:489
Download citation
DOI: https://doi.org/10.1385/0-89603-521-2:489
Publisher Name: Humana Press
Print ISBN: 978-0-89603-521-8
Online ISBN: 978-1-59259-260-9
eBook Packages: Springer Protocols