Skip to main content

Detection and Molecular Cloning of the Extreme 3′-End of HCV

  • Protocol
  • 2256 Accesses

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 19))

Abstract

The 3′-end region of the HCV genome is composed of three characteristic elements: conventional 3′-untranslated region, subsequent poly (U) stretch, and a newly identified 3′-terminal sequence named the 3′X tail (1,2). The 3′X tail is thought to be a common structure of the HCV genome, and should have important roles in initiation and regulation of the genomic replication of HCV.

This is a preview of subscription content, log in via an institution.

Buying options

eBook
USD   39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Tanaka, T., Kato, N., Cho, M.-J., and Shimothono, K. (1995) A novel sequence found at the 3′ terminus of hepatitis C virus genome. Biochem Biophys Res Commun 215, 744–749.

    Article  PubMed  CAS  Google Scholar 

  2. Tanaka, T., Kato, N., Cho, M.-J, Sugiyama, K., and Shimotohno, K. (1996) Structure of the 3′ terminus of the hepatitis C virus genome. J. Virol 70, 3307–3312

    PubMed  CAS  Google Scholar 

  3. Kolykhalov, A A., Feinstone, S. M., and Rice, C. M. (1996) Identification of a highly conserved sequence element at the 3′ terminus of hepatitis C virus genome RNA J Virol. 70, 3363–3371

    PubMed  CAS  Google Scholar 

  4. Yamada, N., Tanihara, K., Takada, A., Yorihuzi, T., Tsutsumi, M., Shimomura, H., et al. (1996) Genetic organization and diversity of the 3′ noncoding region of the hepatitis C virus genome Virology 223, 255–261.

    Article  PubMed  CAS  Google Scholar 

  5. Sambrook, J., Fritsch, E F, and Maniatis, T. (1989) Molecular Cloning, A Laboratory Manual Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1998 Humana Press Inc., Totowa, NJ

About this protocol

Cite this protocol

Tanakaand, T., Shimotohno, K. (1998). Detection and Molecular Cloning of the Extreme 3′-End of HCV. In: Lau, J.YN. (eds) Hepatitis C Protocols. Methods in Molecular Medicine™, vol 19. Humana Press. https://doi.org/10.1385/0-89603-521-2:373

Download citation

  • DOI: https://doi.org/10.1385/0-89603-521-2:373

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-521-8

  • Online ISBN: 978-1-59259-260-9

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics