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HCV Genotyping by the Line Probe Assay INNO-LiPA HCV II

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Hepatitis C Protocols

Part of the book series: Methods in Molecular Medicineā„¢ ((MIMM,volume 19))

Abstract

Hepatitis C viruses (HCVs) constitute a highly variable genus within the Flaviviridae, with closest homology to the hepatitis G and GB viruses, and Pestiviruses. The positive-stranded RNA genome encodes a polyprotein which is co- and posttranslationally cleaved into at least nine proteins. Core, E1, and E2 (the structural proteins) and NS2, NS3, NS4A, NS4B, NS5A, and NS5B (the nonstructural [NS] proteins). A theoretical protein of 7 kDa (tp7) may be processed from the carboxy terminal E2 region (1).

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References

  1. Maertens, G. and Stuyver, L. (1997) Genotypes and Genetic variation of hepatitis C virus, in Molecular Medicine of Viral Hepatitis (Zuckerman, A. and Harrison, T, eds.), Molecular Medical Science Series (James, K and Morris A, eds), John Wiley, Chichester, England, pp. 183ā€“233

    Google ScholarĀ 

  2. Hoofnagle, J and Di Bisceglie, A. (1997) The treatment of chronic viral hepatitis N Engl J Med. 336, 347ā€“356

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  3. Chemello, L, Bonetti, P, Cavalletto, L, Talato, F., Donadon, V., Casarin, P, et al (1995) Randomized trial comparing three different regimens of alpha-2a-interferon in chronic hepatitis C Hepatology 22, 700ā€“706

    PubMedĀ  CASĀ  Google ScholarĀ 

  4. Qu, D., Li, J-S, Vivitski, L., Mechai, S., Berby, F, Tong, S-P, et al (1994) Hepatitis C virus genotypes in France: comparison of clinical features of patients infected with HCV type I and type II. J Hepatol 21, 70ā€“75

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  5. Tsubota, A., Chayama, K, Ikeda, K., Yasuji, A., Koida, I., Saitoh, S, et al. (1994) Factors predictive of response to interferon-alfa therapy in hepatitis C infection. Hepatology 19, 1088ā€“1094.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  6. Nousbaum, J-B, Pol, S, Nalpas, B., Landais, P., Berthelot, P., and BrĆ©chot, C (1995) Hepatitis C virus type lb (II) infection in France and Italy Ann Intern Med 111, 161ā€“168

    Google ScholarĀ 

  7. El-Zayadi, A., Selim, O, El Haddad, S, and Hamdy, H. (1995) Combination treatment of alpha interferon-2b and Ribavirin in chronic hepatitis C genotype 4 patients resistant to interferon therapy [Abstract]. Hepatology 22 (Pt. 2), 152A

    ArticleĀ  Google ScholarĀ 

  8. Maggi, F., Vatteroni, M, Fornai, C, Morrica, A, Giorgi, M, Bendinelli, M., et al. (1997) Subtype 2c hepatitis C virus is highly prevalent in Italy and is heterogeneous in the NS5A region. J Clin. Microbiol 35, 161ā€“164

    PubMedĀ  CASĀ  Google ScholarĀ 

  9. Okamoto, H., Crovatto, M, Pozzato, G., FĆ©ray, C, BrĆ©chot, C, and Mishiro, S (1995) Molecular and clinical characteristics of the hepatitis C virus genotype 2c found in Italians in Italy and France Int Hepatol Commun 3, 161ā€“165

    ArticleĀ  Google ScholarĀ 

  10. Chemello, L, Cavaletto, L., Bernardinello, E, Guido, M, Pontisso, P., and Alberti, A (1995) The effect of interferon alfa and ribavirin combination therapy in naive patients with chronic hepatitis C. J. Hepatol. 23 (Suppl. 2), 8ā€“12.

    PubMedĀ  CASĀ  Google ScholarĀ 

  11. Gane, E., Naoumov, N., Qian, K.-P, Mondelli, M, Maertens, G, Portmann, B, et al. (1996) A longitudinal analysis of hepatitis C virus replication following liver transplantation. Gastroenterology 110, 167ā€“177.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  12. Gane, E, Portmann, B, Naoumov, N., Smith, H., Underhill, J., Donaldson, P., et al. (1996) Long-term outcome of hepatitis C infection after liver transplantation. N Engl J Med. 334, 815ā€“820.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  13. FĆ©ray, C, Gigou, M., Samuel, D., Paradis, V., Mishiro, S., Maertens, G, ReynĆØs, M, et al. (1995) Influence of the genotypes of hepatitis C virus on the severity of recurrent liver disease after liver transplantation. Gastroenterology 108, 1088ā€“1096

    ArticleĀ  PubMedĀ  Google ScholarĀ 

  14. Di Martino, V, FĆ©ray, C, Saurini, F, Samuel, D, ReynĆØs, M, and Bismuth, H (1995) Relation between intra-hepatic replication, genotypes and recurrent liver disease due to hepatitis C virus after liver transplantation [Abstract]. J Hepatol. 23 (Suppl. 1), 152.

    Google ScholarĀ 

  15. Chant, K., Kociuba, K., Munro, R., Crone, S., Kerrdige, R., Quin, J., et al (1994) Investigation of possible patient-to-patient transmission of hepatitis C in a hospital New South Wales Heath Bull 5, 47ā€“51.

    ArticleĀ  Google ScholarĀ 

  16. Zignego, A., Ferri, C, Giannini, C, Monti, M., La Civita, L, Careccia, G., et al (1996) Hepatitis C virus genotype analysis in patients with type II mixed cryoglobulinemia Ann Intern Med 124, 31ā€“34.

    PubMedĀ  CASĀ  Google ScholarĀ 

  17. Kanazawa, Y., Hayashi, N., Mita, E., Li, T., Hagiwara, H, Kasahara, A., et al (1994) Influence of viral quasispecies on effectiveness of interferon therapy in chronic hepatitis C patients. Hepatology 20, 1121ā€“1130.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  18. Honda, M, Kaneko, S, Sakai, A., Unoura, M., Murakami, S., and Kobayashi, K (1994) Degree of hepatitis C virus quasispecies and progression to liver disease Hepatology 20, 1144ā€“1151.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  19. Allander, T, Medin, C, Jacobson, S., Grillner, L., and Persson, M. (1994) Hepatitis C transmission in a hemodialysis unit: molecular evidence for spread of virus among patients not sharing equipment. J. Med Virol 43, 514ā€“419

    ArticleĀ  Google ScholarĀ 

  20. Simmonds, P., Alberti, A., Alter, H., Bonino, F., Bradley, D.W., BrĆ©chot, C., et al (1994) A proposed system for the nomenclature of hepatitis C virus genotypes Hepatology 19, 1321ā€“1324.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  21. Stuyver, L., van Arnhem, W, Wyseur, A., Hernandez, F, Delaporte, E, and Maertens, G (1994) Classification of hepatitis C viruses based on phylogenetics analysis of the E1 and NS5B regions and identification of 5 new subtypes. Proc Natl Acad Sci USA 91, 10,134ā€“10,138.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  22. Tokita, H., Okamoto, H., Tsuda, F, Song, P, Nakata, S, Chosa, T., et al (1994) Hepatitis C virus variants from Vietnam are classifiable into the seventh, eighth, and ninth major genetic groups. Proc Natl Acad Sci USA 91, 11,022ā€“11,026

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  23. Stuyver, L., Wyseur, A, van Arnhem, W., Hernandez, F, and Maertens, G (1996) A second generation INNO-LiPA for hepatitis C virus genotyping. J. Clin Microbiol 34 (9), 2259ā€“2266.

    PubMedĀ  CASĀ  Google ScholarĀ 

  24. Stuyver, L., Rossau, R., Wyseur, A., Duhamel, M., Vanderborght, B., Van Heuverswyn, H., et al. (1993) Typing of hepatitis C virus isolates and characterization of new subtypes using a line probe assay. J Gen. Virol 74, 1093ā€“1102.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  25. Stuyver, L, Wyseur, A., Rombout, A, Louwagie, J, Scarcez, T, Verhofstede, C, et al (1997) Line probe assay for rapid detection of drug-selected mutations in the human immunodeficiency virus type 1 reverse transcriptase gene Antimicrob Agents Chemother. 41, 284ā€“291

    PubMedĀ  CASĀ  Google ScholarĀ 

  26. Stuyver, L., Rossau, R., and Maertens, G. (1996) Line probe assays for the detection of HCV and HBV genotypes Antiviral Ther. 1 (Suppl. 3), 53ā€“57

    CASĀ  Google ScholarĀ 

  27. Smith, D., Mellor, J, Jarvis, L, Davidson, F., Kolberg, J, Urdea, M., et al (1995) Variation of the hepatitis C virus 5ā€² non-coding region. implications for secondary structure, virus detection and typing. J Gen. Virol 76, 1749ā€“1761.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  28. van Doorn, L.-J., Kleter, B., Stuyver, L., Maertens, G., Brouwer, J., Schalm, S, et al. (1994) Analysis of hepatitis C virus genotypes by a line probe assay (LiPA) and correlation with antibody profiles J Hepatol 12, 122ā€“129

    Google ScholarĀ 

  29. Mahaney, K., Tedeschi, V., Maertens, G., Di Bisceglie, A, Vergalla, J., Hoofnagle, J., et al. (1994) Genotypic analysis of hepatitis C virus in American patients. Hepatology 20, 1405ā€“1411.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  30. Gianmni, C, Thiers, V, Nousbaum, J.-B, Stuyver, L., Maertens, G, and BrĆ©chot, C (1994) Comparative analysis of the hepatitis C virus core PCR and LiPA genotyping assays J Hepatol 23, 246ā€“253.

    Google ScholarĀ 

  31. Zeuzem, S., Ruster, B., Lee, J.-H., Stripf, T, and Roth, K. (1995) Evaluation of a reverse hybridization assay for genotyping of hepatitis C virus J Hepatol 23, 654ā€“661.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  32. Stuyver, L, Fretz, C, Esquivel, C, Boudifa, A, Jaulmes, D., Azar, N, et al (1996) HCV genotype analysis in apparantly healthy anti-HCV positive Parisian blood donors Transfusion 36, 552ā€“558.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  33. Stuyver, L., Wyseur, A., van Arnhem, W, Lunel, F., Laurent-Puig, P., Pawlotsky J.-M., et al. (1995) Hepatitis C virus genotyping by means of 5ā€²UR/Core line probe assays and molecular analysis of untypeable samples Virus Res 38, 137ā€“157

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  34. Mellor, J., Holmes, E., Jarvis, L., Yap, P.-L, and Simmonds, P (1995) Investigation of the pattern of hepatitis C virus sequence diversity in different geographical regionsā€™ implications for virus classification J Gen Virol 76, 2493ā€“2507

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  35. Okamoto, H., Sugiyama, Y, Okada, S., Kurai, K, Akahane, Y., Sugai, Y, et al, (1992) Typing of hepatitis C virus by polymerase chain raction with type-specific primersā€™ application to clinical surveys and tracing infectious sources J Gen. Virol 73, 673ā€“679

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  36. Cammarota, G, Maggi, F, Vatteroni, M., Da Prato, M., Barsanti, L, Bendinelli, M., et al. (1995) Partial nucleotide sequencing of six subtype 2c hepatitis C viruses detected in Italy J Clin Microbiol 33, 2781ā€“2784

    PubMedĀ  CASĀ  Google ScholarĀ 

  37. Kleter, G, van Doorn, L.-J, Stuyver, L., Maertens, G., Brouwer, J, Schalm, S, et al. (1995) Rapid genotyping of hepatitis C virus RNA-isolates obtained from patients residing in Western Europe J. Med Virol. 47, 35ā€“42.

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  38. Andonov, A. and Chaudary, R. (1994) Genotyping of Canadian hepatitis C virus isolates by PCR J Clin Microbiol 32, 2031ā€“2034.

    PubMedĀ  CASĀ  Google ScholarĀ 

  39. Tuveri, R., Rotschild, C, Pol, S., Reijasse, D., Persico, T., Gazengel, C, et al (1997) Hepatitis C virus genotypes in French haemophiliacs: kinetics and reappraisal of mixed infections. J. Med. Virol. 51, 36ā€“41

    ArticleĀ  PubMedĀ  CASĀ  Google ScholarĀ 

  40. McOmish, F., Yap, P.-L., Dow, B., Follett, E., Seed, C., Keller, A, et al (1994) Geographical distribution of hepatitis C virus genotypes in blood donors. an international collaborative survey J. Clin. Microbiol 32, 884ā€“892.

    PubMedĀ  CASĀ  Google ScholarĀ 

  41. Simmonds, P., Rose, K., Graham, S., Chan, S.-W, McOmish, F., Dow, B., et al. (1993) Mapping of seotype-specific epitopes in the NS4 region of hepatitis C virus (HCV): use of type-specific peptides to serologically differentiate infections with HCV types 1, 2, and 3. J. Clin. Microbiol 31, 1493ā€“1503

    PubMedĀ  CASĀ  Google ScholarĀ 

  42. Battacherjee, V., Prescott, L, Pike, I, Rodgers, B, Bell, H., El-Zayadi, A, et al. (1995) Use of NS4 peptides to identify type-specific antibody to hepatitis C virus genotypes 1, 2, 3, 4, 5, and 6. J. Gen. Virol. 76, 1737ā€“1748.

    ArticleĀ  Google ScholarĀ 

  43. Martinot M, Marcellin, P, Pellet, C, Le Breton, V, Darthuy, F., RƩmirƩ, J, et al (1995) Serological assay to determine HCV genotype comparison with PCR based HCV genotyping Hepatology 22 (Pt. 4), 358A

    Google ScholarĀ 

  44. van Doorn, L.-J, Kleter, B, Pike, I, and Quint, W. (1996) Analysis of hepatitis C virus isolates by serotyping and genotyping. J Clin Microbiol 34, 1784ā€“1787.

    PubMedĀ  Google ScholarĀ 

  45. Saracco, G., Abate, M, Baldi, M, Calvo, P, Manzmi, P., Brunetto, M, et al (1994) Hepatitis C virus markers in patients with long-term biochemical and histological remission of chronic hepatitis Liver 14, 65ā€“70.

    PubMedĀ  CASĀ  Google ScholarĀ 

  46. Dixit, V, Quan, S., Martin, P., Larson, D, Brezina, M, DiNello, R, et al. (1995) Evaluation of a novel serotyping system for hepatitis C virus strong correlation with standard genotyping methodologies. J Clin Microbiol. 33, 2978ā€“2983.

    PubMedĀ  CASĀ  Google ScholarĀ 

  47. Lau, J., Davis, G, Prescott, L, Maertens, G, Lindsay, K., Qian, K, et al. (1996) Distribution of hepatitis C virus genotypes determined by a line probe assay in patients with chronic hepatitis C seen at trertiary referral centers in the united states. Ann Intern Med 124, 868ā€“876

    PubMedĀ  CASĀ  Google ScholarĀ 

  48. Huber, K, Knapp, M, and Bauer, K (1995) Subtyping of hepatitis C virus Clin Chem 41, 319,320

    PubMedĀ  CASĀ  Google ScholarĀ 

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Ā© 1998 Humana Press Inc., Totowa, NJ

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Maertens, G., Stuyver, L. (1998). HCV Genotyping by the Line Probe Assay INNO-LiPA HCV II. In: Lau, J.YN. (eds) Hepatitis C Protocols. Methods in Molecular Medicineā„¢, vol 19. Humana Press. https://doi.org/10.1385/0-89603-521-2:183

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  • DOI: https://doi.org/10.1385/0-89603-521-2:183

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-521-8

  • Online ISBN: 978-1-59259-260-9

  • eBook Packages: Springer Protocols

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