Abstract
Hepatitis C viruses (HCVs) constitute a highly variable genus within the Flaviviridae, with closest homology to the hepatitis G and GB viruses, and Pestiviruses. The positive-stranded RNA genome encodes a polyprotein which is co- and posttranslationally cleaved into at least nine proteins. Core, E1, and E2 (the structural proteins) and NS2, NS3, NS4A, NS4B, NS5A, and NS5B (the nonstructural [NS] proteins). A theoretical protein of 7 kDa (tp7) may be processed from the carboxy terminal E2 region (1).
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References
Maertens, G. and Stuyver, L. (1997) Genotypes and Genetic variation of hepatitis C virus, in Molecular Medicine of Viral Hepatitis (Zuckerman, A. and Harrison, T, eds.), Molecular Medical Science Series (James, K and Morris A, eds), John Wiley, Chichester, England, pp. 183ā233
Hoofnagle, J and Di Bisceglie, A. (1997) The treatment of chronic viral hepatitis N Engl J Med. 336, 347ā356
Chemello, L, Bonetti, P, Cavalletto, L, Talato, F., Donadon, V., Casarin, P, et al (1995) Randomized trial comparing three different regimens of alpha-2a-interferon in chronic hepatitis C Hepatology 22, 700ā706
Qu, D., Li, J-S, Vivitski, L., Mechai, S., Berby, F, Tong, S-P, et al (1994) Hepatitis C virus genotypes in France: comparison of clinical features of patients infected with HCV type I and type II. J Hepatol 21, 70ā75
Tsubota, A., Chayama, K, Ikeda, K., Yasuji, A., Koida, I., Saitoh, S, et al. (1994) Factors predictive of response to interferon-alfa therapy in hepatitis C infection. Hepatology 19, 1088ā1094.
Nousbaum, J-B, Pol, S, Nalpas, B., Landais, P., Berthelot, P., and BrĆ©chot, C (1995) Hepatitis C virus type lb (II) infection in France and Italy Ann Intern Med 111, 161ā168
El-Zayadi, A., Selim, O, El Haddad, S, and Hamdy, H. (1995) Combination treatment of alpha interferon-2b and Ribavirin in chronic hepatitis C genotype 4 patients resistant to interferon therapy [Abstract]. Hepatology 22 (Pt. 2), 152A
Maggi, F., Vatteroni, M, Fornai, C, Morrica, A, Giorgi, M, Bendinelli, M., et al. (1997) Subtype 2c hepatitis C virus is highly prevalent in Italy and is heterogeneous in the NS5A region. J Clin. Microbiol 35, 161ā164
Okamoto, H., Crovatto, M, Pozzato, G., FĆ©ray, C, BrĆ©chot, C, and Mishiro, S (1995) Molecular and clinical characteristics of the hepatitis C virus genotype 2c found in Italians in Italy and France Int Hepatol Commun 3, 161ā165
Chemello, L, Cavaletto, L., Bernardinello, E, Guido, M, Pontisso, P., and Alberti, A (1995) The effect of interferon alfa and ribavirin combination therapy in naive patients with chronic hepatitis C. J. Hepatol. 23 (Suppl. 2), 8ā12.
Gane, E., Naoumov, N., Qian, K.-P, Mondelli, M, Maertens, G, Portmann, B, et al. (1996) A longitudinal analysis of hepatitis C virus replication following liver transplantation. Gastroenterology 110, 167ā177.
Gane, E, Portmann, B, Naoumov, N., Smith, H., Underhill, J., Donaldson, P., et al. (1996) Long-term outcome of hepatitis C infection after liver transplantation. N Engl J Med. 334, 815ā820.
FĆ©ray, C, Gigou, M., Samuel, D., Paradis, V., Mishiro, S., Maertens, G, ReynĆØs, M, et al. (1995) Influence of the genotypes of hepatitis C virus on the severity of recurrent liver disease after liver transplantation. Gastroenterology 108, 1088ā1096
Di Martino, V, FĆ©ray, C, Saurini, F, Samuel, D, ReynĆØs, M, and Bismuth, H (1995) Relation between intra-hepatic replication, genotypes and recurrent liver disease due to hepatitis C virus after liver transplantation [Abstract]. J Hepatol. 23 (Suppl. 1), 152.
Chant, K., Kociuba, K., Munro, R., Crone, S., Kerrdige, R., Quin, J., et al (1994) Investigation of possible patient-to-patient transmission of hepatitis C in a hospital New South Wales Heath Bull 5, 47ā51.
Zignego, A., Ferri, C, Giannini, C, Monti, M., La Civita, L, Careccia, G., et al (1996) Hepatitis C virus genotype analysis in patients with type II mixed cryoglobulinemia Ann Intern Med 124, 31ā34.
Kanazawa, Y., Hayashi, N., Mita, E., Li, T., Hagiwara, H, Kasahara, A., et al (1994) Influence of viral quasispecies on effectiveness of interferon therapy in chronic hepatitis C patients. Hepatology 20, 1121ā1130.
Honda, M, Kaneko, S, Sakai, A., Unoura, M., Murakami, S., and Kobayashi, K (1994) Degree of hepatitis C virus quasispecies and progression to liver disease Hepatology 20, 1144ā1151.
Allander, T, Medin, C, Jacobson, S., Grillner, L., and Persson, M. (1994) Hepatitis C transmission in a hemodialysis unit: molecular evidence for spread of virus among patients not sharing equipment. J. Med Virol 43, 514ā419
Simmonds, P., Alberti, A., Alter, H., Bonino, F., Bradley, D.W., BrĆ©chot, C., et al (1994) A proposed system for the nomenclature of hepatitis C virus genotypes Hepatology 19, 1321ā1324.
Stuyver, L., van Arnhem, W, Wyseur, A., Hernandez, F, Delaporte, E, and Maertens, G (1994) Classification of hepatitis C viruses based on phylogenetics analysis of the E1 and NS5B regions and identification of 5 new subtypes. Proc Natl Acad Sci USA 91, 10,134ā10,138.
Tokita, H., Okamoto, H., Tsuda, F, Song, P, Nakata, S, Chosa, T., et al (1994) Hepatitis C virus variants from Vietnam are classifiable into the seventh, eighth, and ninth major genetic groups. Proc Natl Acad Sci USA 91, 11,022ā11,026
Stuyver, L., Wyseur, A, van Arnhem, W., Hernandez, F, and Maertens, G (1996) A second generation INNO-LiPA for hepatitis C virus genotyping. J. Clin Microbiol 34 (9), 2259ā2266.
Stuyver, L., Rossau, R., Wyseur, A., Duhamel, M., Vanderborght, B., Van Heuverswyn, H., et al. (1993) Typing of hepatitis C virus isolates and characterization of new subtypes using a line probe assay. J Gen. Virol 74, 1093ā1102.
Stuyver, L, Wyseur, A., Rombout, A, Louwagie, J, Scarcez, T, Verhofstede, C, et al (1997) Line probe assay for rapid detection of drug-selected mutations in the human immunodeficiency virus type 1 reverse transcriptase gene Antimicrob Agents Chemother. 41, 284ā291
Stuyver, L., Rossau, R., and Maertens, G. (1996) Line probe assays for the detection of HCV and HBV genotypes Antiviral Ther. 1 (Suppl. 3), 53ā57
Smith, D., Mellor, J, Jarvis, L, Davidson, F., Kolberg, J, Urdea, M., et al (1995) Variation of the hepatitis C virus 5ā² non-coding region. implications for secondary structure, virus detection and typing. J Gen. Virol 76, 1749ā1761.
van Doorn, L.-J., Kleter, B., Stuyver, L., Maertens, G., Brouwer, J., Schalm, S, et al. (1994) Analysis of hepatitis C virus genotypes by a line probe assay (LiPA) and correlation with antibody profiles J Hepatol 12, 122ā129
Mahaney, K., Tedeschi, V., Maertens, G., Di Bisceglie, A, Vergalla, J., Hoofnagle, J., et al. (1994) Genotypic analysis of hepatitis C virus in American patients. Hepatology 20, 1405ā1411.
Gianmni, C, Thiers, V, Nousbaum, J.-B, Stuyver, L., Maertens, G, and BrĆ©chot, C (1994) Comparative analysis of the hepatitis C virus core PCR and LiPA genotyping assays J Hepatol 23, 246ā253.
Zeuzem, S., Ruster, B., Lee, J.-H., Stripf, T, and Roth, K. (1995) Evaluation of a reverse hybridization assay for genotyping of hepatitis C virus J Hepatol 23, 654ā661.
Stuyver, L, Fretz, C, Esquivel, C, Boudifa, A, Jaulmes, D., Azar, N, et al (1996) HCV genotype analysis in apparantly healthy anti-HCV positive Parisian blood donors Transfusion 36, 552ā558.
Stuyver, L., Wyseur, A., van Arnhem, W, Lunel, F., Laurent-Puig, P., Pawlotsky J.-M., et al. (1995) Hepatitis C virus genotyping by means of 5ā²UR/Core line probe assays and molecular analysis of untypeable samples Virus Res 38, 137ā157
Mellor, J., Holmes, E., Jarvis, L., Yap, P.-L, and Simmonds, P (1995) Investigation of the pattern of hepatitis C virus sequence diversity in different geographical regionsā implications for virus classification J Gen Virol 76, 2493ā2507
Okamoto, H., Sugiyama, Y, Okada, S., Kurai, K, Akahane, Y., Sugai, Y, et al, (1992) Typing of hepatitis C virus by polymerase chain raction with type-specific primersā application to clinical surveys and tracing infectious sources J Gen. Virol 73, 673ā679
Cammarota, G, Maggi, F, Vatteroni, M., Da Prato, M., Barsanti, L, Bendinelli, M., et al. (1995) Partial nucleotide sequencing of six subtype 2c hepatitis C viruses detected in Italy J Clin Microbiol 33, 2781ā2784
Kleter, G, van Doorn, L.-J, Stuyver, L., Maertens, G., Brouwer, J, Schalm, S, et al. (1995) Rapid genotyping of hepatitis C virus RNA-isolates obtained from patients residing in Western Europe J. Med Virol. 47, 35ā42.
Andonov, A. and Chaudary, R. (1994) Genotyping of Canadian hepatitis C virus isolates by PCR J Clin Microbiol 32, 2031ā2034.
Tuveri, R., Rotschild, C, Pol, S., Reijasse, D., Persico, T., Gazengel, C, et al (1997) Hepatitis C virus genotypes in French haemophiliacs: kinetics and reappraisal of mixed infections. J. Med. Virol. 51, 36ā41
McOmish, F., Yap, P.-L., Dow, B., Follett, E., Seed, C., Keller, A, et al (1994) Geographical distribution of hepatitis C virus genotypes in blood donors. an international collaborative survey J. Clin. Microbiol 32, 884ā892.
Simmonds, P., Rose, K., Graham, S., Chan, S.-W, McOmish, F., Dow, B., et al. (1993) Mapping of seotype-specific epitopes in the NS4 region of hepatitis C virus (HCV): use of type-specific peptides to serologically differentiate infections with HCV types 1, 2, and 3. J. Clin. Microbiol 31, 1493ā1503
Battacherjee, V., Prescott, L, Pike, I, Rodgers, B, Bell, H., El-Zayadi, A, et al. (1995) Use of NS4 peptides to identify type-specific antibody to hepatitis C virus genotypes 1, 2, 3, 4, 5, and 6. J. Gen. Virol. 76, 1737ā1748.
Martinot M, Marcellin, P, Pellet, C, Le Breton, V, Darthuy, F., RƩmirƩ, J, et al (1995) Serological assay to determine HCV genotype comparison with PCR based HCV genotyping Hepatology 22 (Pt. 4), 358A
van Doorn, L.-J, Kleter, B, Pike, I, and Quint, W. (1996) Analysis of hepatitis C virus isolates by serotyping and genotyping. J Clin Microbiol 34, 1784ā1787.
Saracco, G., Abate, M, Baldi, M, Calvo, P, Manzmi, P., Brunetto, M, et al (1994) Hepatitis C virus markers in patients with long-term biochemical and histological remission of chronic hepatitis Liver 14, 65ā70.
Dixit, V, Quan, S., Martin, P., Larson, D, Brezina, M, DiNello, R, et al. (1995) Evaluation of a novel serotyping system for hepatitis C virus strong correlation with standard genotyping methodologies. J Clin Microbiol. 33, 2978ā2983.
Lau, J., Davis, G, Prescott, L, Maertens, G, Lindsay, K., Qian, K, et al. (1996) Distribution of hepatitis C virus genotypes determined by a line probe assay in patients with chronic hepatitis C seen at trertiary referral centers in the united states. Ann Intern Med 124, 868ā876
Huber, K, Knapp, M, and Bauer, K (1995) Subtyping of hepatitis C virus Clin Chem 41, 319,320
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Maertens, G., Stuyver, L. (1998). HCV Genotyping by the Line Probe Assay INNO-LiPA HCV II. In: Lau, J.YN. (eds) Hepatitis C Protocols. Methods in Molecular Medicineā¢, vol 19. Humana Press. https://doi.org/10.1385/0-89603-521-2:183
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DOI: https://doi.org/10.1385/0-89603-521-2:183
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