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Specific Detection of Negative Strand RNA of Hepatitis C Virus Using Chemical RNA Modification

  • Toshiaki Gunji
  • Kunitada Shimotohno
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 19)

Abstract

Hepatitis C virus (HCV), the genome of which was molecularly cloned in the US and Japan, causes most, if not all, cases of posttransfusional non-A, non-B hepatitis (NANBH) and nearly half those of sporadic hepatitis. Prospective and retrospective studies revealed that nearly half of patients with acute infection develop a chronic state of HCV infection, and half of them develop to liver cirrhosis and hepatocellular carcinoma.

Keywords

Negative Strand Sodium Acetate Solution Sodium Tetrahydroborate Sporadic Hepatitis Nonspecific Primer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Humana Press Inc., Totowa, NJ 1998

Authors and Affiliations

  • Toshiaki Gunji
    • 1
  • Kunitada Shimotohno
    • 2
  1. 1.Third Department of Internal MedicineTokyo UniversityTokyoJapan
  2. 2.Institute for Virus ResearchKyoto UniversityKyotoJapan

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