Specific Detection of Negative Strand RNA of Hepatitis C Virus Using Chemical RNA Modification

  • Toshiaki Gunji
  • Kunitada Shimotohno
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 19)


Hepatitis C virus (HCV), the genome of which was molecularly cloned in the US and Japan, causes most, if not all, cases of posttransfusional non-A, non-B hepatitis (NANBH) and nearly half those of sporadic hepatitis. Prospective and retrospective studies revealed that nearly half of patients with acute infection develop a chronic state of HCV infection, and half of them develop to liver cirrhosis and hepatocellular carcinoma.


Negative Strand Sodium Acetate Solution Sodium Tetrahydroborate Sporadic Hepatitis Nonspecific Primer 
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Copyright information

© Humana Press Inc., Totowa, NJ 1998

Authors and Affiliations

  • Toshiaki Gunji
    • 1
  • Kunitada Shimotohno
    • 2
  1. 1.Third Department of Internal MedicineTokyo UniversityTokyoJapan
  2. 2.Institute for Virus ResearchKyoto UniversityKyotoJapan

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