Detection of Hepatitis C Virus Quasispecies Heterogeneity by Single-Strand Conformational Polymorphism

  • Regino P. Gonález-Peralta
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 19)


Hepatitis C virus (HCV) is believed to replicate via a viral-encoded, RNA-dependent RNA polymerase. This replication strategy has limited fidelity Thus, HCV is genetically heterogenous. To date, six HCV genotypes and more than 80 viral subtypes have been identified. Further, even within individually infected patients, HCV exists as a highly heterogenous population of closely related genome called quasispecies (1, 2, 3). Viral quasispecies have been shown to have important pathobiological implications.


SSCP Analysis Viral Quasispecies Quasispecies Population Phast System Briefly Centrifuge 
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  1. 1.
    Martell, M., Esteban, J. I., Quer, J., Genesca, J., Weiner, A., Esteban, R., et al (1992) Hepatitis C virus circulates as a population of different but closely related genome, quasispecies nature of HCV genome distribution Virology 66, 3225–3229.Google Scholar
  2. 2.
    Higashi, Y, Kakumu, S, Yoshioka, K., Wakita, T, Mizokami, M, Ohba, K, et al (1993) Dynamics of genome change in the E2/NS1 region of hepatitis C virus in vivo. Virology 197, 659–668PubMedCrossRefGoogle Scholar
  3. 3.
    Weiner, A J., Brauer, M. J, Rosenblatt, J., Richman, K H., Tung, J, Crawford, K., et al (1991) Variable and hypervariable domains are found in the regions of HCV corresponding to the flavirus envelope and NS1 proteins and the pestivirus envelope glycoprotein Virology 180, 842–848PubMedCrossRefGoogle Scholar
  4. 4.
    González-Peralta, R. P., Qian, K., She, J Y., Davis, G. L, Ohno, T, Mizokami, M., et al. (1996) Clinical implications of viral quasispecies in chronic hepatitis C J. Med. Virol 49, 242–247.PubMedCrossRefGoogle Scholar
  5. 5.
    Davis, G L., Lau, J. Y N., Urdea, M. S, Neuwald, P., Wilber, J. C, Lindsay, K, et al. (1994) Quantitative detection of hepatitis C virus RNA by solid phase branched DNA amplification method definition and optimal conditions for specimen collection and clinical application in interferon-treated patients Hepatology 19, 1337–1341.PubMedCrossRefGoogle Scholar
  6. 6.
    Kwok, S and Higuchi, R (1989) Avoiding false positives with PCR. Nature 339, 237–238PubMedCrossRefGoogle Scholar
  7. 7.
    González-Peralta, R P, Liu, W Z, Davis, G. L, Qian, K, and Lau, J. Y N (1997) Modulation of hepatitis C virus quasispecies heterogeneity by interferon-and ribavirin therapy J Viral Hepatitis 4, 99–106Google Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 1998

Authors and Affiliations

  • Regino P. Gonález-Peralta

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