Molecular Evolutionary Analysis

Its Application in the Study of Hepatitis C Virus
  • Masashi Mizokami
  • Johnson Y. N. Lau
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 19)


The recent development of high-power personal computer hardware and software has allowed investigators to prepare phylogenetic trees without understanding of the fundamental principles of molecular evolution. Unfortunately, inexperienced investigators are also prone to draw inappropriate or wrong conclusions from this type of analysis. In particular, extreme caution should be taken when phylogenetic trees are applied to clinical settings.


Adenine Pyrimidine Purine Cytosine Guanine 


  1. 1.
    Nei, M. (1987) Molecular Evolutionary Genetics. Columbia University Press, New YorkGoogle Scholar
  2. 2.
    Kimura, M. (1993) The Neutral Theory of Molecular Evolution Cambridge University Press, CambridgeGoogle Scholar
  3. 3.
    Jukes, T. H. and Cantor, C R. (1969) Evolution of protein molecules, in Mammalian Protein Metabolism (Munro, H N., ed.), Academic, New York, pp 21–132Google Scholar
  4. 4.
    Kimura, M. (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences J Mol Evol 16, 111–120PubMedCrossRefGoogle Scholar
  5. 5.
    Gojobon, T., Ishii, K, and Nei, M. (1982) Estimation of average number of nucleotide substitutions when the rate of substitutions varies with nucleotide J Mol Evol 18, 414–423.CrossRefGoogle Scholar
  6. 6.
    Nei, M (1975) Molecular Population Genetics and Evolution North-Holland, Amsterdam.Google Scholar
  7. 7.
    Saitou, N and Nei, M (1987) The neighbor-joining method-a new method for reconstructing phylogenetic trees. Mol Biol Evol 3, 418–426Google Scholar
  8. 8.
    Felsenstein, J (1981) Evolutionary trees from DNA sequences, a maximum likelihood approach J Mol Evol 17, 368–376PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 1998

Authors and Affiliations

  • Masashi Mizokami
    • 1
  • Johnson Y. N. Lau
    • 2
  1. 1.Second Department of MedicineNagoya City University Medical SchoolNagoyaJapan
  2. 2.Research and DevelopmentICN PharmaceuticalsCosta Mesa

Personalised recommendations