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Bioluminescent Assay of the Guanylates

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Bioluminescence Methods and Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 102))

Abstract

The guanine nucleotides (rtbo and deoxyribo) serve many important roles in biosynthesis and btological control. They are involved in the synthesis of enzymes (1), and are allosteric regulators for such enzymes as glutamate dehydrogenase (2) and acetyl-CoA carboxylase (3). They regulate IMP dehydrogenase gene expression (4). The guanine nucleotldes are incorporated into DNA and RNA (5), are substrates in the Krebs tricarboxylic acid cycle (6), and function in microtubule assembly (7). Guanine nucleotides regulate the activation of adenylate cyclase vta G proteins in receptor-mediated signalmg processes (8–11). The G-proteins are involved in ion channel function (12). Guanylates also serve as coenzymes functioning in such physiological responses as vision (13), bactertal sporulation (14), and the stringent response to amino acid deprivation (15).

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK

    Sharon R. Ford & Franklin R. Leach

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  1. Sharon R. Ford
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  2. Franklin R. Leach
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Editors and Affiliations

  1. DuPont Co., Wilmington, DE

    Robert A. LaRossa

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© 1998 Humana Press Inc., Totowa, NJ

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Ford, S.R., Leach, F.R. (1998). Bioluminescent Assay of the Guanylates. In: LaRossa, R.A. (eds) Bioluminescence Methods and Protocols. Methods in Molecular Biology™, vol 102. Humana Press. https://doi.org/10.1385/0-89603-520-4:55

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  • DOI: https://doi.org/10.1385/0-89603-520-4:55

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-520-1

  • Online ISBN: 978-1-59259-577-8

  • eBook Packages: Springer Protocols

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