Skip to main content
SpringerLink
Log in

Bioluminescence-Based Metal Detectors

  • Protocol
Bioluminescence Methods and Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 102))

Abstract

This chapter consists of two methodologically rather divergent topics: the construction of a metal sensor bacterial strain and the measurement of bioavailable metal using such a strain. The constructton part IS written assummg that readers are famthar with basic recombinant DNA techniques, such as isolation and purificatron of DNA, the use of restriction enzymes, and ligation. However, if that is not the case, plasmids and bacterial strains for metal bioavailabtlity measurements are avarlable from the authors’ laboratory.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 79.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Tescione, L, and Belfort, G. (1993) Construction and evaluation of a metal ion biosensor. Blotechnol Bloeng 42, 945–952

    Article  CAS  Google Scholar 

  2. Kong, Z., van Rollegheim, P. A., and Verstraete, W. (1993) An activated sludgebased biosensor for rapid IC50 estimation and on-line toxicity monitormg. Blosens. Bloelectron 8, 49–58.

    Article  CAS  Google Scholar 

  3. Ribo, J. M. and Kaiser, K L. E. (1987) Photobactermm phosphoreum, toxicity bioassay. Toxtcol. Assess. 2, 305–323.

    Article  CAS  Google Scholar 

  4. Lampinen, J., Korpela, M., Saviranta, P., Kroneld, R., and Karp, M. (1990) Use of Escherichza Coli Cloned with genes Encoding Bacterial Luciferase for Evaluation of Chemical Toxicity. Toxiol Assess. 5, 337–350.

    Article  CAS  Google Scholar 

  5. Lampinen, J., Vtrta, M., and Karp, M. (1995) Use of controlled luciferase expression for monitormg of chemicals affecting protein synthesis. Appl Envwon. Microbial. 61, 2981–2989

    CAS  Google Scholar 

  6. Holland, G. J., and Green, A. (1975) Development of a gross pollution detector: laboratory studies. Water Treatment Exam 4, 8l–99

    Google Scholar 

  7. Virta, M., Lampmen, J., and Karp, M. (1995) A lummescence-based mercury biosensor. Anal Chem. 67, 667–669

    Article  CAS  Google Scholar 

  8. King, J. M. H., DiGrazia, P. M, Applegate, B., Burlage, R., Sansevermo, J., Dunbar, P., Larimer, F., and Sayer, G. S. (1990) Rapid, sensitive bioluminescent reporter technology for naphtalene exposure and biodegradation. Science 249, 778–781

    Article  PubMed  CAS  Google Scholar 

  9. deWet, J. R, Wood, K. V., DeLuca, M., Helinski, D. R., and Subramani, S. (1987) The firefly luciferase gene: structure and expression in mammalian cells. Mol Cell Blol. 7, 725–737

    Google Scholar 

  10. Li, Z and Meighen, E. A. (1994) The turnover of bacterial luciferase is limited by a slow decomposition of the ternary enzyme-product complex of luciferase, FMN, and fatty acid. J BEOZ Chem 269, 6640–6644

    CAS  Google Scholar 

  11. Summers, A O. (1986) Organization, expression, and evolution of genes for mercury resistance. Ann. Rev Mrcrobiol. 40, 607–634.

    Article  CAS  Google Scholar 

  12. Silver, S. K., Budd, K. M., Leahy, W. V., Shawn, D., Hammond, R. P., Novick, G.R., Wilsky, M. H., Malamy, H M., and Rosenberg, H. (1981) Inducible plasmid-determined resistance to arsenate, arsenite and antimony (III) in Escherichza coli and Staphylococcus aureus. J Bacterial 146, 983–996.

    CAS  Google Scholar 

  13. Nucifora, G., Chu, L, Misra, T. K., and Silver, S. (1989) Cadmium resistance from Staphylococcus aureus plasmid PI258 cadA gene results from cadmiumefflux ATPase. Proc. Nat1 Acad Set. USA 86, 3544–3548.

    Article  CAS  Google Scholar 

  14. Nies, D H. and Silver, S (1989) Plasmid-determined mdicible efflux is responsible for resistance to cadmium, zinc, and cobalt in Alcaligenes eutrophus J. Bacterial. 171, 896–900.

    CAS  Google Scholar 

  15. Cha, J S. and Cooksey, D. A (1991) Copper resistance in Pseudomonas syrzngae mediated by periplasmic and outer membrane proteins. Proc Natl. Acad Set USA 88, 8915–8919.

    Article  CAS  Google Scholar 

  16. Farrell, R. E., Germida, J J., and Huang, P M. (1993) Effects ofchemical speciatton in growth media on the toxicity of mercury (II). Appl Environ Mzcrobtol. 59, 1507–1514

    CAS  Google Scholar 

  17. Lampinen, J., Koivisto, L, Wahlsten, M., Mantsala, P., and Karp, M (1992) Expression of luciferase genes from different origins in Bacillus subttlu. Mol Gen. Genet. 232, 498–504

    Article  PubMed  CAS  Google Scholar 

  18. Sambrook, J, Fritsch, E F., and Mamatis, T (1989) Molecular Cloning: A Laboratory Manual Cold Spring Harbor Laboratory, Cold Sprmg Harbor, New York

    Google Scholar 

  19. Ji, G and Silver, S (1992) Regulation and expression of the arsenic resistance operon from Staphylococcus aureus plasmid p1258 J Bactertol. 174, 3684–3694.

    CAS  Google Scholar 

  20. Wood, K. V. and DeLuca, M (1987) Photographic detection of luminescence in Escherzchta colt containing the gene for firefly luciferase. Anal Btochem 161, 50 l–507

    Google Scholar 

  21. Dower, W J., Miller, J. F., and Ragsdale, C. W. (1988) High efficiency transformation of E. coli by high voltage electroporation. Nucleic Acid Res 16, 6126–6144.

    Article  Google Scholar 

  22. Casabadan, M. J. and Cohen, S. N. (1978) Analysis of gene control signals by DNA fusion and cloning in Escherrchia coli. J Mol. Btol. 138, 179–207.

    Article  Google Scholar 

  23. Schenk, S, and Laddaga, R. A (1992) Improved method for electroporation of Staphylococcus aureus FEMS Microbtol. Lett. 94, 133–138.

    Article  CAS  Google Scholar 

  24. Kreiswuth, B. N., Lofdahl, M. J., O’Reilly, M., Schlievert, P. M., Bergdoll, M S, and Novick, R. P. (1993) The toxic shock syndrome exotoxm structural gene is not detectably transmitted by a prophage. Nature 305, 709

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biotechnology, University of Turku, Finland

    Marko Virta & Matti Karp

  2. Department of Biochemistry and Pharmacy, Abo Akademi University, Turku, Finland

    Sisko Tauriainen

Authors
  1. Marko Virta
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sisko Tauriainen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Matti Karp
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. DuPont Co., Wilmington, DE

    Robert A. LaRossa

Rights and permissions

Reprints and permissions

Copyright information

© 1998 Humana Press Inc., Totowa, NJ

About this protocol

Cite this protocol

Virta, M., Tauriainen, S., Karp, M. (1998). Bioluminescence-Based Metal Detectors. In: LaRossa, R.A. (eds) Bioluminescence Methods and Protocols. Methods in Molecular Biology™, vol 102. Humana Press. https://doi.org/10.1385/0-89603-520-4:219

Download citation

  • DOI: https://doi.org/10.1385/0-89603-520-4:219

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-520-1

  • Online ISBN: 978-1-59259-577-8

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation