Abstract
Phagocytes form an essential defence against microbial infection and have an important role in debridement following tissue injury. In human subjects, there are essentially two classes of phagocyte: polymorphnuclear leukocytes (PMNL) and mononuclear phagocytes, both derived from myelomonocytic bone marrow cells. PMNL circulate in the bloodstream, and are subdivided into neutrophils, eosinophils, and basophiles. Mononuclear phagocytes circulate in the blood as monocytes as a heterogeneous population, a proportion of which becomes tissue macrophages (e.g., Kupffer cells in liver, microgha in brain). While blood-borne PMNL and monocytes are easily accessible for study, many tissue macrophages are difficult to obtain for in vitro chemiluminescence. Although methods of obtaining relatively pure populations of tissue macrophages exist, all tend to activate the cells to a variable degree, which impedes interpretation of any results obtained. Most in vitro macrophage studies are therefore conducted with monocyte-derived cells, although alveolar (lung) and peritoneal macrophages can be studied by direct sampling with minima1 preparation.
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References
Segal A. W. (1993) Structure of the NADPH-oxidase: membrane components Immunodeficiency 4, 167–179.
Allen R. C., Stemholm R. L., and Steele R. H. (1972) Evtdence for the generation of an electronic excitation state(s) in human polymorphonuclear leukocytes and its participatton in bacterial activity. Biochem. Bzophys Res Commun 47, 679–684.
Blair A. L, Cree I. A., Beck J. S., and Hastings M. J. G (1988) Measurement of phagocyte chemiluminescence in a microtitre plate format. J Immunol. Methods 112, 163–168.
Lundqvist H., Kricka L. J., Stott R. A., Thorpe G. H., and Dahlgren C. (1995) Influence of different lummols on the characteristics of the chemiluminescence reaction in human neutrophils. J Biolumzn. Chemllumm 10, 353–359.
McCafferty A C., Cree I. A., and McMurdo M. E. T. (1995) The influence of age and sex on phagocyte chemiluminescence. J Biolummescence Chemduminescence 10, 41–48
Ramage L., Blair A. L., Cree I. A., and Dhillon D. P. (1993) Effect of salmeterol on polymorphonuclear leukocyte (PMNL) function in vitro J Blolummescence Chemiluminescence 8, 247–252.
Cree I. A. (1991) Assays of human phagocyte function using microtitre plate luminometers, in Bioluminescence and Chemrluminescence. Current Status. (Stanley P. E and Kricka L. J, eds.), John, Chichester, pp 261–264.
Ramage L., Cree I. A., and Dhillon D. P. (1994) Comparison of salmeterol with placebo in mild asthma: effect on peripheral blood phagocyte function and cytokine levels. Znt Arch Allergy Immunol. 105, 18l–l84.
Cree I A (1993) Longitudinal studies of phagocyte chemilummescence in pattents with lung disease, in Chemiiuminescence and Blolumvzescence. Status Report (Szalay A, Kricka L. J, and Stanley P. E., eds), John, Chichester, 45l–455.
Moussa K., Michie H J., Cree I. A., MacCafferty A., Winter J H., and Brown R. A. (1994) Phagocyte function and cytokine production in community-acquired pneumonia. Thorax 49, 107–111
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© 1998 Humana Press Inc., Totowa, NJ
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Cree, I.A. (1998). Phagocyte Chemiluminescence. In: LaRossa, R.A. (eds) Bioluminescence Methods and Protocols. Methods in Molecular Biology™, vol 102. Humana Press. https://doi.org/10.1385/0-89603-520-4:179
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DOI: https://doi.org/10.1385/0-89603-520-4:179
Publisher Name: Humana Press
Print ISBN: 978-0-89603-520-1
Online ISBN: 978-1-59259-577-8
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