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Luminometric Measurement of Malate and Glucose-6-Phosphate in Mammalian Tissue

  • Eberhard Jüngling
  • Helmut Kammermeier
  • Yvan Fischer
Part of the Methods in Molecular Biology™ book series (MIMB, volume 102)

Abstract

When using cells of mammmalian origin, the amount of biological material available for analytical purposes is often limited (e.g., ~l05 cells/sample, cor responding to few mg tissue). Since most intermediary metabolites are found in a concentratron range of ~50-500 μmol/g in a wide variety of tissues (see, e.g., ref. 1), only nanomolar or subnanomolar quantities can be expected to be present in small samples obtained from isolated cells or cell cultures. Such quantities are at best barely detectable by conventional spectrophotometric or fluorimetric procedures

Keywords

Ammonium Sulfate Biological Matrix Flavin Mononucleotide Intermediary Metabolite Start Reaction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 1998

Authors and Affiliations

  • Eberhard Jüngling
    • 1
  • Helmut Kammermeier
    • 1
  • Yvan Fischer
    • 1
  1. 1.Institute of PhysiologyMedical FacultyGermany

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