Real-Time Reporter of Protein Synthesis Inhibition

  • Matti Korpela
  • Marko Virta
  • Matti Karp
Part of the Methods in Molecular Biology™ book series (MIMB, volume 102)


In studies concerning drug—receptor interaction, the fundamental need is the knowledge of theevents that take place when a drug and a receptor combine. The current knowledge of microbial cell structure at the molecular level is restricted so that very often unsatisfymgly indirect methods must be used. The methods of analysis may be too severely narrowed down. There is a big risk in drawing conclusions without any real evidence of immediate responses to drugs by living bacteria.


Light Production Bacterial Luciferase Microplate Luminometer Aldehyde Substrate Tube Luminometer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Cohn, D. H., Mileham, A. J., Simon, M. I., Nealson, K. H., Rausch, S. K, Bonam, D., and Baldwin, T.O. (1985) Nucleotide sequence of the luxA gene of Vibrioharveyi and the complete amino acid sequence of the a subunit of bacterial luci-ferase J Biol Chem. 260, 6139–6146.PubMedGoogle Scholar
  2. 2.
    Johnston, T. C, Thompson, R, B., and Baldwin, T. O (1986) Nucleotide sequence of the 1uxB gene of Vibrio harveyi and the complete ammo acid sequence of the \ subunit of bacterial luciferase. J Biol. Chem. 261, 4805–4811.PubMedGoogle Scholar
  3. 3.
    Hastings,_J. W., Baldwin, T.O, and Nicoli, M. Z. (1978) Bacterial luciferase. Assay, purification and properties, in Methods in Enzymology, vol. 57 (DeLuca, M., ed.),pp. 135–152.Google Scholar
  4. 4.
    Bernard, H. U., Remaut, M. V., Hersfield, H. K., Das, D. R., Helinski, C., Yanofsky, C., and Franklin, N. (1979) Construction of plasmid cloning vehicles that promote gene expression from the bacteriophage λ pL promoter. Gene 5, 59–76.PubMedCrossRefGoogle Scholar
  5. 5.
    Stanssens, P., Remaut, E, and Fiers, W. (1985) Alterations upstream from the Shme-Dalgarno region and then effect on bacterial gene expression Gene 39, 441–453Google Scholar
  6. 6.
    Gupta, S. C., Reese, C P., and Hastings, J. W. (1986) Mobilization of cloned luci-ferase genes into Vibrzo harveyi luminescence mutants. Arch. Mzcrobzol. 143, 325–329.CrossRefGoogle Scholar
  7. 7.
    Meighen, E. A., Riendeau, D, and Bognar, A. (1981) Bacterial bioluminescence. accessory enzymes, in Bioluminescence and Chemiluminescence Basic Chemis-try and Analytical Applications (DeLuca, M. and McElroy, W. D., eds.), Aca-demic, New York, pp. 129–138Google Scholar
  8. 8.
    McElroy, W D. and DeLuca, M. (1985) Firefly lummescence, In Chemi-and bio-Zuminescence (J. G. Burr, J G, ed.), Marcel Dekker, New York., pp. 387–399.Google Scholar
  9. 9.
    Lampinen, J., Korpela, M., Saviranta, P., Kroneld, R., and Karp, M (1990) Use of Escherichia coli cloned with genes encoding bacterial luciferase for evaluation of chemical toxicity Toxic Assess 5, 337–350.CrossRefGoogle Scholar
  10. 10.
    Lampinen, J., Virta, M., and Karp, M. (1995) Comparison of Gram positive and Gram negative bacterial strains cloned with different types of luciferase genes in bioluminescence cytotoxicity tests. Environ. Toxicol. Water Qual. 10, 157–166.CrossRefGoogle Scholar
  11. 11.
    Virta, M., Karp, M., and Vuorinen, P. (1994) Nitric oxide donor-mediated killing of bioluminescent Escherichia coli. Antimicrob. Agents Chemother. 38, 2775–2779.Google Scholar
  12. 12.
    Koncz, C., Langridge, W. H., Olsson, O., Schell, J, and Szalay, A. A. (1990) Bacterial and firefly luciferase genes in transgeinc plants. advantages and disad-vantages of a reporter gene. Dev Genet 11, 224–232.CrossRefGoogle Scholar
  13. 13.
    Lampinen, J., Virta, M., and Karp, M. (1995) Use of controlled luciferase expres-sion to monitor chemicals affecting protein synthesis Appl Environ Microbiol 61(8), 298l–2989.Google Scholar
  14. 14.
    Lövgren, T., Peacock, R., Lavi, J., Karp, M., and Raunio, R. (1982) The biolumi-nescent assay of NADH and NADPH. Int Lab 12, 58–61.Google Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 1998

Authors and Affiliations

  • Matti Korpela
    • 1
  • Marko Virta
    • 1
  • Matti Karp
    • 1
  1. 1.Department of BiotechnologyUniversity of TurkuFinland

Personalised recommendations