Targeting Antipeptide Antibodies Towards Cytochrome P450 Enzymes

  • Robert J. Edwards
Part of the Methods in Molecular Biology™ book series (MIMB, volume 107)

Abstract

Antibodies against cytochrome P450 enzymes can be produced by immunizing animals with synthetic peptides that mimic small regions of the apoprotein. As the amino-acid sequences of a large number of cytochrome P450 enzymes are known (1), this approach to raising antibodies can be applied to most of the major cytochrome P450 enzymes in human, rat and other species. This is a relattvely simple, rapid and effective method of producmg antibodies.

Keywords

Cysteine Thiol Diethyl Glutaraldehyde Lysozyme 

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Copyright information

© Humana Press Inc. 1998

Authors and Affiliations

  • Robert J. Edwards
    • 1
  1. 1.Section on Clinical PharmacologyImperial College School of MedicineLondonUK

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