A Stochastic PCR Approach for RNA Quantification in Multiple Samples

  • Adrian Puntschart
  • Michael Vogt
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 26)

Abstract

When studying the effect of various treatments on gene expression in humans, one occasionally is faced with the problem of detecting small changes in transcript levels in minute tissue samples. In addition, interindividual variations can be quite large and may even be the major source of variation (1). Therefore, numerous samples usually have to be analyzed to detect such small variations in gene expression.

Keywords

Vortex Phenol EDTA Bromide Electrophoresis 

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Copyright information

© Humana Press Inc., Totowa, NJ 1999

Authors and Affiliations

  • Adrian Puntschart
    • 1
  • Michael Vogt
    • 1
  1. 1.Department of AnatomyUniversity of BernSwitzerland

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