Comparison of Competitive PCR and Positive Control-Based PCR

  • François Mallet
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 26)

Abstract

The exponential amplification of small amounts of nucleic acids makes polymerase chain reaction (PCR) not only powerful but also challenging as a quantitative method. Variations in nucleic acid preparation, thermal cyclic performance, the choice of the polymerase, and the amplification procedure can cause large differences in final product yield. To address the challenges of quantitative PCR, the procedure has been critically examined, leading to an understanding of the critical parameters involved in quantitative amplification. Accepted parameters can be summarized as a series of choices: external vs internal standard, exogenous vs endogenous standard, competitive vs noncompetitive amplification, and exponential vs plateau amplification (1, 2, 3).

Keywords

Bromide Titration Tuberculosis MgCl2 Ethidium 

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Copyright information

© Humana Press Inc., Totowa, NJ 1999

Authors and Affiliations

  • François Mallet
    • 1
  1. 1.Unité Mixte de Recherche, CNRS-BioMérieuxEcole Normale Supérieure de LyonFrance

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