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Quantitative Measurement of the Biological Response of Cartilage to Mechanical Deformation

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Tissue Engineering Methods and Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 18))

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Abstract

Cartilage functionality is defined, in part, in terms of the ability of the extracellular matrix to support a mechanical load. It has been shown that such mechanical loading can influence the biological response of the chondrocytes that are embedded in the extracellular matrix. Cultured tissue explants have served as useful models for studying such chondrocyte-mediated responses to mechanical deformation. The explant paradigm facilitates the control of mechanical and biological variables that may influence cellular behavior. This chapter presents the means for assessing the biologic response of cartilage to controlled mechanical stimuli, and lays the foundation to further explore the response of cartilage to mechanical stimuli in the presence of other factors, such as cytokines and growth factors (e.g., IL-1β, TGF-β, IGF).

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Author information

Authors and Affiliations

  1. Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA

    R. Gregory Allen & Martha L. Gray

  2. Department of Biomedical Engineering, Boston University, Boston, MA

    Solomon R. Eisenberg

Authors
  1. R. Gregory Allen
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  2. Solomon R. Eisenberg
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  3. Martha L. Gray
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Editor information

Editors and Affiliations

  1. Center for Engineering in Medicine, Massachusetts General Hospital/Harvard Medical School, Boston, MA

    Jeffrey R. Morgan PhD  & Martin L. Yarmush MD  & 

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© 1999 Humana Press Inc., Totowa, NJ

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Allen, R.G., Eisenberg, S.R., Gray, M.L. (1999). Quantitative Measurement of the Biological Response of Cartilage to Mechanical Deformation. In: Morgan, J.R., Yarmush, M.L. (eds) Tissue Engineering Methods and Protocols. Methods in Molecular Medicine™, vol 18. Humana Press. https://doi.org/10.1385/0-89603-516-6:521

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  • DOI: https://doi.org/10.1385/0-89603-516-6:521

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-516-4

  • Online ISBN: 978-1-59259-602-7

  • eBook Packages: Springer Protocols

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