Abstract
Cell-cell and cell-matrix contacts play an important role in immunological processes and tissue organization. It has been shown that in many cases, cell adhesion is mediated by specialized adhesion receptors that are typically anchored in the cell membrane with mol wt ranging from 50 to 200 kDa. Binding of adhesion receptors anchored in the apposing cell membranes with their counterligands appears to be critical for many forms of cell-cell communication (1).
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References
Springer, T. A. (1995) Traffic signals on endothelium for lymphocyte recirculation and leukocyte emigration. Annu. Rev. Physiol. 57, 827ā872.
Xiao, Y. and Truskey, G. A. (1996) Effect of receptor-ligand affinity on the strength of endothelial cell adhesion. Biophys. J. 71, 2869ā2884.
Abbassi, O., Kishimoto, T. K., McIntire, L. V., Anderson, D. C, and Smith, C. W. (1993) E-selectin supports neutrophil rolling in vitro under conditions of flow. J. Clin. Invest. 92, 2719ā2730.
Buttrum, S. M., Hatton, R., and Nash, G. B. (1993) Selectin-mediated rolling of neutrophils on immobilized platelets. Blood 82, 1165ā1174.
Kaplanski, G., Farnarier, C., Tissot, O., Pierres, A., Benoliel, A. M., Alessi, M. C., Kaplanski, S., and Bongrand, P. (1993) Granulocyte-endothelium initial adhesion. Analysis of transient binding events mediated by E-selectin in a laminar shear flow. Biophys. J. 64, 1922ā1933.
Alon, R., Hammer, D. A., and Springer, T. A. (1995) Lifetime of the P-selectin-carbohydrate bond and its response to tensile force in hydrodynamic flow. Nature (Lond.) 374, 539ā542.
Lawrence, M. B., Kansas, G. S., Kunkel, E. J., and Ley, K. (1997) Threshold levels of fluid shear promote leukocyte adhesion through selectins (CD62L,P,E). J. Cell Biol. 136, 717ā727.
Capo, C., Garrouste, E., Benoliel, A., Bongrand, P., Ryter, A., and Bell, G. I. (1982) Concanavalin-A-mediated thymocyte agglutination: a model for a quantitative study of cell adhesion. J. Cell Sci. 56, 21ā48.
Taylor, A. D., Neelamegham, S., Heliums, J. D., Smith, C. W., and Simon, S. I. (1996) Molecular dynamics of the transition from L-selectin to Ī²2 integrin-dependent neutrophil adhesion under defined hydrodynamic shear. Biophys. J. 71, 3488ā3500.
Goldman, A. J., Cox, R. G., and Brenner, H. (1967) Slow viscous motion of a sphere parallel to a plane wall. II. Couette flow. Chem. Eng. Sci. 22, 653ā660.
Folie, B. J. and McIntire, L. V. (1989) Mathematical analysis of mural thrombogenesis. Concentration profiles of platelet-activating agents and effects of viscous shear flow. Biophys. J. 56, 1121ā1141.
Munn, L. L., Melder, R. J., and Jain, R. K. (1994) Analysis of cell flux in the parallel plate flow chamber: implications for cell capture studies. Biophys. J. 67, 889ā895.
Stamper, H. B., Jr. and Woodruff, J. J. (1976) Lymphocyte homing into lymph nodes in vitro demonstration of the selective affinity of recirculating lymphocytes for high-endothelial venules. J. Exp. Med. 144, 828ā833.
Dustin, M. L. and Springer, T. A. (1989) T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1. Nature (Lond.) 341, 41ā46.
Smith, C. W., Rothlein, R., Hughes, B. J., Mariscalco, M. M., Schmalstieg, F. C., and Anderson, D. C. (1988) Recognition of an endothelial determinant for CD18-dependent neutrophil adherence and transendothelial migration. J. Clin. Invest. 82, 1746ā1756.
Smith, C. W., Kishimoto, T. K., Abbassi, O., Hughes, B., Rothlein, R., McIntire, L. V., Butcher, E. C., and Anderson, D. C. (1991) Chemotactic factors regulate lectin adhesion molecule 1 (LECAM-1 )-dependent neutrophil adhesion to cytokine-stimulated endothelial cells in vitro. J. Clin. Invest. 87, 609ā618.
McClay, D. R., Wessel, G. M., and Marchase, R. B. (1981) Intercellular recognition: quantitation of initial binding events. Proc. Natl. Acad. Sci. USA 78, 4975ā4979.
Ward, M. D., Dembo, M., and Hammer, D. A. (1995) Kinetics of cell detachment: effect of ligand density. Ann. Biomed. Eng. 23, 322ā331.
Xia, Z. and Frojmovic, M. M. (1994) Aggregation efficiency of activated normal or fixed platelets in a simple shear field: effect of shear and fibrinogen occupancy. Biophys. J. 66, 2190ā2201.
Bell, D. N., Spain, S., and Goldsmith, H. L. (1989) Adenosine disphosphate-induced aggregation of human platelets in flow through tubes. I. Measurement of concentration and size of single platelets and aggregates. Biophys. J. 56, 817ā828.
Bargatze, R. F., Kurk, S., Butcher, E. C., and Jutila, M. A. (1994) Neutrophils roll on adherent neutrophils bound to cytokine-induced endothelial cells via L-selectin on the rolling cells. J. Exp. Med. 180, 1785ā1792.
Kuo, S. C. and Lauffenburger, D. A. (1993) Relationship between receptor/ligand binding affinity and adhesion strength. Biophys. J. 65, 2191ā2200.
Sung, L. A., Kabat, E. A., and Chien, S. (1985) Interaction energies in lectin-mediated erythrocyte aggregation. J. Cell Biol. 101, 652ā659.
Palecek, S. R., Loftus, J. C., Ginsberg, M. H., Lauffenburger, D. A., and Horwitz, A. F. (1997) Integrin-ligand binding properties govern cell migration speed through cell-substratum adhesiveness. Nature (Lond.) 385, 537ā540.
Truskey, G. A. and Pirone, J. S. (1990) The effect of fluid shear stress upon cell adhesion to fibronectin-treated surfaces. J. Biomed. Mater. Res. 24, 1333ā1353.
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Ramos, C.L., Lawrence, M.B. (1999). Quantitative Measurement of Cell-Cell Adhesion Under Flow Conditions. In: Morgan, J.R., Yarmush, M.L. (eds) Tissue Engineering Methods and Protocols. Methods in Molecular Medicineā¢, vol 18. Humana Press. https://doi.org/10.1385/0-89603-516-6:507
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DOI: https://doi.org/10.1385/0-89603-516-6:507
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