Abstract
Re-epithelialization is defined as the reconstitution of cells into an organized, stratified squamous epithelium that permanently covers a wound defect and restores function (1). Following wounding, keratinocytes are activated to undergo a series of phenotypic changes that have been well-characterized in vivo (2–4). However, in vitro studies of re-epithelialization have often been limited by their inability to simulate the in vivo tissue. Wound models using skin explants (5–8) or submerged keratinocyte cultures (9,10) demonstrate only partial differentiation and hyperproliferative growth. These systems have been useful for studying keratinoctye migration (11), but are limited in studying other aspects of re-epithelialization.
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© 1999 Humana Press Inc., Totowa, NJ
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Vaccariello, M.A., Javaherian, A., Parenteau, N., Garlick, J.A. (1999). Use of Skin Equivalent Technology in a Wound Healing Model. In: Morgan, J.R., Yarmush, M.L. (eds) Tissue Engineering Methods and Protocols. Methods in Molecular Medicine™, vol 18. Humana Press. https://doi.org/10.1385/0-89603-516-6:391
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DOI: https://doi.org/10.1385/0-89603-516-6:391
Publisher Name: Humana Press
Print ISBN: 978-0-89603-516-4
Online ISBN: 978-1-59259-602-7
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