Abstract
Microencapsulation of biologically active material in the form of artificial cell was reported as early as 1964 (1–4). However, it is only in the past 10 yr that many centers have extensively developed this (5). More recently, we have concentrated on three areas of artificial cells for blood substitutes, enzyme therapy, and cell therapy. Space allows only a few examples to be given here.
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References
Chang, T. M. S. (1964) Semipermeable microcapsules. Science 146, 524,525.
Chang, T. M. S., MacIntosh, F. C, and Mason, S. G. (1966) Semipermeable aqueous microcapsules: I. Preparation and properties. Can. J. Physiol. Pharmacol. 44, 115–128.
Chang, T. M. S., MacIntosh, F. C, and Mason, S. G. (1971) Encapsulated hydrophilic compositions and methods of making them. Canadian Patent 873,815,1971.
Chang, T. M. S. (1972) Artificial Cells, Monograph, Charles C Thomas, Springfield, IL.
Chang, T. M. S. (1995) Artificial cells with emphasis on bioencapsulation in biotechnology. Biotechnol. Annu. Rev. 2, 267–295.
Chang, T. M. S. (1997) Recent and future developments in modified hemoglobin and microencapsulated hemoglobin as red blood cell substiutes. Artif. Cells Blood Substitutes Immobilization Biotechnol. 25, 1–24.
Chang, T. M. S. (1997) Blood Substitutes: Principles, Methods, Products and Clinical Trials. Karger/Landes, Austin, TX.
Chang, T. M. S. and Yu, W. P. (1996) Biodegradable polymer membrane containing hemoglobin for blood substitutes. U. S. A. Patent approved in 1996.
Yu, W. P. and Chang, T. M. S. (1996) Submicron polymer membrane hemoglobin nanocapsules as potential blood substitutes: preparation and characterization. Artif. Cells Blood Substitutes Immobilization Biotechnol. 24, 169–184.
Chang, T. M. S. and Poznansky, M. J. (1968) Semipermeable microcapsules containing catalase for enzyme replacement in acatalsaemic mice. Nature 218, 242–245.
Chang, T. M. S. (1971) The in vivo effects of semipermeable microcapsules containing L-asparaginase on 6°C3HED lymphosarcoma. Nature 229, 117,118.
Chang, T. M. S. (1989) Preparation and characterization of xanthine oxidase immobilized by microencapsulation in artificial cells for the removal of hypoxanthine. J. Biomater. Artif Cells Artif. Organs 17, 611–616.
Palmour, R. M., Goodyer, P., Reade, T., and Chang, T. M. S. (1989) Microencapsulated xanthine oxidase as experimental therapy in Lesch-Nyhan Disease. Lancet 2, 687,688.
Chang, T. M. S., Bourget, L., and Lister, C. (1992) Orgal administration of microcapsules for removal of amino acids, US Patent No 5,147,641.
Chang, T. M. S., Bourget, L., and Lister, C. (1995) A new theory of enterorecirculation of amino acids and its use for depleting unwanted amino acids using oral enzymeartificial cells, as in removing phenylalanine in phenylketonuria. Artif. Cells Blood Substitutes Immobilization Biotechnol. 25, 1–23.
Bourget, L. and Chang, T. M. S. (1986) Phenylalanine ammonia-lyase immobilized in microcapsules for the depletion of phenylalanine in plasma in phenylketonuric rat model. Biochim. Biophys. Acta 883, 432–438.
Safos, S. and Chang, T. M. S. (1995) Enzyme replacement therapy in ENU2 phenylketonuric mice using oral microencapsulated phenylalanine ammonia-lyase: a preliminary report. Artif. Cells Blood Substitutes Immobilization Biotechnol. 25, 681–692.
Chang, T. M. S. (1985) Artificial cells with regenerating multienzyme systems. Methods Enzymol. 112, 195–203.
Gu, K. F., Chang, T. M. S. (1990) Production of essential L-branched-chained amino acids, in bioreactors containing artificial cells immobilized multienzyme systems and dextran-NAD+. Appl. Biochem. Biotechnol. 26, 263–269.
Chang, T. M. S. (1965) Semipermeable aqueous microcapsules. PhD Thesis. McGill University.
Lim, F. and Sun, A. M. (1980) Microencapsulated islets as bioartificial endocrine pancreas. Science 210, 908–909
Wong, H. and Chang, T. M. S. (1986) Bioartificial liver: implanted artificial cells microencapsulated living hepatocytes increases survival of liver failure rats. Int. J. Artif. Organs 9, 335,336.
Wong, H. and Chang, T. M. S. (1988) The viability and regeneration of artificial cell microencapsulated rat hepatocyte xenograft transplants in mice. J. Biomater. Artif. Cells Artif. Organs 16, 731–740.
Kashani, S. and Chang, T. M. S. (1991) Effects of hepatic stimulatory factor released from free or microencapsulated hepatocytes on galactosamine induced fulminant hepatic failure animal model. J. Biomater. Artif. Cells Immobilization Biotechnol. 19, 579–598.
Bruni, S. and Chang, T. M. S. (1989) Hepatocytes immobilized by microencapsulation in artificial cells: effects on hyperbilirubinemia in Gunn Rats. J. Biomater. Artif. Cells Artif. Organs 17, 403–12.
Bruni, S. and Chang, T. M. S. (1991) Encapsulated hepatocytes for controlling hyper-bilirubinemia in Gunn Rats. Int. J. Artif. Organs 14, 239–241.
Bruni, S. and Chang, T. M. S. (1995) Kinetics of UDP-glucuronosyl-transferase in bilirubin conjugation by encnapsulated hepatocytes for transplantation into Gunn rats J. Artif. Organs 19, 449–457.
Wong, H. and Chang, T. M. S. (1991) A novel two step procedure for immobilizing living cells in microcapsules for improving xenograft survival. J. Biomater. Artif. Cells Immobilization Biotechnol. 19, 687–698.
Chang, T. M. S. and Wong, H. (1992) A novel method for cell encapsulation in artificial cells. USA Patent No. 5,084,350.
Prakash, S. and Chang, T. M. S. (1995) Kinetic studies of microecnapsulated genetically engineered E. coli cells containing K. aerogenes gene for urea and ammonia removal. J. Biotechnol. Bioeng. 46, 621–626.
Prakash, S. and Chang, T. M. S. (1996) Microencapsulated genetically engineered live E. coli DH5 cells administered orally to maintain normal plasma urea level in uremic rats. Nature Med. 2, 883–887.
Koo, J. and Chang, T. M. S. (1993) Secretion of erythropoietin from microencapsulated rat kidney cells: preliminary results. Int. J. Artif. Organs 16, 557–560.
Garofalo, F. and Chang, T. M. S. (1991) Effects of mass transfer and reaction kinetics on serum cholesterol depletion rates of free and immobilized Pseudomonas pictorum. Appl. Biochem. Biotechnol. 27, 75–91.
Lyold-George, I. and Chang, T. M. S. (1995) Characterization of free and alginate-polylysine-alginate microencapsulated Erwinia herbicola for the conversion of ammonia, pyruvate and phenol into l-tyrosine and l-DOPA. J. Bioeng. Biotechnol. 48, 706–714.
Yu, Y. T. and Chang, T. M. S. (1981) Lipid-polymer membrane artificial cells containing multienzyme systems, cofactors and substrates for the removal of ammonia and urea. Trans. Am. Soc. Artif. Intern. Organs 27, 535–538.
Chang, T. M. S. (1971) Stabilization of enzyme by microencapsulation with a concentrated solution o or by crosslinking with glutaraldehyde. Biochem. Biophys. Res. Com. 44, 1531–1533.
Coromili, V. and Chang, T. M. S. (1993) Polydisperse dextran as a diffusing test solute to study the membrane permeability of alginate polylysine microcapsules. J. Biomater. Artif. Cells Immobilization Biotechnol. 21, 323–335.
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© 1999 Humana Press Inc., Totowa, NJ
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Chang, T.M.S. (1999). Microencapsulation of Enzymes, Cells, and Genetically Engineered Microorganisms. In: Morgan, J.R., Yarmush, M.L. (eds) Tissue Engineering Methods and Protocols. Methods in Molecular Medicine™, vol 18. Humana Press. https://doi.org/10.1385/0-89603-516-6:315
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DOI: https://doi.org/10.1385/0-89603-516-6:315
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