Part of the Methods in Molecular Medicine™ book series (MIMM, volume 18)
Methods for Microencapsulation with HEMA-MMA
Encapsulation of cells in a membrane prior to implantation holds potential for controlling the adverse immune response that may be generated against the transplanted cells, by physically isolating the cells from the host’s immune system. If successful, encapsulation eliminates or minimizes the adverse effects of immunosuppressive therapy and permits the use of xenogeneic cells. Ideally, the capsule membrane holds permselective properties, so that the passage of nutrients, growth factors, and the therapeutic product secreted by the cells occur readily across the membrane, but mediators of the immune system do not penetrate the membrane (Fig. 1). The major types of immunoisolation devices include intravascular arteriovenous shunts, diffusion chambers of tubular or planar geometry, and microcapsules (1, 2, 3, 4, 5, 6).
KeywordsCore Needle Polyethylene Tubing Tygon Tubing Core Solution Silastic Tubing
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