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Culture and Identification of Autologous Human Articular Chondrocytes for Implantation

  • Ross Tubo
  • Francois Binette
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 18)

Abstract

The disability and pain that result from damage to articular cartilage within the knee joint has stimulated the development of several approaches to facilitate the restoration of joint function (1, 2, 3, 4, 5, 6, 7, 8, 9). Recently, cultured autologous chondrocytes, isolated from an individual’s own cartilage, have been expanded in vitro, and then implanted into the damaged site for repair of damaged knee cartilage (10). This remarkable process has been characterized by the modulation of gene expression during proliferation expansion and subsequent redifferentiation of cultured chondrocytes in vitro (11) and in vivo (12). Since the unique biomechanical properties of hyaline articular cartilage have been shown to be intimately linked with the biochemistry of the tissue (see Buckwalter and Mow ref. 13 for review), we have developed an in vitro system to verify that proliferatively expanded chondrocytes retain their ability to redifferentiate, or re-express their hyaline articular cartilage phenotype. Although the methods described herein were developed for specific application to chondrocytes, the principles for evaluation of biochemical and molecular biological properties of tissue-engineered materials, in vitro, may be applied to the development of any functional, high quality, tissue engineered implant.

Keywords

Articular Cartilage RNase Protection Assay Calcium Chloride Solution Collagen mRNA Digestion Vessel 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 1999

Authors and Affiliations

  • Ross Tubo
    • 1
  • Francois Binette
    • 1
  1. 1.Genzyme Tissue Repair, Inc.Framingham

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