Skip to main content
SpringerLink
Log in

Recombinant Baculoviruses

  • Protocol
Biopesticides: Use and Delivery

Part of the book series: Methods in Biotechnology ((MIBT,volume 5))

  • 1829 Accesses

Abstract

Baculovindae is a family of occluded, invertebrate-specific pathogens, consisting of two generathe nucleopolyhedroviruses (NPVs) and granuloviruses. The majority of basic and applied research efforts, as well as commercial endeavors, have been focused on NPVs. In addition to vertebrate—invertebrate selectivity, many NPVs are infectious against only certain species within the insect order Lepidoptera, and impart no direct adverse effects on members of other insect orders, such as Coleoptera, Hymenoptera, Neuroptera, and Diptera (1). Examples of lepidopteran-specific baculoviruses are those isolated from gypsy moth, Lymantria dispar (LdNPV), celery looper, Anagrapha falcifera Kirby (AfNPV), beet armyworm, Spodoptera exigua (Hubner) (SeNPV), and cotton bollworm, Helicoverpa zea (Boddie) (HzNPV) Target specificity of NPVs make them good candidates for use in integrated pest management systems.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Bishop, D. H L, Entwistle, P F, Cameron, I. R, Allen, C J, and Possee, R D (1988) Field trial of genetically engineered baculovirus insecticides, in The Release of Genetically Engineered Organisms(Susman, M., Collins, C H., Skin ner, F A., and Stewart-Tull, D E., eds.), Academic, New York,. 143–179

    Google Scholar 

  2. O’Reilly, D. R. and Miller, L K. (1989) A baculovirus blocks molting by produc ing ecdysteroid UDP-glucosyltransferase. Science 245, 1110–1112

    Article  PubMed  Google Scholar 

  3. O’Reilly, D R. and Miller, L. K (1991) Improvement of a baculovirus pesticide by deletion of the egt gene Bio/Technology 9, 1086–1089

    Article  Google Scholar 

  4. O’Reilly, D. R. (1995) Baculovirus-encoded ecdysteroid UDP-glucosyltransferases. Insect Bwchem Mol Biol 25, 541–550

    Article  Google Scholar 

  5. Riegel, C I, Park, E., Burand, J, and Slavicek, J (1994) Deletion of the Lymantria dispar multicapsid_nuclear polyhedrosis virus EGT gene enhances viral killing speed General TechnicalReport NE-188, USDA Interagency Gypsy Moth Research Forum, Beltsville, MD

    Google Scholar 

  6. Clarke, E E., Tnstem, M., Cory, J S, and O’Reilly, D R. (1996) Characteriza tion of the ecdysteroid UDP-glucosyltransferase gene from Mamestra brassicaeucleopolyhedrovirus. J Gen Virol 11, 2865–2871

    Article  Google Scholar 

  7. Faktor, O, Toister-Achituv, M., and Kamensky, B (1995) Identification and nucleotide sequence of an ecdysteroid UDP-glucosyltransferase gene of Spo doptera httorahs multicapsid_nuclear polyhedrosis virus Virus Genes 11, 47–52

    Article  PubMed  CAS  Google Scholar 

  8. Hu, Z. H, Broer, R., Westlaken, J, Martens, J W M., Jin, F, Jehle, J A, Wang, L M, and Vlak, J. M. (1997) Characterization of ecdysteroid UDP-gluco syltransferase gene of a single nucleocapsid nucleopolyhedrovirus of Buzura suppressaria Virus Res 47, 91–97

    Article  CAS  Google Scholar 

  9. Treacy, M. F, All, J N, and Ghidiu, G. M (1997) Impact of ecdysteroid UDPglucosyltransferase gene deletion on efficacy of a baculovirus against Hehothis virescens and Trichoplusia ni J Econ Entomol, _press

    Google Scholar 

  10. Carbonell, L F, Hodge, M. R., Tomalski, M. D., and Miller, L K. (1988) Synthe sis of a gene coding for an insect-specific scorpion neurotoxin and attempts to express it using baculovirus vectors. Gene 73, 409–418.

    Article  PubMed  CAS  Google Scholar 

  11. Maeda, S. (1989) Increased insecticidal effect of a recombinant baculovirus carry ing a synthetic diuretic hormone. Bwchem Biophys Res Commun 165, 1177–1183

    Article  CAS  Google Scholar 

  12. Hammock, B. D., Bonning, B C, Possee, R D., Hanzlik, T N, and Maeda, S (1990) Expression and effects of juvenile hormone esterase in a baculovirus vec tor. Nature 344, 458–461

    Article  CAS  Google Scholar 

  13. Bonning, B C and Hammock, B D. (1996) Development of recombinant baculovirus for insect control Ann. Rev Entomol 41, 191,210

    Article  Google Scholar 

  14. Zlotkin, E., Rochat, H, Kopeyan, C, Miranda, F, and Lissitzky S (1971) Purifi cation and properties of the insect toxin from venom of the scorpion Androctonus australis Biochemie 53, 1073–1078.

    CAS  Google Scholar 

  15. Walther, C, Zlotkin, E., and Rathmayer, W (1976) Action of different toxins from the scorpion Androctonus australis on a locust nerve-muscle preparation. J Insect Physiol 22, 1187–1194

    Article  Google Scholar 

  16. Teitelbaum, Z., Lazarovici, P., and Zlotkin, E. (1979) Selective binding of scor pion venom insect toxin to insect nervous tissue. Insect Bwchem 9, 343–346

    Article  CAS  Google Scholar 

  17. Zlotkin, E. (1986) The interaction of insect selective neurotoxins from scorpion venoms with insect neuronal membranes, in Neuropharmacology and Pesticide Action (Ford, M. G, Lunt, G. G., Reay, R. C, and Usherwood, P N. R, eds), Ellis Horwood, Chichester, UK, 352–383.

    Google Scholar 

  18. McCutchen, B F, Choudary, P. V. Crenshaw, R., Maddox, D., Kamita, S G, Pelekar, N, et al (1991) Development of a recombinant baculovirus express ing an insect selective neurotoxin: potential for pest control. Bio/Technology 9, 848–852

    Article  PubMed  CAS  Google Scholar 

  19. Steward, L M D., Hirst, M, Ferber, M L, Merryweather, A T, Cayley, P J, and Possee, R. D (1991) Construction of an improved baculovirus insecticide containing an insect-specific toxin gene. Nature 352, 85–88

    Article  Google Scholar 

  20. Treacy, M. F. and All, J. N. (1996) Impact of insect-specific AaHIT gene inser tion on inherent bioactivity of baculovirus against tobacco budworm, Hehothis virescens, and_cabbage looper, Trichoplusia m, in Proceedings, Beltwide Cotton Conferences (Duggar P and Richter, D. A., eds), National Cotton Council, Mem phis, TN, 911–917

    Google Scholar 

  21. Maeda, S, Volrath, S L, Hanzlik, T N, Harper, S A, Majima, K, Maddox, D. W, Hammock, B. D, and Fowler, E. (1991) Insecticidal effects of an insect-specific neurotoxin expressed by a recombinant baculovirus. Virology 184, 777–780.

    Article  PubMed  CAS  Google Scholar 

  22. Kunimi, Y, Fuxa, J R, and Hammock, B D (1996) Comparison of wild type and genetically engineered nuclear polyhedrosis viruses of Autographa califonica for_mortality, virus replication and polyhedra production in Trichoplusia nilar vae Entomol Exp Appl 81, 251–257.

    Article  Google Scholar 

  23. Zlotkin, E, Eitan, M., Bindokas, M. E, Adams, M E, Moyer, M, Burkhart, W, and Fowler, E. (1991) Functional duality and structural uniqueness of depressant insect-selective neurotoxins Biochemistry 30, 4814–4821.

    Article  PubMed  CAS  Google Scholar 

  24. Tomalski, M. D, Kutney, R., Bruce, W A, Brown, M R, Blum, M S, and Travis, J (1989) Purification and characterization of insect toxins derived from the mite Pymotes tritici Toxicon 27, 1151–1167

    Article  CAS  Google Scholar 

  25. Tomalski, M. and Miller, L. K. (1991) Insect paralysis by baculovirus mediated expression of a mite neurotoxin gene Nature 352, 82–85

    Article  PubMed  CAS  Google Scholar 

  26. DuPont Agricultural Products (1996) Notification to conduct small-scale field testing of a genetically engineered microbial pesticide (no_352-NMP-U), in Federal Register, United States Environmental Protection Agency, Washington, DC, 61, 24,934–24,935.

    Google Scholar 

  27. Prikhod’ko, G. G, Robson, M., Warmke, J. W, Cohen, C. J., Smith, M M., Wang, P, et al (1996) Properties of three baculovirus-expressing genes that encode insect-selective toxins 03B1-Aga-IVB, AsII, and ShI Biol Control 7, 236–244

    Article  Google Scholar 

  28. Hughes, P R, Wood, H A, Breen, J P, Simpson, S F, Duggan, A. J, and Dybas, J A (1997) Enhanced bioactivity of recombinant baculoviruses express ing insect-specific spider toxins in lepidopteran crop pests. J Invert Pathol 69, 112–118.

    Article  Google Scholar 

  29. Shih, C J and Hu, Y C. (1996) The insecticidal activity of genetically engi neered Autographa californica nuclear_polyhedrosis virus containing the Bacillus thuringiensis toxin gene to Sf9 cell line and Spodoptera litura larvaeZhongua Kunchong 16, 1–12

    Google Scholar 

  30. Hughes, P R. and Wood, H. A (1986) In-vivo and in-vitro bioassay methods for baculoviruses, in The Biology of Baculoviruses, Practical Application for Insect Control (Granados, R R and Federici, B A, eds.), CRC, Boca Raton, FL, 1–26

    Google Scholar 

  31. Teakle, R. E, Jensen, J M, and Giles, J E (1986) Age related susceptibility of Heliothis punctigera to a commercial formulation of nuclear polyhedrosis virus J Invert Pathol 47, 82–92.

    Article  Google Scholar 

  32. Santiago-Alvarez, C and Ortiz-Garcia, R (1992) The influence of host plant on the susceptibility of Spodoptera littoralis larvae to Spodoptera littoralis NPV. J Appl Entomol 114, 124–130

    Article  Google Scholar 

  33. Forschler, B. T, Young, S Y., and Felton, G W. (1992) Diet and susceptibility of Hehcoverpa zea to a nuclear polyhedrosis virus. Environ Entomol 21, 1220–1223

    Google Scholar 

  34. Felton, G. W and Duffey, S S (1990) Inactivation of a baculovirus by quinones formed in an insect damaged plant tissue J Chem Ecol 16, 1211–1236.

    Google Scholar 

  35. Hunter, M D and Schultz, J C (1993) Induced plant defenses breached phytochemical induction protects an herbivore from disease Oecologica 94, 195–203

    Article  Google Scholar 

  36. Duffey, S S, Hoover, K, Bonning, B C, and Hammock, B D (1995) The impact of host plant on the efficacy of baculoviruses, in Reviews in Pesticide Toxicology (Roe, M and Kuhr, R, eds), CTI Toxicology Communications, Raleigh, NC, 137–275.

    Google Scholar 

  37. Young, S Y, Yang, J G, and Felton, G W (1995) Inhibitory effects of dietary tanins on infectivity of a nuclear polyhedrosis virus to Hehcoverpa zea (Noctuidae: Lepidoptera) Biol Control 5, 145–150

    Article  Google Scholar 

  38. Jones, K A (1988) PhD Thesis, University of Reading, Chatham, Kent, UK.

    Google Scholar 

  39. Hoover, K, Schultz, C. M, Lane, S S., Bonning, B C, Hammock, B D, and Duffey, S. S (1997) Effects of diet-age and streptomycin on virulence of Autographa californica nucleopolyhedrovirus against tobacco budworm J Invert Pathol 69, 46–50.

    Article  CAS  Google Scholar 

  40. Jarvis, D L, Reilly, L M, Hoover, K, Schultz, C, Hammock, B D., and Guarino, L. A. (1996) Construction and characterization of immediate early baculovirus pesticides Biol Control 7, 228–235.

    Article  Google Scholar 

  41. Hoover, K, Schultz, C M, Lane, S. S., Bonning, B C, Duffey, S S., McCutchen, B F, and Hammock, B. D (1995) Reduction in damage to cotton plants by a recombinant baculovirus that knocks moribund larvae of Heliothis virescens off the_plant. Biol Control 5, 419–426

    Article  Google Scholar 

  42. Cory, J. S., Hirst, M. L., Williams, T, Haik, R. S., Goulson, D, Greer, B. M, et al (1994) Field trial of a genetically improved baculovirus insecticide Nature 370, 138–140.

    Article  Google Scholar 

  43. Treacy, M F. (1997) Efficacy and non-target arthropod safety of an AalT geneinserted baculovirus. results from field and laboratory studies conducted during 1995-1996, in Biopesticides and Transgenic Plants, International Business Com munications, Southborough, MA, in press.

    Google Scholar 

  44. Bishop, D. H. L., Hirst, M L, Possee, R. D, and Cory, J. S (1995) Genetic engi neering of microbes’ virus insecticides-a case study, inFifty Years of Antimicrobi als Past Perspectives and Future Trends (Hunter, P. A., Darby, G. K, and Russell, N. J., eds), Cambridge University Press, Cambridge, UK, 249–277

    Google Scholar 

  45. Heinz, K M, McCutchen, B F, Hermann, R., Parella, M P, and Hammock, B D. (1995) Direct effects of recombinant nuclear polyhedrosis viruses on selected nontarget organisms. J Econ Entomol 88, 259–264

    PubMed  CAS  Google Scholar 

  46. McCutchen, B F, Hermann, R., Heinz, K M., Parella, M P, and Hammock, B D (1996) Effects of recombinant baculoviruses on a nontarget endoparasitoid of Heliothis virescens Biol Control 6, 45–50.

    Article  Google Scholar 

  47. Treacy, M F., All, J N, and Kukel, C. F (1997) Invertebrate selectivity of a recombinant baculovirus’ case study on AaHIT gene-inserted Autographa cahfornica nuclear polyhedrosis virus, in New Developments in Entomology (Bondan, K, ed), Research Signpost, Trivandrum, India, 57–68.

    Google Scholar 

  48. Jaques, R P (1968) The inactivation of the nuclear polyhedrosis virus of Trichoplusia ni by gamma and ultraviolet radiation. Can J Microbiol. 14, 1161–1163

    Article  PubMed  CAS  Google Scholar 

  49. Jaques, R P (1977) Stability of entomopathogenic viruses, in Environmental Sta bility of Microbial Insecticides (Ignoffo, C. M. and Hostetter, D. L., eds), Misc Publ. Entomol. Soc Am 10, 99–116.

    Google Scholar 

  50. Jones, K A., Moawad, G, McKinley, D. J., and Grzywacz, D. (1993) The effect of natural sunlight on Spodoptera littoralis nuclear polyhedrosis virus. Biocontrol Sci Technol 3, 189–197

    Article  Google Scholar 

  51. Jaques, J. P (1969) Soil stability of baculoviruses. J Invert Pathol. 13, 256–263

    Article  Google Scholar 

  52. Ignoffo, C M. and Batzer, O. F. (1971) Microencapsulation and ultraviolet protectants to increase sunlight stability of an insect virus J Econ Entomol 64, 850–853

    CAS  Google Scholar 

  53. Jones, K A and McKinley, D. J (1987) Persistence of Spodoptera httorahs

    Google Scholar 

  54. Ignoffo, C. M, Hostetter, D. L, Sikorowski, R. P., Sutter, G., and Brooks, W M (1977) Inactivation of respective species of entomopathogenic viruses, a bacterium, fungus, and a protozoan by ultraviolet light source. Environ Entomol 6, 411–415

    Google Scholar 

  55. Ignoffo, C. M, Rice, W. C, and Mcintosh, A H. (1989) Inactivation of nonoccluded and occluded baculoviruses and baculovirus-DNA exposed to simu lated sunlight. Environ Entomol 18, 177–183

    Google Scholar 

  56. Ignoffo, C M and Garcia, C (1992) Combinations of environmental factors and simulated sunlight affecting activity of inclusion bodies of the Heliothis

    Google Scholar 

  57. Tomalski, M. and Miller, L. K. (1991) Expression of a paralytic neurotoxin gene to improve insect baculoviruses as biopesticides. Bio/Technology 10, 545–549

    Article  Google Scholar 

  58. American Cyanamid (1994) Notification to conduct small-scale field testing of a genetically altered baculovirus (no. 241-NMP), in Federal Register, United States Environmental Protection Agency, Washington, DC

    Google Scholar 

  59. Ignoffo, C. and Garcia, C (1996) Rate of larval lysis and yield and activity of inclusion bodies harvested from Trichoplusia ni larvae fed a wild or recombinant strain of the nuclear polyhedrosis virus of Autographa californica J Invert Pathol 68, 196–1

    Article  Google Scholar 

  60. Goulson, D. (1997) Modification of host behavior during baculoviral infection Oecologia 109, 219–228

    Article  Google Scholar 

  61. Lu, A, Seshagiri, S, and Miller, L K. (1996) Signal sequence and promoter effects on the efficacy of toxin-expressing baculoviruses as biopesticides Biol Control 7, 320–332.

    Article  Google Scholar 

  62. DuPont Agricultural Products (1997) Notification to conduct small-scale field testing of genetically engineered microbial pesticides (no. 352-NMP-004), in Federal Register, United States Environmental Protection Agency, Washington, DC, 62, 23,448–23,449.

    Google Scholar 

  63. American Cyanamid (1997) Notification to conduct small-scale testing of a genetically engineered microbial pesticide, in Federal Register, United States Environmental Protection Agency, Washington, DC, 62, 39,518

    Google Scholar 

  64. Thiem, S M, Du, X, Qentin, M. E., and Berner, N M (1996) Identification of a baculovirus gene that promotes Autographa cahformca nuclear polyhedrosis virus replication in a nonpermissive insect cell line J Virol 70, 2221–2229

    PubMed  CAS  Google Scholar 

  65. Du, X and Thiem, S. M. (1997) Characterization of host range factor 1 (hrf-1) expression in Lymantria dispar nucleopolyhedrovirus-and recombinant Auto grapha californica nucleopolyhedrovirus-infected IPLB-Ld652Y Virology 227, 420–430

    Article  PubMed  CAS  Google Scholar 

  66. Hermann, R., Moskowitz, H, Zlotkin, E., and Hammock, B D (1995) Positive cooperativity among insecticidal scorpion toxins Toxicon 33, 1099–1102

    Article  Google Scholar 

  67. All, J N and Treacy, M. F. (1997) Improved control of Helwthis virescens and Helicoverpa zea with a recombinant form of Autographa californica nuclear poly hedrosis virus and interaction with Bollgard® cotton, in Proceedings Beltwide Cotton Conferences (Duggar, P and Richter, D. A., eds.), National Cotton Coun cil, Memphis, TN, 1294–1296.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Amerctan Cyanamid Co., Prtnceton, NJ

    Michael F. Treacy

Authors
  1. Michael F. Treacy
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Ohio State University, Wooster, OH

    Franklin R. Hall

  2. Excipula Inc, Highland, MD

    Julius J. Menn

Rights and permissions

Reprints and permissions

Copyright information

© 1999 Humana Press, Totowa, NJ

About this protocol

Cite this protocol

Treacy, M.F. (1999). Recombinant Baculoviruses. In: Hall, F.R., Menn, J.J. (eds) Biopesticides: Use and Delivery. Methods in Biotechnology, vol 5. Humana Press. https://doi.org/10.1385/0-89603-515-8:321

Download citation

  • DOI: https://doi.org/10.1385/0-89603-515-8:321

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-515-7

  • Online ISBN: 978-1-59259-483-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation