Abstract
The vast majority of somatic cells possess the same complement of genes. What makes one cell different from another is the subset of genes that each expresses. Further, the regulated expression of different sets of genes within a cell largely controls phenomena such as cell development, differentiation, adaptive responses to environmental signals (including drug treatment), and even certain types of cell death.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Bateson, A N and Tanay, V A-M I (1999) Multiprobe oligonucleotide solution hybridization for the determination of relative mRNA levels, inNeuromethods, vol 33 Molecular Neurobiology Techniques (Boulton, A A, Baker, G B, and Bateson, A N, eds) Humana, Totowa, NJ, pp 101–126
Bauer, D, Muller, H, Reich, J, Rledel, H, Ahrenkiel, V., Warthoe, P., and Strauss, M (1993) Identificatlon of differentially expressed mRNA species by an improved display technique (DDRT-PCR) Nucleic Acids Res 21, 4272–4280
Clinton, M., and Scougall, R K (1995) Detection and capture of 35S-labeled gas released from reaction tubes during differential display PCR. Biotechnology 19, 798,799
Dalal, S. S, Welsh, J, Tkachenko, A, Ralph, D, DiClcco-Bloom, E, Bordas, L, McClelland, M., and Chada, K (1994) Rapid isolation of tissue-specific and developmentally regulated brain cDNAs using RNA arbitrarily primed PCR (RAP-PCR) J Mol Neurosci 5, 93–104
Dou, D and Joseph, R (1996) Cloning of human neuronatin gene and its localization to chromosome-20qll 2–12 the deduced protein is a novel ‘proteolipid’Brain Res 723 8–22
Douglass, J and Daoud, S (1996) Characterization of the human cDNA and genomic DNA encoding CART a cocaine-and amphetamine-regulated transcriptGene 169, 241–245
Douglass, J, McKinzie, A A, and Couceyro, P (1995) PCR differential display identifies a rat brain mRNA that is transcriptionally regulated by cocaine and amphetamine J. Neurosci 15, 2471–2481
Godbout, R and Andison, R (1996) Elevated levels of cyclin D1 mRNA in the undifferentiated chick retina Gene 182, 111–115
Grayson, D. R, and Ikonomovic, S (1999) Competitive RT-PCR to quantrtate receptor subunit mRNA levels, in Neuromethods, vol 33 Molecular Neurobiology Techniques (Boulton, A A, Baker, G B, and Bateson, A N, eds) Humana, Totowa, NJ, pp 127–151
Kiryu, S, Yao, G L, Morita, N, Kato, H., and Klyama, H (1995) Nerve injury enhances rat neuronal glutamate transporter expression identification by differential display J Neurosci 15, 7872–7878
Kondo, T, Shiasawa, T, Itoyama, Y, and Mori, H (1996) Embryonic genes expressed in Alzhelmer’s disease brains Neurosci Lett 209, 157–160
Liang, P, Averboukh, L, and Pardee, A B (1993) Distribution and cloning of eukaryotlc mRNAs by means of differential display refinements and optlmization Nucleic Acids Res 21, 3269–3275
Liang, P, Bauer, D, Averboukh, L, Warthoe, P, Rohrwild, M, Muller, H., Strauss, M, and Pardee, A B (1995) Analysis of altered gene expression by differential display Meth Enzymol 254, 304–321.
Liang, P and Pardee, A B. (1992) Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction Science 257, 967–971
Livesey, F J and Hunt, S P (1996) Identifying changes in gene expression in the nervous system mRNA differential displayTINS 19, 84–88.
Marechal, D., Forcellle, C, Breyer, D, Delapierre, D, and Dresse, A (1993) A subtractive hybridization method to isolate tissue-specific transcripts application to the selection of new brain-specific products Analytical Biochem 208, 330–333
Maser, R L and Calvet, J P (1995) Analysis of differential gene expression in the kidney by differential cDNA screening, subtractive cloning, and mRNA differential display Seminars Nephrol 15, 29–42
Masiakowski, P, Breathnach, R, Bloch, J, Gannon, F, Krust, A., and Chambon, P. (1982) Cloning of cDNA sequences of hormone-regulated genes from the MCF-7 human breast cancer cell line. Nucleic Acids Res 10, 7895–7903
Morita, N., Kiryu, S, and Kiyama, H. (1996) p53-independent cyclin G expression in a group of mature neurons and its enhanced expressron during nerve regeneration J Neusrosci 16, 5961–5966.
Mou, L., Miller, H, Li, J, Wang, E, and Chalifour, L (1994) Improvements to the differential display method for gene analysis Biochem Biophys Res Comm 199, 564–569
Nedivi, E, Hevroni, D, Naot, D, Israeli, D, and Citrl, Y (1993) Numerous candidate plasticity-related genes revealed by differential cDNA cloningNature 363, 718–722
O’Farrell, P H (1975) High resolutron two-dimensional electrophoresls of proteinsJ Biol Chem 250, 4007–4021
Rothstem, J L., Johnson, D, Jessee, J, Skowronski, J, DeLoia, J. A, Solter, D,and Knowles, B B (1993) Construction of primary and subtracted cDNA libraries from early embryos. Meth. Enzymol 225, 587–610
Uchiyama, C M, Zhu, J, Carroll, R S, Leon, S P, and Black, P M (1995)Differential display of messenger rrbonucleic acid a useful technique for analyzing differential gene expression in human bram tumors Neurosurgery 37, 464–470
Vellucci, V. F., Germino, F J., and Reiss, M (1995) Cloning of growth regulatory genes from primary human keratinocytes by subtractive hybridlzationGene 166, 213–220
Vogeli-Lange, R, Buckert, N, Boller, T, and Wiemken, A (1996) Rapid selection and classrficatlon of positive clones generated by mRNA differential display. Nucleic Acids Res 24, 1385,1386
Wang, X, Yue, T. L, Barone, F C, White, R F, Clark, R K, Wrllette, R N, Sulpizro, A C, Aiyar, N V, Ruffolo, R. R, and Feuerstein, G Z (1995) Discovery of adrenomedullin in rat lschemic cortex and evidence for its role in exacerbating focal bram ischemic damage Proc Natl Acad Sci USA 92, 11,480-l1,484.
Watson, J. B. and Margulies, J E. (1993) Differential cDNA screeing strategie to identify novel stage-specific proteins in the developing mammalian brainDev Neurosci 15, 77–86
Welsh, J., Chada, K., Dalal, S S, Cheng, R., Ralph, D, and McClelland, M (1992)Arbritarily primed PCR fingerprinting of RNA. Nucleic Acids Res 20, 4965–4970
Wong, M-L, Khatri, P., Licinio, J, Esposito, A., and Gold, P W (1996) Identification of thalamic transcripts upregulated by antidepressants Biochem Biophys Res Comm 229, 275–279
Yee, F and Yolken, R. H ( 1997) Identification of differentially expressed RNA transcripts in neuropsychiatric disorders Biol Psychiatry 41, 759–761
Zhang, H., Zhang, R, and Liang, P (1996) Differential screening of gene expression difference enriched by differential display Nucleic Acids Res 24, 2454,2455
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Humana Press Inc
About this protocol
Cite this protocol
Tanay, V.AM., Bateson, A.N. (1999). Messenger RNA Differential Display. In: Boulton, A.A., Baker, G.B., Bateson, A.N. (eds) In Vitro Neurochemical Techniques. Neuromethods, vol 34. Humana Press. https://doi.org/10.1385/0-89603-509-3:75
Download citation
DOI: https://doi.org/10.1385/0-89603-509-3:75
Publisher Name: Humana Press
Print ISBN: 978-0-89603-509-6
Online ISBN: 978-1-59259-639-3
eBook Packages: Springer Protocols