Abstract
Allogeneic bone marrow transplantation (BMT) has become a recognized therapy for the treatment of patients with leukemia, severe combined immune deficiency (SCID) and severe aplastic anemia (SAA). It was originally assumed that complete donor chimerism was essential for sustained engraftment. However, despite high dose chemoradiotherapy given as preconditioning, the persistence of host hematopoietic cells (mixed chimerism) has commonly been observed after BMT and the relationships between mixed chimerism, engraftment and relapse have remained controversial (1–4). To evaluate the clinical relevance of mixed chimerism after BMT, we developed a method based on the amplification of minisatellite DNA regions by the polymerase chain reaction (PCR) (5,6).
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© 1998 Humana Press Inc., Totowa, NJ
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Roux, E. (1998). PCR Amplification of Minisatellite DNA for the Detection of Mixed Chimerism After Bone Marrow Transplantation. In: Lo, Y.M.D. (eds) Clinical Applications of PCR. Methods in Molecular Medicine™, vol 16. Humana Press. https://doi.org/10.1385/0-89603-499-2:161
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DOI: https://doi.org/10.1385/0-89603-499-2:161
Publisher Name: Humana Press
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