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The Application of Molecular Techniques for the Study of Aminoglycoside Resistance

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Molecular Bacteriology

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 15))

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Abstract

Aminoglycosides have been cinically used since 1944. Although this class of antibacterial agents has some nephrotoxicity and ototoxtcity issues, they continue to be part of the hospital armamentarium because of then rapid bactericidal activity, especially in combination with β-lactams. Bacterial resistance to aminoglycosides can be caused by modifying enzymes, changes in cell permeability, and changes in the cellular target. The clinical observation of high levels of aminoglycoside resistance most often results from the acquisition of genes that encode modifying enzymes and are often plasmid-borne. Aminoglycosides are inactivated by three classes of enzymes:

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References

  1. Davres, J and Smith, D I(1978) Plasmid-determined resistance to antimicrobial agents. Ann Rev Mlcrobzol 32, 469–518.

    Article  Google Scholar 

  2. Foster, T J (1983) Plasmid-determined reststance to antimtcrobial drugs and toxic metal ions in bacteria Mzcrobzol. Rev 47, 361–4

    CAS  Google Scholar 

  3. Shaw, K J, Rather, P. N, Hare, R.S., and Miller, G H(1993) Molecular genetics of aminoglycoside resrstance genes, and familial relationships of the aminoglycoside-modifying enzymes.Mwrobzol Rev 57, 138–163

    CAS  Google Scholar 

  4. Miller, G H, Sabatelli, F.J Naples, L. Hare, R.S. Shaw, K.J., and The Aminoglycoside Resistance Groups (1995) The most frequently occuring aminoglycoside resistance mechanisms-combined results of surveys in eight regions of the world.J Chemother 7 suppl. 2, 17–30

    Google Scholar 

  5. Shaw, K J, Hare, R S, Sabatelli, F J, Rtzzo, M, Cramer, C.A., Naples, L, Kocsi, S., Munayyer, H., Mann, P, Miller, G.H, Verbist, L, Landuyt, H V., Glupczynski, Y, Catalano, M., and Woloj, M(1991) Correlation between aminoglycoside resistance profiles and DNA hybridization of clinical isolates. Antlmicrob Agents Chemother 35, 2253–2261

    CAS  Google Scholar 

  6. Miller, G. H., Sabatelli, F J, Naples, L., Hare, R. S, Shaw, K.J., and The Amtnoglycosrde Resistance Study Groups (1995) The changing nature of aminoglycoside resistance mechanisms and the role of isepamicin-a new broadspectrum aminoglycoside. J Chemother 7 suppl. 2, 3I–44.

    Google Scholar 

  7. Shaw, K J, Munayyer, H, Rather, P.N., Hare, R.S., and Miller, G.H(1993)Nucleotide sequence analysis and DNA hybndization studies of the ant(4I)-IIu gene from Pseudomonas aeruginosa. Antimicrob Agents Chemother 37, 708–714.

    CAS  Google Scholar 

  8. Martin, P., Jullien, E,and Courvalin, P.(1988)Nucleotide sequence of Acinetobacter baumannii aphA-6 gene: evolutionary and functional implications of sequence homologies with nucleotide-binding proteins, kinases and other aminoglycoside-modifying enzymes. Mel Mlcroblol 2, 615–625

    Article  CAS  Google Scholar 

  9. Van de Klundert, J.A.M., Gestal, M H.V, Vhiegenthart, J S,and Ketelaar-Van Gaalen, P.A.G.(1992) Aminoglycoside-resistance genes in coagulase-negative staphylocci as detected by PCR. Abstr Ann Meeting, ICAAC #700

    Google Scholar 

  10. Rather, P. N., Orosz, E., Shaw, K.J., Hare, R.,and Miller, G.(1992) Genetic analysis of the 2’-N-acetyltransferase from Prowdencia stuartll. Abstr. Ann Meeting, ICAAC #436

    Google Scholar 

  11. Vhegenthart, J S, Ketelaar-van Gaalen, P A.G,and ptvan de Klundert, J A.M.(1991) Nucleotide sequence of the aacC3 gene, a gentamicin resistance determinant encoding aminoglycoside-(3)-N-acetyltransferase III expressedin Pseudomonas aeruginosa, but not in Escherzchta coli. Antimzcrob Agents Chemother 35, 892–897.12 Chaslus-Dancla, E., Glupczynski, Y, Gerbaud, G., Lagorce, M, Lafont, J. P., and Courvain, P. (1989) Detection of apramycin resistant Enterobacteriaceae in hospital isolates. FEMS Microbiol Lett. 61,261-266#@#13. Rather, P. N., Mann, P, Mierzwa, R, Hare, R. S,, Miller, G. H., and Shaw, K J (1993) Analysis of the sac(3)-VZa gene encoding a novel 3-N-acetyltransferase Antlmlcrob Agents Chemother 37, 2074-2079.

    Google Scholar 

  12. Chaslus-Dancla, E., Glupczynskl, Y , Gerbaud, G., Lagorce, M , Lafont, J. P., and Courvalin, P. (1989) Detection of apramycm resistant Enterobacteriaceae in hospital isolates. FEMS Mzcrobzol Lett. 61, 261–266

    Article  CAS  Google Scholar 

  13. Rather, P. N., Mann, P , Mlerzwa, R , Hare, R. S,, Miller, G. H., and Shaw, K J (1993) Analysis of the aac(3)-Vla gene encoding a novel 3-N-acetyltransferase Antimicrob Agents Chemother 37,2074–2079.

    PubMed  CAS  Google Scholar 

  14. Ploy, M.C., Giamarellou, H, Bourlioux, P, Courvalin, P, and Lambert, T (1994) Detection of aac(61)-I genes in amikacin-resistant Acinetobacter spp by PCR Antlmicrob Agents Chemother 38, 2925–2928.

    CAS  Google Scholar 

  15. Lambert, T., Gerbaud, G., and Courvalin, P.(1994) Characterization of the chromosomal aac(6′)-IJ gene of Acinetobacter sp 13 and the aac(6’)-Ih plasmid gene of Acinetobacter baumannii Antimicrob. Agents Chemother 38, 1883–1889.

    Google Scholar 

  16. Costa, Y, Galimand, M, Leclercq, R., Duval, J,and Courvalin, P(1993) Characterization of the chromosomal aac(6’)-I1 gene specific for Enterococcus faecium Antimicrob Agents Chemother 37, 1896–1903

    CAS  Google Scholar 

  17. Rudant, E., Bourlioux, P., Courvalin, P, and Lambert, T.(1994) Characterization of the aac(61)-Ik gene of Acinetobacter sp. 6.FEMS Mlcrobiol Lett. 124, 49–54

    CAS  Google Scholar 

  18. Bunny, K L, Hall, R M,and Stokes, H.W.(1995) New mobile gene cassettes containing an aminoglycoside resistance gene, aacA7, and a chloramphenicol resistance gene, catB3,in an integron in pBW301 Antimicrob Agents Chemother 39, 686–693.

    PubMed  CAS  Google Scholar 

  19. Ounissi, H. and Courvalin, P (1987) Nucleotide sequence of the gene aadE encoding the 6-streptomycin adenylyltransferase in Enterococcus faecalis,in Streptococcal Genetics, (Ferretti, J. J. and Curtiss, R. III, eds.), American Society for Microblology, Washington D C., 275.

    Google Scholar 

  20. Snelling, A M., Hawkey, P. M., Heritage, J, Downey, P., Bennett, P. M, and Holmes, B (1993) The use of a DNA probe and PCR to examine the distribution of the aac(63-Ic gene in Serratia marcescens and other Gram-negative bacteria J. Antimicrob. Chemother 31, 841–854

    Article  PubMed  CAS  Google Scholar 

  21. Vanhoof, R., Content, J, Bossuyt, E V., Dewit, L., and Hannecart-Pokorni, E (1992) Identification of the aadB gene coding for the aminoglycoside-2“-O-nucleotidyltransferase, ANT(2”), by means of the polymerase chain reaction J. Antzmicrob Chemother. 29, 365–374

    Article  CAS  Google Scholar 

  22. Vanhoof, R, Content, J., Bossuyt, E. V., Nulens, E., Sonck, P., Depuydt, F., Hubrechts, J. M., Maes, P, and Hannecart-Pokorni, E(1993) Use of the polymerasechain reaction (PCR) for the detection of aacA genes encoding aminoglycoside-6’-N-acetyltransferases in reference strains and Gram-negative clinical isolates from two Belgium hospitals.J. Antimicrob. Chemother, 32, 23–35.

    Article  PubMed  CAS  Google Scholar 

  23. Vliegenthart, J. S., Ketelaar-Van Gaalen, P. A.G., and van de Klundert, J. A. M. (1990) Identification of three genes coding for aminoglycoside-modifying enzymes by means of the polymerase chain reaction. J. Antimicrob. Chemother.25, 759–765.

    Article  PubMed  CAS  Google Scholar 

  24. Van de Klundert, J. A. M., and Vliegenthart, J. S. (1993) PCR detection of genes coding for aminoglycoside-modifying enzymes, in Diagnostic Molecular MicrobioZogy-Principles and Applications, (Persing, D. H., Smith, T. F., Tenover, F. C., andWhite, T. J., eds.) ASM, Washington, D.C., 547–552.

    Google Scholar 

  25. Van Asselt, G. J., Vliegenthart, J. S., Petit, P. L. C., van de Klundert, J. A. M., and Mouton, R. P. (1992) High-level aminoglycoside resistance among enterococci and group A streptococci.J. Antimicrob. Chemother. 30 65l–659.

    Google Scholar 

  26. Petrin, J., Kuvelkar, R., Munayyer, H., Kettner, M., Hare, R. S., Miller, G. H., and Shaw, K. J. (1995) Cloning of two novel genes leading to aminoglycoside resistance. Abstr. Cold Spring Harbor Molecular Genetics of Bacteria and Phages #197.

    Google Scholar 

  27. Collis, C. M. and Hall, R. M. (1995) Expression of antibiotic resistance genes in the integrated cassettes of integrons. Antimicrob. Agents Chemother. 39, 155–162.

    PubMed  CAS  Google Scholar 

  28. Centron, C. and Roy, P. H. (1996) Analysis of a Serratia marcescens strain that harbors three integrons. Program Abst. Molecular Basis for Drug Resistance in Bacteria, Parasites and Fungi #403

    Google Scholar 

  29. Elisha, B. G., Segal, H., and Steyn, L. M. (1996) The promoter of a cryptic, integron associated ant(2“) gene from Acinetobacter baumannii is recognized in A. calcoaceticus. Program Abst. Molecular Basis for Drug Resistance in Bacteria, Parasites and Fungi #406

    Google Scholar 

  30. Shaw, K J., Rather, P. N., Sabatelli, F. J., Mann, P., Munayyer, H., Mierzwa, R., Petrikkos, G. L., Hare, R S., and Miller, G. H. (1992) Characterization of thechromosomal aac(6’)-Ic gene from Serratia marcescens. Antimicrob. AgentsChemother. 36, 1447–1455.

    CAS  Google Scholar 

  31. Rather, P. N., Orosz, E., Shaw, K. J., Hare, R., and Miller, G.(1993) Characterization and transcriptional regulation of the 2’-N-acetyltransferase gene from Providencia stuartii. J. Bacterial. 175, 6492–6498.

    CAS  Google Scholar 

  32. Tenover, F. C.(1988) Diagnostic deoxyribonucleic acid probes for infectious diseases. Clin. Microbial. Rev. 1, 82–101.

    CAS  Google Scholar 

  33. Schwocho, L. R., Schaffner, C., Miller, G. H., Hare, R. S., and Shaw, K. J. (1995) Cloning and characterization of a 3-N-aminoglycoside acetyltransferase gene, aac(3)-Ib, from Pseudomonas aeruginoasa. Antimicrob. Agents Chemother. 39, 1790–1796.

    CAS  Google Scholar 

  34. Levesque, C., Picht, L., Larose, C.,_and Roy, P. H.(1995) PCR mapping of.integrons reveals several novel combinations of resistance genes.Antimicrob.Agents Chemother. 39, 185–191.

    PubMed  CAS  Google Scholar 

  35. Miller, G. H., Sabatelli, F. J., Hare, R. S., Glupczynski, Y., Mackay, P., Shlaes, D., Shimizu, K., Shaw, K. J., and the Aminoglycoside Resistance Study Groups. (1997) The most frequent aminoglycoside resistance mechanism-changes with time and geographic area-a reflection of aminoglycoside usage patters? Clin. Infect. Dis. 24 suppl. l, 46–62.

    Google Scholar 

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Authors and Affiliations

  1. Schering-Plough Research Institute, Kenilworth, NJ

    Karen J. Shaw, Frank J. Sabatelli, Linda Naples, Paul Mann, Roberta S. Hare & George H. Miller

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  1. Karen J. Shaw
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  2. Frank J. Sabatelli
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  3. Linda Naples
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  4. Paul Mann
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  5. Roberta S. Hare
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Editors and Affiliations

  1. Central Public Health Laboratory, London, UK

    Neil Woodford  & Alan P. Johnson  & 

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© 1998 Humana Press Inc.

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Shaw, K.J., Sabatelli, F.J., Naples, L., Mann, P., Hare, R.S., Miller, G.H. (1998). The Application of Molecular Techniques for the Study of Aminoglycoside Resistance. In: Woodford, N., Johnson, A.P. (eds) Molecular Bacteriology. Methods in Molecular Medicine™, vol 15. Humana Press. https://doi.org/10.1385/0-89603-498-4:555

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  • DOI: https://doi.org/10.1385/0-89603-498-4:555

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-498-3

  • Online ISBN: 978-1-59259-599-0

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