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βLactam Resistance Mediated by Changes in Penicillin-Binding Proteins

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Molecular Bacteriology

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 15))

abstract

The widespread use, or perhaps overuse, of penicillin during the past 50 yr has driven the evolution of resistance to penicilling in numerous different species of bacteria.Typically, resistance has arisen as a result of the acquisition of β-lactamases that inactivate the antibiotic (see Chapter 25. Alternatively, in some Gram-negative bacteria, resistance may have arisen by a reduction in the ability of the antibiotic to access its target. However, in a number of clinically important Gram-negative and Gram-positive bacteria, resistance has arisen by alteration of the targets for penicillin and other β-lactam antibiotics, namely, the penicillin-binding proteins (PBPs).

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References

  1. Hansman, D.and Bullen, M.M. (1967) A resistant pneumococcus.Lancet II, 179–184

    Google Scholar 

  2. Klugman, KP(1990)Pneumococcal resistance to antibiotics.Clin Microbiol Rev 3, 171–96.

    Google Scholar 

  3. Dowson, C.G., Hutchison, A., Brantugan, J A., George, R.C., Hansman, D., Ltiares, J,T omasz, A, Smith, J.M., and Spratt, B.G (1989) Horizontal gene transfer of penicillin-bmding protein genes in penicillin-resistant clinical isolates of Streptococcus pneumonzae Proc Nat1 Acad Sci USA 86, 8842–8846

    Article  CAS  Google Scholar 

  4. Spratt, B G, Zhang, Q, Jones, D., Hutchison, A, Branmgan, J.A, and Dowson, C.G (1989) Recruitment of a pemcillm-binding protein gene from Nezsseria.flavescens during the emergence of penicillin resistance in Nesseria meningitidis Proc Natl.Acad Scr USA 86, 8988–8992

    Article  CAS  Google Scholar 

  5. Dowson, C G, Coffey, T.J, Kell, C, and Whiley, R.A (1993) Evolution ofpenicillin resistance in Streptococcus pneumoniae; the role of Streptococcusmitis in the formation of a low affinity PBP2B in S. pneumoniae Mol Microbiol 9, 635–643.

    Article  PubMed  CAS  Google Scholar 

  6. Martin, C, Bnese, T, and Hakenbeck, R.(1992) Nucleotide sequences of genes encoding peincillin-binding proteins from Streptococcus pneumomae and Streptococcus orahs with high homology to Escherichia coli penicillin-binding protein.1A and 1B.J Bacteriol 174, 4517–4523.

    PubMed  CAS  Google Scholar 

  7. Munoz, R, Coffey, T J., Daniels, M, Dowson, C.G., Laible, G, Casal, J, Hakenbeck R, Jacobs, M, Musser, J, Spratt, B G., and Tomasz, A(1991) Intercontinental spread of a multiresistant clone of serotype 23F Streptococcus.pneumoniae J Infect Du 164, 302–306

    CAS  Google Scholar 

  8. Coffey, T.J, Dowson, C.G, Damels, M., Zhou, J., Martin, C., Spratt, B G, and Musser, J.M.(1991) Horizontal transfer of multiple penicillin-binding protem genes, and capsular biosynthetic genes, in natural populations of Streptococcus pneumoniae Mol Microbiol 5, 2255–2260

    Article  PubMed  CAS  Google Scholar 

  9. Ward, J (1981) Antibiotic-resistant Streptococcus pneumoniae. clinical and epidemiological aspects.Rev Infect Dis 3, 254–266

    Article  PubMed  CAS  Google Scholar 

  10. Linares, J, Alonso, T, Perez, J.L., Ayats, J., Dommguez, M.A,. Pallares, R, and Martin, R(1992) Decreased susceptibility of penicillin-resistant pneumococci to twenty-four β-lactams antibiotics J Antimrcrob Chemother 30, 279–88

    Article  CAS  Google Scholar 

  11. Barcus, V A, Ghanekar, K, Yeo, M., Coffey, T.J., and Dowson, C.G(1995).Genetics of high-level penicillin resistance in clinical isolates of Streptococcus pneumonrae FEMS Microbiol Lett 126, 299–304

    Article  CAS  Google Scholar 

  12. Coffey, T J, Damels, M, McDougal, L K., Dowson, C G, Tenover, F C. and Spratt, B G.(1995) Genetics analysis of clmical isolates of Streptococcus pneumoniae wtth high-level resistance to expanded-spectrum cephalosporins Antimicrob Agents Chemother 39, 1306–1313

    PubMed  CAS  Google Scholar 

  13. Dowson C G., Johnson, A P., Cercenado, E., and George, R C(1994) Genetics of oxacillin resistance in clinical isolates of Streptococcus pneumoniae that are oxacillin resistant and peniciline susceptible Antimicrob Agents Chemother 38, 49–53

    PubMed  CAS  Google Scholar 

  14. Dougherty, T J., Koller, A.E., and Tomasz, A.(1980) penicillin binding proteins of peincillin-susceptible and intrinsically resistant Neisseria gonorrhoeae.Antlmlcrob Agents Chemother 18, 730–737.

    CAS  Google Scholar 

  15. Farukl, H and Sparling, P.F (1986) Genetics of resistance in a non-β-lactamase producing gonococcus with a relatively high level penicillin resistance Antimzcrob Agents Chemother 30, 856–860.

    Google Scholar 

  16. Mendelman, P.M., Caugant, D.A, Kalaitzoglou, G., Wedege, E., Chaffin, D O., Serfass, D A., Smith, A. L, Saez-Nieto, J.A, Vinas, M., and Selander, R K. (1989) Genetic diversity of penicillin G-resistant Neikseria meningitidis from Spain. Infect Immun 57, 1025–1029.

    PubMed  CAS  Google Scholar 

  17. Gulchard, D., Aubert, G., and Michel, V.P. (1994) What about penicllin reslstance.in N.eisserla menmgitidis? Med Ma1 Infect 24, 252–254

    Article  Google Scholar 

  18. National Committee for Clnical Laboratory Standards(1990) Performance standards for antimicrobial disk susceptibility tests. Approved standard M2-A4 NCCLS, Villanova PA

    Google Scholar 

  19. Report of the working party on antibiotic sensitivity testing of the British Society for Antlmlcrobia1 Chemotherapy(1991) A guide to sensltlvlty testing.J Antimicrob Chemother 27 Suppl. D

    Google Scholar 

  20. Pans, M M, Ramllo, O, and McCracken J, G.H(1995) Management of meningitis caused by penicillin-resistant Streptococcus pneumonlae Antimicrob Agents Chemother 39, 2171–2175.

    Google Scholar 

  21. Vlladrich, P.F., Gudlol, F., Ltiares, J, Rufi, G. Anza, J., and Pallares, R(1988) Characterlstlcs and antibiotic therapy of adult memngltls due to penicillin-reslstant pneumococcl Am J Med 84, 839–846.

    Article  Google Scholar 

  22. Jorgensen, J H., Ferraro, M.J McElmeel, M L, Spargo, J, Swenson, J.M,and Tenover, F.C.(1994) Detection of penicillin and extended-spectrum cephalosporm resistance among Streptococcus pneumonzae clinical isolates by use of theEtest J Clin Mcrobiol 32, 15–163.

    Google Scholar 

  23. Tenover, F.C, Baker, C.N, and Swenson, J.M. (1996) Evaluation of commercial methods for determining antimicrobial susceptibility of Streptococcus pneumomae.J Clan Mcrobiol 34, 10–14.

    CAS  Google Scholar 

  24. Skulnick, M, Small, G.W, Lo, P., Patel, M-P., Porter, C R., Low, D.E., Matsumura, S, and Mazzulli, T(1995) Evaluation of accuracy and reproducibility of E-test for susceptibility testing of Streptococcus pneumoniae to penicillin, cefotaxime, and ceftnaxone. J. Clin. Microbiol.33, 2334–2337

    PubMed  CAS  Google Scholar 

  25. Campos, J. Mendelman, P.M., Sako, M.V., Chaffin, D.0, Smith A L.,and Saez-Nieto, J.A(1987) Detection of relatively penicillin G-resistant Neisseria menlngltldis by disc susceptibility testing. Antimicrob Agents Chemother 31, 1478–1482.

    PubMed  CAS  Google Scholar 

  26. Campos, J Trujillo G., Seuba T, and Rodriguez A.(1992) Discriminative criteria for Neisseria meningitidis isolates that are moderately susceptible to penicillin and ampicillin.Antimicrob Agents Chemother.36, 1028–1031.

    PubMed  CAS  Google Scholar 

  27. Bardi, L. Badolati, A., Corso, A., and Rossi, M.A(1994) Failure of penicillin treatment of Nelsserla mennigitidis meningitis.Medicma-Buenos Awes 54, 427–430.

    CAS  Google Scholar 

  28. VanDyck, E, Smet, H., and Plot, P.(1994) Comparison of E-test with agar drlution for antimicrobial susceptibility testing of Neisseria gonorrhoeae J Clin Mwobiol 32, 1586–1588.

    Google Scholar 

  29. Gomezherruz, P Gonzalezpalacios, R., Romanyk, J, Cuadros, J.A, and Ena, J.(1995) Evaluatton of the E-test for penicillin susceptibility testing of Nelsserla meningitldls. Drag Microbiol Infect Dis. 21, 115–117

    Article  CAS  Google Scholar 

  30. Spratt, B.G.(1977) Properties of the penicillin-binding proteins of Escherlchia cobK12 Eur J Blochem 72, 341–352.

    Google Scholar 

  31. Gallem M, Lakaye, B Lepage, S, Jamin, M., Thamm, I., Jons, B., and Frere, J-M (1993) A new highly sensitive method for the detection and quantification of peincillin-binding proteins Biochem J 291, 19–21

    Google Scholar 

  32. Marktewicz, Z and Tomasz, A(1989) Variation in penicillin-binding protein patterns of penicillin-resistant cluucal isolates of pneumococci J Clin Microbiol 27, 405–410

    Google Scholar 

  33. Jabes, D. Nachman, S., and Tomasz, A. (1989) penicillin-binding protein famrlies: evidence for the clonal nature of penicillin resistance in clinical isolates of pneumococct. J Infect Dis 159, 16–25.

    PubMed  CAS  Google Scholar 

  34. Hakenbeck, R., Bnese T, Ellerbrok, H., Laible, G, Martin, C, Metelmann, C., Schrer, H-M, and Tornette, S(1988) Targets of β-lactams, in Streptococcus pneumoniae, in Antrbiotic Inhlbition of Cell Surface Assembly and Function, (Actor, P, Daneo-Moore, L, Higgins, M L., Salton, M. R J, and Shockman, G. D., eds), American Society for Microbrology, Washington,D C, pp 390–399

    Google Scholar 

  35. Zhang, Q-Y., Jones D M., Saez Nieto, J.A., Perez Trallero, E, and Spratt, B. G (1990) Genetic diversity of penicillin-binding protein 2 genes of pemcillmresistant strains of Newseria menmgitidis revealed by tingerprinting of amplified DNA Antimicrob Agents Chemother 34, 1523–1528

    PubMed  CAS  Google Scholar 

  36. Kell, C. M. Jordens, J.Z., Damels, M., Coffey, T. J., Bates J. Paul, J, Grlks, C,and Spratt, B.G. (1993) Molecular epidemiology of pemcrllm-reststant pneumococcr isolated in Nairobi, Kenya Infect. Immun.61, 4382–4391.

    PubMed  CAS  Google Scholar 

  37. Klugman, K.P. Coffey, T.J, Smith, A., Wasas, A., Meyers, M, and Spratt, B G (1994) Cluster of an erythromycin-resistant variant of the Spanish multiply-resistant 23F clone of Streptococcus pneumoniae in South Africa Euro J Clan Microbiol Infect Dis 13, 171–174

    Article  CAS  Google Scholar 

  38. Smrth, A.M and Klugman, K P.(1995) Alterations in penicillin-binding protein 2B from penicillin-resistant wild-type strains of Streptococcus pneumonae Antimicrob Agents Chemother. 39, 859–867

    Google Scholar 

  39. Selander, R.K., Caugant D.A., and Whittam T.S. (1987) Genetic structure and variation in natural populations of Escherichla cob, in Cellular and Molecular Biology vol 2., (Nerdhardt, F. C., ed), American Society of Microbiology, Washington D.C., pp. 1625–1648.

    Google Scholar 

  40. Selander, R.K. and Smith, N.H(1990) Molecular population genetics of Salmonella Med Rev. Microbiol 1, 219–228

    Google Scholar 

  41. Spratt, B. G, Smith, N.H. Zhou, J, O’Rourke, M., and Feil, E.(1995) The population genetics of the pathogenic Neisseaia, in The Population Genetics of Bactena, (Baumberg, S., Young, J. P. W., Wellington, E. M. H., and Saunders, J R., eds.), SGM Symposium No 52, Cambridge University Press, Cambridge,pp. 143–160

    Google Scholar 

  42. Hall, L. M. C., Whrley, R. A, Duke, B. George, R. C., and efstratiou, A.(1996) Genetic relatedness within and between serotypes of Streptococcus pneumoniae from the United-Kingdom-analysts of multilocus enzyme electrophoresis, pulsed-field gel electrophoresis, and antimcrobial resistance patterns.J Clin Muiroblol 34, 853–859

    CAS  Google Scholar 

  43. Selander, R.K. and Levm, B. R (1980) Genetic diversity and structure in Escherichia coli populations Science 210, 545–547

    Article  PubMed  CAS  Google Scholar 

  44. Caugant, D A, Froholm, L. O, Bovre, K., Holten, E, Frasch, C.E, Mecca, L F., Zollinger, W. D and Selander, R K. (1987) Intercontinental spread of Nelsseria meningltidis clones of the ET-5 complex Antome van Leeuwenhoek J Microbiol 53, 389–394

    CAS  Google Scholar 

  45. Dowson, C G. Coffey, T J., and Spratt, B.G.(1994) origm and molecular epidemiology of penicillin-binding-protein-mediated resistance to S-lactam antibtotics Trends Microblol 2, 361–366

    Article  CAS  Google Scholar 

  46. Ubukata, K., Asahi, Y., Yamane A, and Konno, M(1996) Combinational detection of autolysin and penicillin-binding protein 2B genes of Streptococcus pneumonlae by PCR J Clin Microbiol. 34, 592–596

    PubMed  CAS  Google Scholar 

  47. Dowson, C G. Hutcitson, A, and Spratt, B.G. (1989) Nucleotide sequence of the penicillin-binding protein 2B gene of Streptococcus pneumonlae strain R6. Nucleic Acid Res. 17, 7518

    Article  PubMed  CAS  Google Scholar 

  48. Lalble, G., Spratt, BG, and Hakenbeck, R (1991) Interspecies recombinational events during the evolution of altered PBP 2X genes m penicillin-resistant clincal isolates of Streptococcus pneumoniae. Mel Microbiol. 5, 1993–2002.

    Article  Google Scholar 

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Authors and Affiliations

  1. Biological Sciences, University of Warwick, Coventry, UK

    Christopher G. Dowson & Tracey J. Coffey

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  1. Christopher G. Dowson
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  2. Tracey J. Coffey
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  1. Central Public Health Laboratory, London, UK

    Neil Woodford  & Alan P. Johnson  & 

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© 1998 Humana Press Inc.

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Dowson, C.G., Coffey, T.J. (1998). βLactam Resistance Mediated by Changes in Penicillin-Binding Proteins. In: Woodford, N., Johnson, A.P. (eds) Molecular Bacteriology. Methods in Molecular Medicine™, vol 15. Humana Press. https://doi.org/10.1385/0-89603-498-4:537

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  • DOI: https://doi.org/10.1385/0-89603-498-4:537

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-498-3

  • Online ISBN: 978-1-59259-599-0

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