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Campylobacter Infections

Species Identification and Typing

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Molecular Bacteriology

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 15))

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Abstract

Campylobacters are the most frequently identified cause of acute bacterial diarrhea in humans in England and in many other developed countries. Although C jejuni and C coli are numerically the most important species in cases of campylobacter enteritis, there is a growing awareness that some of the other 13 species of Campylobactermay also be linked with human disease. However, precise identification of these organisms is rarely carried out in clincal laboratories, and the significance of most species or subtypes in causing human disease is unknown

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References

  1. Blaser, M. J., Hardesty, H. L, Powers, B., and Wang, W. L L (1980) Survival of Campylobacter fetus subsp jejuni in biological miheus.J Clin Microbiol 11, 309–313

    PubMed  CAS  Google Scholar 

  2. Robinson, D A (1981) Infecttve dose of Campylobacter jejuni in milk B M J 282, 1584

    Article  CAS  Google Scholar 

  3. Healing, T D., Greenwood, M H., and Pearson, A D. (1992) Campylobacter and enteritis Rev. Med Microbiol 3, 159–167.

    Google Scholar 

  4. Totten, P. A, Patton, C M., Tenover, F C, Barrett, T J, Stamm, W E, Steigerwalt, A G., Lin, J. Y, Holmes, K K., and Brenner, D. J. (1987) Prevalence lence and characterization of hippurate-negative Campylobacter jejuniin King Country, Washington J Clin Microbiol 25, 1747–1752.

    CAS  Google Scholar 

  5. Owen, R J (1995) Recent developments in the molecular systematics of campylobacters and arcobacters in Molecular Approaches to Food Safety, (Eklund, M., Richard, J. L., and Mise, K., eds), Alaken, Fort Collins, CO, pp 19–38

    Google Scholar 

  6. Eyers, M, Chapelle, S., Van Camp, G., Goossens, H, and De Wachter, R (1993)Discrimination among thermophilic Campylobacter species by polymerase chain reaction amplification of 23S rRNA gene fragments J Clin Microbiol 31, 3340–3343.

    PubMed  CAS  Google Scholar 

  7. Bastyns, K, Chapelle, S., Vandamme, P, Goossens, H, and De Wachter, R. (1994)Species-specific detection of campylobacters important in veterinary medicine by PCR amplification of 23S rDNA areas System Appl Microbiol 17, 563–568.

    Google Scholar 

  8. Cardareli-Leite, P, Blom, K, Patton, C. M, Nicholson, M A., Steigerwalt, A. G, Hunter, S. B, Brenner, D J, Barrett, T. J, and Swaminathan, B. (1996) Rapid identification of Campylobacter species by restriction fragment length polymorphism analysis of a PCR-amplified fragment of the gene encodmg for 16S rRNA J Clin Microbiol 34, 62–67

    Google Scholar 

  9. Giesendorf, B. A J, Van Belkum, A, Koeken, A, Stegeman, H, Henkens, M H C, van der Plas, J, Gossens, H., Niesters, H G M, and Quint, W G V (1993) Development of species-specific DNA probes for Campylobacter jejuni, Campylobacter coli, and Campylobacter lari by polymerase chain reaction fingerprinting J Clin Microbiol 31, 1541–1546.

    PubMed  CAS  Google Scholar 

  10. Owen, R J and Gibson, J. R (1995) Update on epidemiological typing of Campylobacter.PHLS Microbiol Digest 12, 2–6.

    Google Scholar 

  11. Lior, H (1984)New extended biotyping scheme for Campylobacter jejuni, Campylobacter coli, and “Campylobacter laridis ” J Clin Mlcrobiol 20, 636–640.

    CAS  Google Scholar 

  12. Bolton, F. J., Wareing, D. R A, Skirrow, M B, and Hutchinson, D N (1992)Identification and biotyping of campylobacters in Identication Methods in Applied and Environmental Microbiology, (Board, R G, Jones, D., and Skinner, F A, eds.), SAB Technical Series 29 Academic Press, London, pp 151–161

    Google Scholar 

  13. Owen, R. J, Lorenz, E, and Gibson, J (1997) Application of the MAST resistotyping scheme to Campylobacter jejuni and Campylobacter co1i J Med Microbiol (bd46, 34–38

    Article  Google Scholar 

  14. Penner, J. L. and Hennessy, J N. (1980) Passive hemagglutination technique for serotyping Campylobacter fetus subsp jejuni on the basis of soluble heat-stable antigenic J Clin Microbiol, 12, 732–737

    PubMed  CAS  Google Scholar 

  15. Lior, H., Woodward, D. L., Edgar, J. A., Laroche, L J, and Gill, P (1982) Serotyping of Campylobacter jejuni by slide agglutmation based on heat-labile antigenic factors. J Clin Microbiol 15, 761–768

    PubMed  CAS  Google Scholar 

  16. Khakhria, R and Lior, H. (1992) Extended phage-typing scheme for Campylobacter jejuni and Campylobacter coli. Epidem Infect 108, 403–414.

    Article  CAS  Google Scholar 

  17. Salama, S M, Bolton, F J., and Hutchinson, D N (1990) Application of a new phage typing scheme to campylobacters isolated during outbreaks J. Epidemiol Infect 104, 405–411.

    Article  CAS  Google Scholar 

  18. Bradbury, W C, Pearson, A. D, Marko, M. A., Congi, R. V., and Penner, J. L (1984) Investigation of a Campylobacter jejuni outbreak of serotyping and chromosomal restriction endonuclease analysis. J Clin Microbiol 19, 342–346

    PubMed  CAS  Google Scholar 

  19. Owen, R. J., Costas, M, and Dawson C (1989) Application of different chromosomal DNA fingerprints to specific and subspecific identification of Campylobatter isolates J Clin Microbiol 27, 2338–2343

    PubMed  CAS  Google Scholar 

  20. Owen, R J and Hernandez, J (1990) Genotypic variation in “Campylobacter upsaliensis” from blood and faeces of patients in different countries FEMS Microbiol Lett 72, 5–10

    Article  CAS  Google Scholar 

  21. Lind L. SJogren E Melby K. and Kaijser B 1996 DNA fingerprinting and serotyping of Campylobacter jejuni isolates from epidemic outbreaks. J Clin Microbiol 34 892–896

    Google Scholar 

  22. Yan, W., Chang, W, and Taylor, D E. (1991) Pulsed-field gel electrophoresis of Campylobacter jejuni and Campylobacter coli genomic DNA and its epidemiological application. J Infect Dis 163, 1068–1072

    PubMed  CAS  Google Scholar 

  23. Owen, R J., Sutherland, K, Fitzgerald, C., Gibson, J., Borman, P., and Stanley, J (1995) Molecular subtyping scheme for serotypes HS1 and HS4 of Campylobacter jejuni.J Clin. Patho1 33, 872–877.

    CAS  Google Scholar 

  24. Stanley, J. D., Linton, D, Sutherland, K., Jones, C., and Owen, R. J (1995)High-resolution genotyping of Campylobacter coli identifies clones of epidemiologic and evolutionary significance J. infect Dis 172, 1130–1134

    PubMed  CAS  Google Scholar 

  25. Gibson, J. R, Fitzgerald, C., and Owen, R J (1995)Comparison of PFGE, ribotyping and phage typing in the epidemiological analysis of Campylobacter jejuni serotype HS2 infections Epidem Infect 115, 215–225

    Article  CAS  Google Scholar 

  26. Gibson, J R, Sutherland, K., and Owen, R. J. (1994) Inhibition of DNase activity in PFGE analysis of DNA from Campylobacter jejuni Lett Appl Microbiol. 19, 357–358

    Article  PubMed  CAS  Google Scholar 

  27. Fayos, A, Owen, R. J., Desai, M., and Hernandez, J (1992)Ribosomal RNA gene restriction fragment diversity amongst Lior biotypes and Penner serotypes of Campylobacter jejuni and Campylobacter coli.FEMS Microbiol Lett 95, 87–94.

    Article  CAS  Google Scholar 

  28. Patton, C. M, Wachsmuth, I. K, Evins, G. M, Kiehlbauch, J A., Plikaytis, B. D, Troup, N., Tompkins, L., and Lior, H. (1991) Evaluation of 10 methods to distinguish epidemic associated Campylobacter strains.J Clin Microbiol 29, pp680–688.

    Google Scholar 

  29. Owen, R. J, Desar, M., and Garcia, S (1993) Molecular typing of thermotolerant species of Campylobacter with ribosomal RNA gene patterns.Res Microbiol 144, 709–720

    Article  PubMed  CAS  Google Scholar 

  30. Fitzgerald, C., Owen, R. J., and Stanley, J. (1996)Comprehensive ribotyping scheme for heat-stable serotypes of Campylobacter jejuni.J Clin Microbiol 34, 265–269.

    PubMed  CAS  Google Scholar 

  31. Owen, R. J., Fitzgerald, C., Sutherland, K., and Borman, P. (1994)Flagellin gene polymorphism analysis of Campylobacter jejuni infecting man and other hosts and comparison with biotyping and somatic antigen serotyping.Epidemiol Infec 113, 22l–234

    Google Scholar 

  32. Nachamkin, I., Bohachick, K., and Patton, C M. (1993) Flagellin gene typing of Campylobacter jejuni by restriction fragment length polymorphism analysis.J Clin Microbiol 31, 1531–1536

    PubMed  CAS  Google Scholar 

  33. Birkenhead, D, Hawkey, B M., Heritage, J, Gascoyne-Binzi, D M, and Kite, P (1993)PCR for the detection and typing of campylobacters Lett Appl Microbiol 17, 235–237

    Article  PubMed  CAS  Google Scholar 

  34. Alm, R A, Guerry, P, and Trust, T. J (1993) Distribution and polymorphism of the flagellin genes from Isolates of Campylobacter coli and Campylobacter jejuni J Bacteriol 175, 3051–3057

    PubMed  CAS  Google Scholar 

  35. Burnens, A. P, Wagner, J, Lior, H., Nicolet, J, and Frey, J (1995) Restriction fragment length polymorphisms among the flagellar genes of the Lior heat-labile serogroup reference strains and field strains of Campylobacter jejuni and C co1i Epidemiol Infect 114, 423–431

    Article  PubMed  CAS  Google Scholar 

  36. Mazurier, S, Van de Guessen, A, Heuvelman, K, and Wernars, K (1992)RAPD analysis of Campylobacter isolates, fingerprinting without the need to purify DNA Lett Appl Microbiol 14, 260–262

    Article  PubMed  CAS  Google Scholar 

  37. Owen, R J and Hernandez, J. (1993)Ribotyping and arbitrary-primer PCR fingerprinting of campylobacters, in New Techniques in Food and Beverage Microbiology,(Kroll, R G, Gilmour, A, and Sussman, M., eds.), SAB Tech Ser 31, Blackwell, Oxford, pp265–285

    Google Scholar 

  38. Fayos, A, Owen, R. J., Hernandez, J., Jones, C., and Lastovica, A (1993)Molecular subtyping by genome and plasmid analysis of Campylobacter jejuni serogroups 01 and 02 (Penner) from sporadic and outbreak cases of human diarrhoea.Epidemiol Infect 111, 415–427

    Article  PubMed  CAS  Google Scholar 

  39. Giesendorf, B. A J., Goossens, H, Niesters, H G. M, van Belkum, A., Koeken, A., Enditz, H P, Stegeman, H, and Quint. W G V (1994)Polymerase chain reaction-mediated DNA fingerprinting for epidemtological studies on Campylobatter spp.J. Med Microbiol.40, 141–147

    Article  PubMed  CAS  Google Scholar 

  40. Iriarte, M. and Owen, R J (1996)Repetitive and arbitary primer DNA sequences in PCR-medicated fingerprinting of outbreak and sporadic isolates of Campylobacter jejuni FEMS Immunol Med Microbiol 648, 17–22

    Article  Google Scholar 

  41. Lind, L., Sjogren, E, Welinder-Olsson, C, and Kaijser, B (1989) Plasmids and serogroups in Campylobacter jejuni APMIS 97, 1097–I102.

    Article  PubMed  CAS  Google Scholar 

  42. Owen, R. J and Hernandez, J (1990)Occurrence of plasmids in “Campylobacter upsaliensis” (catalase negative or weak group) from geographically diverse patients with gastroenteritis or bacteraemia Eur J Epidemiol 6, 11l–117.

    Article  Google Scholar 

  43. Stanley, J., Jones, C, Burnens, A., and Owen, R J (1994)Distinct genotypes of human and canine isolates of Campylobacter upsaliensis determined by 16S rRNA gene typing and plasmid profiling J Clin Microbiol.32, 1788–1794

    PubMed  CAS  Google Scholar 

  44. Oyofo, B A., Thornton, S A, Burr, D H., Trust, T J., Pavloskis, 0. R, and Guerry, P (1992)Specific detection of Campylobacter jejuni and Campylobacter coli by using polymerase chain reaction J Clin Microbiol 30, 26313–2619

    Google Scholar 

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Author information

Authors and Affiliations

  1. Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, UK

    Janet R. Gibson

  2. Laboratory of Enteric Pathogensity of Texas Medical School, Houston, TX

    Robert J. Owen

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  1. Janet R. Gibson
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  2. Robert J. Owen
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Editors and Affiliations

  1. Central Public Health Laboratory, London, UK

    Neil Woodford  & Alan P. Johnson  & 

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© 1998 Humana Press Inc.

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Gibson, J.R., Owen, R.J. (1998). Campylobacter Infections. In: Woodford, N., Johnson, A.P. (eds) Molecular Bacteriology. Methods in Molecular Medicine™, vol 15. Humana Press. https://doi.org/10.1385/0-89603-498-4:407

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  • DOI: https://doi.org/10.1385/0-89603-498-4:407

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-498-3

  • Online ISBN: 978-1-59259-599-0

  • eBook Packages: Springer Protocols

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