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Molecular Methods for Haemophilus influenzae

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Molecular Bacteriology

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 15))

Abstract

The speciesHaemophilus influenzae belongs to the genus Haemophilus and the family Pasteurellaceae. H influenzae are small, nonmotile, nonspore forming, Gram-negative, pleomorphic rods that range in shape from coccobacilli to long filaments. They require X and V factors (hemin and NAD, respectively) for aerobic growth, and may be facultatively anaerobic (1) Encapsulated H. influenzae are classified into six antigenically distinct serotypes (a–f), and have a clonal population structure with two major global subdivisions (I and II) (2,3). Nonencapsulated H. infuenzae (NCHi) appear to have a nonclonal population composition, but a broader analysis of NCHi in the future may reveal that the population is not so distinct from encapsulated H influenzae (4). Both encapsulated H influenzae and NCHi exhibit wide genetic diversity (5).

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Authors and Affiliations

  1. Paediatric Infectious Diseases Group, Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK

    Mark A. Herbert & E. Richard Moxon

  2. Oxford Public Health Laboratory, Oxford, UK

    Derrick Crook

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  1. Central Public Health Laboratory, London, UK

    Neil Woodford  & Alan P. Johnson  & 

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Herbert, M.A., Crook, D., Moxon, E.R. (1998). Molecular Methods for Haemophilus influenzae. In: Woodford, N., Johnson, A.P. (eds) Molecular Bacteriology. Methods in Molecular Medicine™, vol 15. Humana Press. https://doi.org/10.1385/0-89603-498-4:243

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  • DOI: https://doi.org/10.1385/0-89603-498-4:243

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