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Molecular Approaches to the Identification of Streptococci

  • Robert E. McLaughlin
  • Joseph J. Ferretti
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 15)

Abstract

The ability to identify rapidly organisms to the species, and at times subspecies level, is an important step in the treatment of bacterial infections and for monitoring the spread of microorganisms. Conventional identification of streptococci relies on the isolation and culturing of bacterial cells, and then submitting the culture to a battery of biochemical tests. Whereas these panels are useful and have a fairly high degree of accuracy, they can suffer from preparation time and problems with the identification of nutritionally variant strains (mainly with the viridans streptococci). Biochemical classification also lacks the ability to type species to the level of a particular clonal population or strain. Although not as important from the diagnostic perspective, the ability to type bacteria to the clonal level is important for epidemiologic studies of disease outbreaks.

Keywords

Polymerase Chain Reaction Product Brain Heart Infusion Microfuge Tube Restriction Fragment Length Polymorphis Late Logphase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 1998

Authors and Affiliations

  • Robert E. McLaughlin
    • 1
  • Joseph J. Ferretti
    • 1
  1. 1.Department of Microbiology and ImmunologyUniversity of Oklahoma Health Science CenterOklahoma City

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