Advertisement

Chancroid

  • Stephen A. Morse
  • David L. Trees
  • Patricia A. Totten
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 15)

Abstract

Chancroid is a genital ulcerative disease GUD). These diseases are common throughout the world and include syphilis, genital herpes, chancroid, lymphogranuloma venereum, and donovanosis. Chancroid is particularly common an Africa, Asia, and Latin America where its incidence may exceed that of syphilis as a cause of genital ulceration (1,2). However, chancroid is considered an uncommon sexually transmitted infection in the United States

Keywords

Polymerase Chain Reaction Sodium Dodecyl Sulfate Polymerase Chain Reaction Assay Genital Herpes Genital Ulcer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Orttz-Zepeda, C, Hernandez-Perez, E., and Marroquuin-Burgos, R (1994) Gross and mrcroscoprc features in chancroid: a study in 200 new culture-proven cases in San Salvador. Sex Transm Dis 21, 112–117.CrossRefGoogle Scholar
  2. 2.
    Piot, P and Plummer, F. A. (1990) Genital ulcer adenopaty syndrome, in Sexually Transmitted Diseases. 2nd ed. (Holmes, K. K., Mardh, P-A., Sparlmg, P. F., Weisner, P. J., Cates, Jr., W., Lemon, S. M., and Stamm, W. E., eds.), McGraw-Hill, New York, pp. 711–716Google Scholar
  3. 3.
    Casin, I, Grimont, F, Grimont, P. A. D., and Sanson-Le Pors, M.-J (1985) Lack of deoxyribonucleic acid relatedness between Haemophilus ducreyi and other Haemophilus species Int J Syst. Bacterial 35, 23–25.CrossRefGoogle Scholar
  4. 4.
    Rossau, R., Duhamel, M, Jannes, G, Decourt, J L, and Van Heuverswyn, H (1991) The development of specific r-RNA-denved ohgonucleottde probes for Haemophdus ducreyi, the causative agent of chancroid. J. Gen Muzroblol 137, 277–285Google Scholar
  5. 5.
    Dewhtrst, F. E, Paster, B. J, Olsen, I, and Fraser, G. J (1992) Phylogeny of 54 representative strains of species in the family Pasteurellaceae as determined by compartson of 16SrRNA sequences J Bacterzol 174, 2002–2013Google Scholar
  6. 6.
    Parsons, L M, Shayegam, M, Waring, A L, and Bopp, L H (1989) DNA probes for the tdenttfication of Haemophllus ducreyi J Clin Mlcrobiol 27, 1441–1445Google Scholar
  7. 7.
    Parsons, L M, Shayegam, M, Waring, A L, and Bopp, L. H (1990) Construction of DNA probes for the tdentification of Haemophllus ducreyz in Gene Probes for Bacteria (Macario, A J., and Conwayde Macario, E, eds), Academtc, New York, pp. 69–94Google Scholar
  8. 8.
    Parsons, L M, Warmg, A. L, and Shayegam, M. (1992) Molecular analysts of theHaemophllus ducreyi groE heat shock operon Infect Immun 60, 4111–4118PubMedGoogle Scholar
  9. 9.
    Chui, L, Albrttton, W, Paster, B., Maclean, I, and Marusyk, R (1993) Development of the polymerase cham reaction for dtagnosts of chancrotd J Clin Mzcroblol 31, 659–664.Google Scholar
  10. 10.
    Johnson, S. R, Martin, D H., Cammarata, C, and Morse, S. A (1994) Development of a polymerase chain reactton assay for the detection of Haemophllus ducreyl Sex Transm DIS 21, 13–23PubMedCrossRefGoogle Scholar
  11. 11.
    Johnson, S R, Martm, D. H, Cammarata, C, and Morse, S A (1995) Diagnosis of chancrotd by a polymerase chain reactton assay alterations in sample preparation increase sensitivity. J Clm Mlcroblol 33, 1036–1038Google Scholar
  12. 12.
    Orle, K A, Gates, C A, Martin, D H., Body, B. A, and Weiss, J B. (1996) Smultaneous PCR detection of Haemophdus ducreyz, Treponema pallidurn, and Herpes Simplex Viruses Types-1 and-2 from genital ulcers J Clm Mzcrobzol 34, 49–54Google Scholar
  13. 13.
    Odumeru, J A, Ronald, A. R, and Albrttton, W L(1983) Charactertztion of cell proteins of Haemophzlus ducreyi by polyacrylamtde gel electrophorests J Infect Dis 148, 710–714PubMedGoogle Scholar
  14. 14.
    Taylor, D N., Escheverria, P, Hanchalay, S, Pttarangst, C, Slootmans, L, and Plot, P. (1985) Anttmlcrobtal suscpettbtlity and charactertzation of outer membrane proteins of Haemophllus ducreyi Isolated in Thailand J Clrn Mwrobiol 21, 442–444.Google Scholar
  15. 15.
    Slootmans, L., Vanden Berghe, D A, and Plot, P (1985) Typing Haemophzlus ducreyi by indirect immunofluorescence assay Genltourln Med 61, 123–126.Google Scholar
  16. 16.
    Van Dyck, E and Plot, P. (1987) Enzyme profiles of Haemophllus ducreyi strains isolated on drfferent continents. Eur. J. Clin Mlcroblol 6, 40–43.CrossRefGoogle Scholar
  17. 17.
    Kortmg, H C, Abeck, D, Johnson, A P, Ballard, R C., Taylor-Robinson, D, and Braun-Falco, O. (1988) Lectin typing of Haemophzlus ducreyi Eur J Clin Microbiol Infect Dis 7, 676–680Google Scholar
  18. 18.
    Schalla, W O. and Morse, S A. (1994) Epidemtologrcal applications of lectins to agents of sexually transmitted diseases in Lectw-Mzcroorganism Interactzons (Doyle, R. J and Sltfkm, M., eds), Marcel Dekker, New York, pp. 111–142Google Scholar
  19. 19.
    Irino, K, Grimont, F, Casin, I., Grimont, P A D., and The Brazilian Purpuric#@#Fever Study Group. (1988) rRNA gene restriction patterns of Huemophzlus influenzae biogroup aegyptius associated with Brazilian purpuric fever. J Clin Mzcrobzol 26, 1535–1538.Google Scholar
  20. 20.
    Yogev, D., Halachmt, D., Kenny, G. E, and Razin, S (1988) Distinction of species and strains of mycoplasmas (molhcutes) by genomic DNA fingerprmts with an rRNA gene probe J Clzn Mzcrobzol 26, 1198–1201.Google Scholar
  21. 21.
    Sarafian, S. K., Woods, T C, Knapp, J S, Swaminathan, B., and Morse, S A (1991)Molecular characterrzation of Haemophzlus ducreyi by rtbosomal DNA fingerprinting J Clzn Mzcrobzol 29, 1949–1954Google Scholar
  22. 22.
    Brown, T. J and Ison, C A (1993) Non-radioactive ribotyping of Haemophilus ducreyi using a digoxrgenin labelled cDNA probe Epidemiol Infect 110, 289–295.PubMedCrossRefGoogle Scholar
  23. 23.
    Ballard, R. and Morse, S A. (1996) Chancroid in Atlas of Sexually Transmitted Diseases and AIDS, 2nd ed (Morse, S. A., Moreland, A, and Holmes, K K, eds.), Mosby-Wolfe, London, pp 47–63Google Scholar
  24. 24.
    Flood, J M., Saratian, S K., Bolan, G A, Lammel, C., Engelman, J, Greenblatt, R M., Brooks, G F, Back, A., and Morse, S. A (1992) Multistrain outbreak of chancroid in San Francisco, 1989-1991 J Infect Dis 167, 1106–1111Google Scholar
  25. 25.
    Melaugh, W., Phillips, N J., Campagnan, A A, Karalus, R, and Gibson, B W. (1992) Partial characterization of the maJor lipooligosaccharide from a strain of Haemophzlus ducreyz, the causative agent of chancroid, a genital ulcer disease J Biol Chem 267, 13,434–13,439.PubMedGoogle Scholar
  26. 26.
    Odumeru, J. A, Wiseman, G. M., and Ronald, A. R. (1985) Role of bpopolysaccharade and complement in susceptibility of Haemophzlus ducreyi to human serum Infect Immun 50, 495–499.PubMedGoogle Scholar
  27. 27.
    Odumeru, J A, Wiseman, G M., and Ronald, A. R (1987) Relationship between lipopolysaccharide composition and vnulence of Haemophzlus ducreyz J Med Mzcrobiol 23, 155–162CrossRefGoogle Scholar
  28. 28.
    Alfa, M J (1992) Cytopathic effect of Haemophzlus ducreyi for human foreskin cell culture J Med Mzcrobzol 37, 43–50CrossRefGoogle Scholar
  29. 29.
    Totten, P. A, Norn, D V, and Stamm, W E (1995) Characterization of the hemolytic actrvity of Haemophzlus ducreyi Inf Immun 63, 4409–4416Google Scholar
  30. 30.
    BrentJens, R. J, Ketterer, M., Apicella, M. A., and Spmola, S M (1996) Fine tangled pill expressed by Haemophrlus ducreyi are a novel class of pili. J Bacteriol 178, 808–816.PubMedGoogle Scholar
  31. 31.
    Elkins, C. (1995) Identificatton and purification of a conserved heme-regulated hemoglobm-binding outer membrane protein from Haemophzlus ducreyi Infect Immun 63, 1241–1245PubMedGoogle Scholar
  32. 32.
    Elkms, C., Chen, C., and Thomas, C. E. (1995) Charactertzation of the HgbA locus encoding a hemoglobin receptor from Haemophzlus ducreyi Infect Immun 2194–2200.Google Scholar
  33. 33.
    Lammel, C J., Dekker, N P, Palefsky, J., and Brooks, G F. (1993) In-vitro model of Haemophzlus ducreyi adherence to and entry into eukaryotic cells of genital origin. J Infect Dis. 167, 642–650PubMedGoogle Scholar
  34. 34.
    Totten, P A., Lara, J C, Norm, D V, and Stamm, W. E. (1994) Haemophzlus ducreyi attaches to and invades cultured human foreskin epithelial cells in vitro Infect Immun 62, 5632–5640PubMedGoogle Scholar
  35. 35.
    Willson, P J, Albritton, W L., Slaney, L., and Setlow, J. K (1989) Characterization of a multiple antibiotic resistance plasmid from Haemophilus ducreyi Antimicrob Agents Chemother 33, 1627–1630PubMedGoogle Scholar
  36. 36.
    Hansen, E. J, Latimer, J L, Thomas, S E., Helminen, M., Albritton, W L, and Radolf, J. D (1992) Use of electroporatton to construct lsogemc mutants of Haemophilus ducreyi. J Bacterzol. 174, 5442–5449.Google Scholar
  37. 37.
    Palmer, K. L and Munson, J R. S (1995) Cloning and characterization of the genes encoding the haemolysin of Haemophzlus ducreyz Mol Mzcrobzol 18, 821–830CrossRefGoogle Scholar
  38. 38.
    Stevens, M K., Cope, L. D., Radolf, J. D., and Hansen, E J. (1995) A system for generalized mutagenesis of Haemophzlus ducreyi Infect Immun 63, 2976–2982PubMedGoogle Scholar
  39. 39.
    Alfa, M J, Degagne, P., and Hollyer, T (1993) Haemophzlus ducreyi adheres to but does not invade cultured human foreskin cells Infect Immun 61, 1735–1742PubMedGoogle Scholar
  40. 40.
    Palmer, K L, Grass, S, and Munson, R. S, Jr. (1994) Identification of a hemolytic activity elaborated by Haemophilus ducreyi Infect Immun 62, 3041–3043PubMedGoogle Scholar
  41. 41.
    Hollyer, T T., DeGagne, P A., and Alfa, M. J (1994) Characterization of the cytopathic effect ofHaemophilus ducreyi Sex Trans Dis 21, 247–257.CrossRefGoogle Scholar
  42. 42.
    Purven, M and Lagergard, T (1992) Haemophilus ducreyi, a cytotoxin-producing bacterium Infect Immun 60, 1156–1162.PubMedGoogle Scholar
  43. 43.
    Alfa, M. J, DeGagne, P., and Totten, P. A (1996) Haemophilus ducreyi hemolysin acts as a contact-cytotoxin and damages human foreskin fibroblasts in cell culture Infect Immun 43, 2349–2352Google Scholar
  44. 43a.
    Palmer, K L, Goldman, W E, and Munson, R S Jr (1996) An isogenic haemolysin-deficient mutant of Haemophilus ducreyi lacks the ability to produce cytopathic effects on human foreskin fibroblasts Mol Mzcrobiol 21, 13–19CrossRefGoogle Scholar
  45. 44.
    Purcell, B K, Richardson, J A, Radolf, J D., and Hansen, E J (1991) A temperature-dependent rabbit model for production of dermal lesions by Haemophilus ducreyi J Infect Dis 164, 359–367PubMedGoogle Scholar
  46. 44a.
    Hobbs, M M., San Mateo, L R., Omdorff, P E, Almond, G., and Kawula, T H. (1995) Swine model of Haemophilus ducreyi mfection Infect Immun 63, 3094–3100PubMedGoogle Scholar
  47. 44b.
    Spmola, S. M., Weld, L M., Apicella, M A, Gaspari, A. A, and Campagnari, A A (1994) Experimental human mfection with Haemophilus ducreyi.J Infect. Dis 169, 1146–1150.Google Scholar
  48. 44c.
    Totten, P. A, Morton, W. R., Knitter, G. H, Clark, A M., Iovtat, N. B., and Stamm, W. E. (1994) A primate model for chancrod J Infect Dis 169, 1284–1290.PubMedGoogle Scholar
  49. 45.
    Spmola, S M., Griffiths, G. E, Shanks, K. L., and Blake, M S. (1993) The maJor outer membrane protein ofHaemophilus ducreyi is a member of the OmpA family of proteins Infect Immun 61, 1346–1351.Google Scholar
  50. 46.
    Stevens, M K., Porcella, S, Klesney-Tan, J, Lumbley, S., Thomas, S E, Norgard, M. V, Radolf, J D., and Hansen, E. J. (1996) A hemoglobin-binding outer membrane protein is involved in virulence expression by Haemophilus ducreyi in an animal model. Infect Immun 64, 1724–1735.PubMedGoogle Scholar
  51. 47.
    Totten, P. A and Stamm, W E (I994) Clear broth and plate media for culture of Haemophilus ducreyi J Clin Microbiol 32, 2019–2023PubMedGoogle Scholar
  52. 48.
    Southern, E. M. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis J Mol Biol 98, 503–517PubMedCrossRefGoogle Scholar
  53. 49.
    Fernberg, A. P. and Vogelstein, B (1983) A technique for labeling DNA restriction endonuclease fragments to high specific activity Anal Biochem 132, 6–13CrossRefGoogle Scholar
  54. 50.
    Stacy-Phillips, S, Mecca, J J, and Weiss, J B. (1995) Multiplex PCR assay and simple preparation method for stool specimens detect enterotoxrgenic Escherichia coli DNA during course of infection. J Clin Microbiol 33, 1054–1059Google Scholar
  55. 51.
    Brenner, D. J., McWhorter, A. C, Leete Knutson, J. K., and Stelgerwalt, A. G (1982) Escherichia vulneris. a new species of Enterobacterraceae assocrated with human wounds. J Chn Microbiol 15, 1133–1140Google Scholar
  56. 52.
    Sambrook, J., Fritch, E. F, and Maniatis, T (1989) Molecular Clonzng A Laboratory Manual, 2nd ed, Cold Spring Harbor Laboratory, New YorkGoogle Scholar
  57. 53.
    Ausubel, F M, et al (ed) (1995) Current Protocols in Molecualr Biology, Wiley, New York.Google Scholar
  58. 54.
    Grimont, F, and Grimont, P A D (1991) DNA fingerprinting, in Nucleic Acid Techniques in Bacterial Systematics (Stackebrandt, E and Goodfellow, M, eds ), Wiley, Chlchester, England, pp 247–279Google Scholar
  59. 55.
    Picard-Pasquter, N, Quaqued, M, Picard, B, Goullet, P., and Krishnamoorthy, R. (1989) A simple sensitive method of analyzing bacterial ribosomal DNA polymorphism. Electrophoresis 10, 186–189.CrossRefGoogle Scholar
  60. 56.
    Brosms, J., Ullrich, A., Raker, M. A, Gray, A, Dull, T J, Gutell, R R., and Noller, H. F (1981) Constructron and fine mapping of recombinant plasmids containing the rrnB nbosomal RNA operon of Escherichia co1i Plasmid 6, 112–118CrossRefGoogle Scholar
  61. 57.
    Altwegg, M, Altwegg-Bissig, R, Demarta, A, Peduzzi, R, Reeves, M W, and Swaminathan, B (1988) Compartson of four typing methods for Aeromonas species J Diarrhoeal Dis Res 6, 88–94.PubMedGoogle Scholar

Copyright information

© Humana Press Inc. 1998

Authors and Affiliations

  • Stephen A. Morse
    • 1
  • David L. Trees
    • 1
  • Patricia A. Totten
    • 2
  1. 1.Division of A1DS, STD and TB Laboratory ResearchNational Center for Infectious Diseases, Centers for Disease Control and PreventionAtlanta
  2. 2.Division of Infectious DiseasesUniversity of Washington, Harborview Medical CenterSeattle

Personalised recommendations