Abstract
The rapid diagnosis of infectious diseases, particularly those because of their communicability present a public health problem, is the objective of the clinical microbiologist and the practicing physician. The need to attain this goal is exemplified by the resurgence of tuberculosis and the increasing prevalence of drug-resistant isolates of Mycobacterium tuberculosis. The recent introduction of molecular technologies for the laboratory diagnosis of infectious diseases into the clinical microbiology laboratory now makes the goal of truly rapid diagnosis of infectious diseases attainable.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Boddinghaus, B., Rogall, T., Flohr, T., Blocker, H., and Bottger, E. C. (1990) Detection and identification of mycobacteria by amplification of rRNA. J. Clin. Microbiol. 28, 1751–1759.
Stahl, D. A. and Urbance, J. W. (1990) The division between fast-and slow-growing species corresponds to natural relationships among the mycobacteria. J. Bact. 172, 116–124.
Longo, M. C., Berninger, M. S., and Hartley, J. L. (1990) Use of Uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions. Gene 93, 125–128.
Kent, P. T. and Kubica, G. O. (1985) US DHHS. Public Health Mycobacteriology. A guide for the Level III laboratory. Centers for Disease Control, Atlanta, GA.
D’Amato, R. F., Wallman, A. A., Hochstein, L. H., Colaninno, P. M., Scardamaglia, M., Ardila, E., Ghouri, M., Kim, K., Patel, R. C., and Miller, A. (1995) Rapid diagnosis of pulmonary tuberculosis by using roche amplicor Mycobacterium tuberculosis PCR test. J. Clin. Microbiol. 33, 2906–2908.
Abe, C., Hirano, K., Wada, M., Kazumi, Y., Takahashi, M., Fukasawa, Y., Yoshimura, T., Miyagi, C., and Goto, S. (1993) Detection of Mycobacterium tuberculosis in clinical specimens by polymerase chain reaction and Gen-Probe Amplified Tuberculosis Direct Test. J. Clin. Microbiol. 31, 3270–3274.
Beavis, K. G., Lichty, M. B., Jungkind, D. L., and Giger, O. (1995) Evaluation of Amplicor PCR for direct detection of Mycobacterium tuberculosis from sputum specimens. J. Clin. Microbiol. 33, 2582–2586.
Patel, R. C., Miller, A., Kim, K., Hochstein, L., D’Amato, R. F. (1995) Specificity of the PCR based Amplicor™ Mycobacterium tuberculosis test: Is there cross-reactivity with MOTT? Results from 108 culture-positive specimens for MOTT. Am. J. Resp. Crit. Care Med. 151, A476.
Vuorinen, P., Miettinen, A., Vuento, R., and Hällström, O. (1995) Direct detection of Mycobacterium tuberculosis complex in respiratory specimens by Gen-Probe amplified Mycobacterium Tuberculosis Direct Test and Roche Amplicor Mycobacterium Tuberculosis Test. J. Clin. Microbiol. 33, 1856–1859.
Wobeser, W. L., Krajden, M., Conly, J., Simpson, H., Yim, B., D’Costa, M., Fuksa, M., Hian-Cheong, C., Patterson, M., Phillips, A., Bannatyne, R., Haddad, A., Brunton, J., and Kraiden, S. (1996) Evaluation of Roche Amplicor PCR assay for Mycobacterium tuberculosis. J. Clin. Microbiol. 34, 134–139.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Humana Press Inc.
About this protocol
Cite this protocol
D’Amato, R.F., Miller, A. (1998). Rapid Diagnosis of Pulmonary Tuberculosis Using Roche AMPLICOR™Mycobacterium tuberculosis PCR Test. In: Meltzer, S.J. (eds) PCR in Bioanalysis. Methods In Molecular Medicine™, vol 92. Humana Press. https://doi.org/10.1385/0-89603-497-6:203
Download citation
DOI: https://doi.org/10.1385/0-89603-497-6:203
Publisher Name: Humana Press
Print ISBN: 978-0-89603-497-6
Online ISBN: 978-1-59259-575-4
eBook Packages: Springer Protocols