The SURF Technique

Selective Genetic Analysis of Microscopic Tissue Heterogeneity
  • Darryl Shibata
Part of the Methods In Molecular Medicine™ book series (MIMB, volume 92)

Abstract

Selective ultraviolet radiation fractionation (SURF) is a simple technique for the isolation of histologically defined microscopic tissue regions (1,2). Very small numbers (100–400) of cells can be rapidly isolated with relatively crude equipment. The isolated cells can be analyzed genetically by PCR, thereby allowing a direct comparison between microscopic phenotype with genotype. The ability to compare genotype between different tissue areas provides opportunities to analyze many disease processes, including the heterogeneity expected of multistep tumor progression.

Keywords

Formalin EDTA Electrophoresis Fractionation Paraffin 

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Copyright information

© Humana Press Inc. 1998

Authors and Affiliations

  • Darryl Shibata
    • 1
  1. 1.Department of Pathology, School of MedicineUniversity of Southern CaliforniaLos Angeles

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