Abstract
Antibodies have played a key role in receptor research. These immunoglobulms (Ig) frequently have greater affinities and selectivities for receptors than pharmacological probes. They are often the only probes available that can discern proteins with similar structural and pharmacological properties, such as distinct isoforms or splice-variants of a receptor family. Furthermore, antibodies may be utilized to determine a wide range of a receptor’ characterrstrcs: its apparent molecular mass by immunoblotting; its interaction with other proteins, covalent modifications, and turnover rates by immunoprecipitation; its localization in tissues, cells, and subcellular domains by immunohistochemistry, immunocytochemtstry, and immunoelectron microscopy; its primary structure by means of screening complementary DNA (cDNA) expression libraries; and its functional properties by virtue of some antibodies acting as receptor agonists/antagonists.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Kohler G. and Milstein C (1975) Contmuous cultures of fused cells secreting antibody of defined specificity. Nature (London) 256, 495–497.
Goding J. W. (1987) Monoclonal Antibodzes. Principles and Practice. 2nd ed. Academic, London
Winter G. Griffths A. D. Hawkins R. E., and Hoogenboom H R. (1994) Making antibodies by phage display technology. Ann. Rev Immunol 12, 433–455.
Walter G. (1986) Production and use of antibodies against synthetrc peptides. J. Immunol. Methods 88, 149–161
Jameson B. A. and Wolfe H. (1988) The antigenic index a novel algorithm for predicting antigenic determinants. CABIOS 4, 18l–l86
van Regenmortel M. H. V. Briand J. P. Muller S., and Plaué S. (1988) Synthetic Peptides as Antigens. Elsevier, New York.
Sambrook J. Fritsch E. F., and Mamatis T. (1989) Molecular Clontng A Laboratory Manual 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Freund J. (1956) The mode of action of immunologic adJuvants. Adv Tuberc Res. 7, 130–148.
Engvall E and Perlmann P (1972) Enzyme-linked immunosorbent assay, ELISA. III. Quantitation of specific antibodies by enzyme-labelled anti-immunoglobulin in antigen-coated tubes. J. Immunol. 109, 129–135.
Ey P. L. Prowse S. J., and Jenkin C. R. (1978) Isolation of pure IgG1, IgG2a, and IgG2bb immunoglobulins from mouse serum using protein A sepharose. Btochemtstry 15, 429–436.
Smith D. E and Fisher P. A. (1984) Identification, developmental regulation, and response to heat shock of two antigenically related forms of a maJor nuclear envelope protein in Drosophila embryos: application of an improved method for affinity purification of antibodies usmg polypeptides immobilised on nitrocellulose blots. J CellBtol. 99, 20–28.
Evans W. H. (1992) Isolation and characterization of membranes and cell organelles, in Preparative Centrifugation. A Practical Approach (Rickwood, D., ed.), IRL, Oxford, pp. 233–270.
Beisegel U. (1986) Review: protein blotting. Electrophorests 7, 1–18.
Riggs P. (1992) Current Protocols in Molecular Biology (Ausubel, F. M. et al., eds.), GreeneAssociates/Wiley Interscience, New York.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1997 Humana Press Inc.
About this protocol
Cite this protocol
Mackrill, J.J. (1997). The Generation of Receptor-Selective Antibodies. In: Challiss, R.A.J. (eds) Receptor Signal Transduction Protocols. Methods in Molecular Biology™, vol 83. Humana Press. https://doi.org/10.1385/0-89603-495-X:65
Download citation
DOI: https://doi.org/10.1385/0-89603-495-X:65
Publisher Name: Humana Press
Print ISBN: 978-0-89603-495-2
Online ISBN: 978-1-59259-567-9
eBook Packages: Springer Protocols