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The Xenopus Egg Extract Translation System

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 86))

Abstract

A full analysis of the post-translational modifications that a given protein undergoes during transit through the secretory pathway may, in some cases, only be performed by analysis of the natural protein, expressed in its normal tissue.

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References

  1. Matthews G. M. and Colman A (1991) A highly efficient, cell-free translation/translocation system prepared fromXenopus eggs. Nucleic Acids Res. 19, 6405–6412.

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  2. Murray A. W. (1991) Cell cycle extracts, in Methods in Cell Biology, vol. 36 (Kay B. K. and Peng H. B., eds.), Academic, San Diego, pp. 581–605.

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  3. Kreig P. A. and Melton D. A. (1984) Functional messenger RNAs are produced by SP6 in vitro transcnptlon of cloned cDNAs Nucleic Acids Res 12, 7057–7070.

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  4. Colman A. (1984) Translation of eukaryotic messenger RNA in Xenopus oocytes, in Transcription and Translation-A Practxal Approach (Hames B. D. and Higgins S. J., eds.), IRL, Oxford, pp. 271–302.

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© 1998 Humana Press Inc.

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Matthews, G.M., Colman, A. (1998). The Xenopus Egg Extract Translation System. In: Rapley, R., Manning, D.L. (eds) RNA Isolation and Characterization Protocols. Methods in Molecular Biology™, vol 86. Humana Press. https://doi.org/10.1385/0-89603-494-1:235

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  • DOI: https://doi.org/10.1385/0-89603-494-1:235

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-494-5

  • Online ISBN: 978-1-59259-570-9

  • eBook Packages: Springer Protocols

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