Abstract
Unlike gene expression in procaryotic cells, which is primarily under transcriptional control, gene expression in eucaryotic cells is subject to both transcriptional and post-transcriptional controls. Since transcription and translation in eukaryotic cells are separated topographically, the regulation of mRNA metabolism can occur at multiple sites, within nuclei and in the cytoplasm. Nevertheless, transcription remains a critical locus of control of eukaryotic gene expression. Transcriptional regulation affects cellular mRNA abundance by affecting rates of transcription. Another important control mechanism that can affect mRNA abundance is the rate of mRNA decay. Thus, half-life of mRNA represents a balance between the rates of transcription and intracellular degradation, e.g., an increase in the abundance of mRNA could result from decreased mRNA degradation, increased mRNA synthesis, or both.
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Srivastava, R.A.K., Schonfeld, G. (1998). Measurements of Rate of Transcription in Isolated Nuclie by Nuclear “Run-Off” Assay. In: Rapley, R., Manning, D.L. (eds) RNA Isolation and Characterization Protocols. Methods in Molecular Biology™, vol 86. Humana Press. https://doi.org/10.1385/0-89603-494-1:201
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DOI: https://doi.org/10.1385/0-89603-494-1:201
Publisher Name: Humana Press
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